1株奶牛源松鼠葡萄球菌的分离鉴定及耐药性分析

为确定奶牛垫料中是否含有病原菌并深入了解垫料中优势生长菌的类型、耐药性及致病性等情况,本试验进行了细菌分离培养、革兰染色镜检、生化试验鉴定、16S rDNA序列分析及同源性比对、药敏试验、小鼠致病性试验。结果显示,分离菌在普通营养琼脂平板上形成圆形、表面光滑的白色菌落,血琼脂平板上形成黏稠、较大的白色菌落。革兰染色镜检结果显示,分离菌为革兰阳性,球型,呈葡萄串状排列,或单个散落。生化试验结果显示,分离菌对木糖、葡萄糖、麦芽糖、果糖、乳糖、硝酸盐还原等呈阳性反应,而蜜二糖、木糖醇、山梨醇、尿素、V-P反应等呈阴性反应。16S rDNA序列分析结果显示,扩增的16S rDNA序列长度为1 298 bp,与松鼠葡萄球菌的核苷酸同源性达99.85%~100%;系统进化树结果显示,分离菌与松鼠葡萄球菌处于同一分支。动物试验结果表明,分离菌株对试验小鼠有较强的致病性,以0.2 mL/只（7.9×10⁸ CFU/mL）菌液的剂量接种小鼠,在48 h内死亡率为60%（3/5）。药敏特性分析结果显示,分离菌株对复方新诺明、氨苄西林等7种药物敏感,对克林霉素、头孢噻肟和头孢呋辛中度敏感,对青霉素、红霉素和林可霉素耐药。本研究为奶牛源松鼠葡萄球菌的分离鉴定及防控提供了参考依据。     

Establishment and Application of a Multiplex RT-PCR Assay for Detection of PEDV,TGEV and PRoV

In this study,a multiplex RT-PCR assay was established to differentially detect porcine epidemic diarrhea virus (PEDV),porcine transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PRoV) after optimization of the reaction conditions.Three pairs of primers PEDV-N,TGEV-M and PRoV-VP6 were designed for specifically amplifying PEDV N gene,TGEV M gene and PRoV VP6 gene,respectively.The assay could specifically amplify PEDV,TGEV and PRoV,but not classical swine fever virus (CSFV),porcine foot and mouth disease virus (FMDV),pseudorabies virus (PRV),porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2).The detection limits of PEDV,TGEV and PRoV standard recombinant plasmids were 1.41×10³,1.41×10² and 1.41×10³ copies/μL,respectively.The repeated reaction under the same conditions obtained uniform results.The assay was used to detect a total number of 190 clinical samples,of which 42 (22.11%) samples were positive for PEDV,58 (30.53%) samples for TGEV and 34 (17.89%) samples for PRoV,and there were mixed infection among these viruses.The results indicated that this multiplex RT-PCR assay had the advantages of sensitivity,specificity and repeatability and provided a useful tool for differential detection and epidemiological investigation of PEDV,TGEV and PRoV.     

日光温室分段智能屋脊通风效果研究

针对现有日光温室人工通风方式劳动强度大、室内气温分布不均匀等问题,在温室内安装2台或3台智能型屋脊通风设备对温室进行2段或3段智能通风,并在北京地区冬季日光温室与人工通风控制进行了对比。结果表明:在保温被揭开期间,当采用3段智能通风时,温室内空气温度最大值与最小值之差为(1.1±0.5)℃;而2段智能控制和人工控制分别为(2.3±1.1)℃和(3.8±1.3)℃。此外,应用该智能屋脊通风技术,还能将667m2均效益提高0.97万元。因此,智能型屋脊通风设备可显著改善温室内温度分布的均匀性,提高种植的经济收益。     

我国果树玻璃化试管苗特征、成因、机理及控制研究进展

该研究统计了我国已报道的出现玻璃化试管苗的果树种类多达50种,并基于近年果树工作者的相关研究,总结并系统论述了玻璃化试管苗的生理生化特性、形态解剖学特征、DNA的MSAP分析、成因、发生机理及控制措施,对系统研究果树试管苗玻璃化现象、增加转基因植株的遗传稳定性、保存和恢复珍贵材料无性系、提高次生代谢物产量和消除毒性及工厂化育苗都具有十分重要的意义。     

地熊蜂为重庆地区设施番茄授粉的效果研究

为评价地熊蜂在重庆地区设施番茄的授粉效果,在重庆市荣昌区龙舌嘴村开展授粉效果比较试验,分析地熊蜂授粉对设施番茄产量、果实品质及畸形果率的影响。试验结果显示,经地熊蜂授粉的设施番茄平均产量为2 206.44 kg/667 m2,与未授粉组相比显著提高了104.95%,授粉组的单果质量、果纵径、果最大横径、可溶性固形物含量、VC含量及总糖含量显著高于未授粉组。由此,认为地熊蜂适宜为重庆地区设施番茄授粉,且能够显著提高重庆地区设施番茄的产量与品质。     

