Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms

By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.     

农业科研单位编制外用工的管理策略

编制外用工是农业科研单位普遍存在的用工形式。本文阐述了农业科研单位编制外用工的特点，分析了编制外用工存在问题及原因，提出从规范用工管理方式、强化考核管理、拓宽发展空间等方面提高编制外人员的工作积极性，提升编制外用工人力资源管理的效能，促进单位发展。     

云南芥菜型油菜资源品质及苗期耐旱性评价

为研究芥菜型油菜的耐旱性，对150份云南芥菜型油菜地方品种的品质及苗期不同干旱程度下植株地上 及地下部进行研究。结果表明，参试芥菜型油菜耐旱性总体表现较好，品质变异丰富，其中油酸、芥酸变异系数大 于1，依据品质性状参试品种大致聚为三类；中度及重度干旱均能显著影响油菜地上部及根系的生长，其中对根系 影响更大；各品质性状与耐旱性相关分析表明，中度干旱条件下，耐旱性与含油量及芥酸、花生烯酸2种极长链脂肪 酸呈显著正相关，与5种长链脂肪酸硬脂酸、棕榈酸、油酸、亚油酸、亚麻酸为显著负相关，而重度干旱条件下均不相 关，同一品种在不同程度干旱下其耐旱性有差别；含油量与极长链脂肪酸呈显著正相关，与长链脂肪酸及蛋白含量 呈显著负相关，极长链脂肪酸与长链脂肪酸间呈极显著负相关。共筛出8个综合耐旱性强的材料。     

甘蓝型油菜耐低温生长生理生化响应机制

为提高甘蓝型油菜耐寒育种过程中的筛选效率，研究甘蓝型油菜耐低温机理，以8个不同抗寒性的甘蓝型油菜为材料，对低温条件下各材料的生物量、叶绿素、脯氨酸和相对电导率进行测定，发现在室外平均气温2.75℃时，抗性材料的生物量、叶绿素和脯氨酸的累积量都显著高于敏感材料，相对电导率没有显著差异；当平均气温7.52℃回升至12.39℃，抗性材料和敏感材料生物量的累积量无显著差异；在恒定低温10℃/4℃处理下抗性材料和敏感材料在处理前3周生物量均持续累积，但从第4周开始敏感材料新叶出现白斑，生物量减少，抗性材料老叶出现白斑，生物量维持不变。结果表明，耐低温材料在低温条件下叶绿素含量受到的影响较小，脯氨酸的积累量持续上升，具有较强的快速适应能力，在低温下具有显著的持续生长优势。     

Whole-genome identiifcation and expression analysis of K+eflfux antiporter (KEA) and Na+/H+antiporter (NHX) families under abiotic stress in soybean

Sodium toxicity and potassium insufifcient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K+/Na+ratio is t...     

Co^2＋对非洲菊切花保鲜过程中酶活性的影响

基于测定过氧化物酶（POD）、超氧化物歧化酶（SOD）的活性以及丙二醛（MDA）含量,探讨Co^2＋对非洲菊（Gerbera jamesonii）切花的保鲜作用,实验结果：含有Co^2＋的切花保鲜液能有效提高POD、SOD活性,延缓MDA含量的上升。花径、花枝鲜重增长率及外部形态变化显现一致。结果表明Co^2＋可延缓非洲菊切花衰老,提高其观赏价值。     

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.