外源ACC通过激活乙烯合成及信号转导诱导小豆抗锈性

大量研究表明, 乙烯可激发植物对死体营养型真菌的抗性, 但我们前期研究发现, 乙烯合成前体ACC可提高小豆对活体营养型真菌——锈菌的抗性, 为初步明确其机制, 本研究分析了ACC处理对小豆乙烯合成及信号转导的影响, 结果表明, ACC处理显著提高了乙烯合成基因VaACS1及信号通路关键基因VaEIN2?VaEIN3?VaERF5的表达水平?此外, ACC处理后再接种锈菌, 小豆锈病的发病程度显著降低?对接种锈菌后不同时间VaPR2和VaPR4的表达分析表明, 相比ACC处理后不接种对照, ACC处理后再接种锈菌的处理, 接种后1~5 d这两个基因表达量显著升高; 与水处理不接种锈菌相比, 水处理接种锈菌5~8 d后VaPR2和VaPR4的表达量虽显著上调, 但应答时间较ACC处理滞后, 且总体表达水平低, 表明ACC激活乙烯通路进而诱导防卫反应基因上调表达是其诱导小豆抗锈性的关键?     

水氮调控对苜蓿与无芒雀麦混播草地生长和辐射利用的影响

针对我国内陆干旱区人工草地生产管理粗放及气候资源利用不充分等问题,探究合理的牧草种植与水氮供应模式,以充分挖掘区域牧草的生产潜力。采用3 a生(2018年播种)紫花苜蓿(简称‘苜蓿’)和无芒雀麦,分析种植模式(苜蓿与无芒雀麦混播,D1;无芒雀麦单播,D2)、施氮量(低氮量N1:60 kg·hm^-2;高氮量N2:120 kg·hm^-2)和灌水量(以灌水下限占田间持水量θ_f的百分比计,分枝期均充分灌水(75%～85%θ_f),现蕾和初花期轻度亏水W1:65%～75%θ_f、中度亏水W2:55%～65%θ_f、重度亏水W3:45%～55%θ_f,灌水上限均为85%θ_f)对牧草叶面积指数(LAI)、干物质累积量、累积截获光合有效辐射量(CIPAR)、辐射利用效率(RUE)、产量(Y)、耗水量(ET_a)、水分利用效率(WUE)和氮肥偏生产力(PFP_N)的影响。结果表明：(1)牧草LAI和...     

Physiological but not morphological adjustments along latitudinal gradients in a human commensal species,the Eurasian tree sparrow

Human commensal species take advantage of anthropogenic conditions that are less likely to be challenged by the selective pressures of natural environments. Their morphological and physiological phenotypes can therefore dissociate from habitat characteristics. Understanding how these species adjust their morphological and physiological traits across latitudinal gradients is fundamental to uncovering the eco-physiological strategies underlying coping mechanisms. Here, we studied morphological traits in breeding Eurasian tree sparrows (ETSs; Passer montanus) among low-latitude (Yunnan and Hunan) and middle-latitude (Hebei) localities in China. We then compared body mass; lengths of bill, tarsometatarsus, wing, total body, and tail feather; and baseline and capture stress-induced levels of plasma corticosterone (CORT) and the metabolites including glucose (Glu), total triglyceride (TG), free fatty acid (FFA), total protein, and uric acid (UA). None of the measured morphological parameters varied with latitude except in the Hunan population, which demonstrated longer bills than those in other populations. Stress-induced CORT levels significantly exceeded baseline levels and decreased with increasing latitude, but total integrated CORT levels did not vary with latitude. Capture stress-induced significantly increased Glu levels and decreased TG levels, independent of site. However, the Hunan population had significantly higher baseline CORT, baseline and stress-induced FFA levels, but lower UA levels, which differed from other populations. Our results suggest that rather than morphological adjustments, physiological adjustments are mainly involved in coping mechanisms for middle-latitude adaptation in ETSs. It is worth investigating whether other avian species also exhibit such dissociation from external morphological designs while depending on physiological adjustments.     

GA3和PP333对萝卜生长及产量的影响

通过研究不同浓度的GA3和PP333及两激素不同浓度的互作对萝卜叶面积、叶重量、叶子叶绿素含量及根冠比的影响。结果表明，两者对叶子叶绿素含量没明显影响，但GA3在促进叶子的生长，叶面积的增大上以10~30ppm效果最好，而PP333虽然能抑制叶子的生长，但在肉质根生长中后期试用50ppm浓度的PP333，抑制了叶子的生长，反而促进了光合产物输送到肉质根，起到增产的作用。适当浓度的GA3和PP333及两者结合增产效果明显     

Frequency Refining Analysis and Its Applications in the VIs

Frequency refining is an important spectrum analyzing tool, and is one of the main function of signal processing and the virtual instrument. This paper introduces several typical frequency refining analysis methods including DFT and multiple modulation and so on. It also probes into their advantages and disadvantages, and proposes a new frequency refining method combining subband decomposing DFT and multiple modulation, which inherits the advantages of multiple modulation, needs not to design a specific filter, reduces the amount of calculation, and is fit for real-time refining analysis. At last, it exhibits several frequency refining algorithm and their effects with applications in virtual instruments.