枇杷种质资源果实若干性状及相关性研究

对224份枇杷种质果实11个性状的分布情况及性状间的相关性进行了分析研究,结果表明：（1）枇杷种质资源果实的单果重、可溶性固形物含量、可食率、果实纵经、果实横径、果实侧径、果形指数、萼片长度、萼片基部宽度、萼筒宽度和深度等11个性状存在广泛的分布,变异系数在11.81%~48.92%之间,分布区间以单果重为最大,果形指数为最小;（2）果形指数不能完全反映果实形状;果形指数在0.91~1.10之间的种质果形最为丰富;（3）单果重与果实纵径、果实横径、果实侧径、萼片长度、萼片基部宽度、萼筒深度、可食率及果形指数等8个性状呈极显著正相关,与可溶性固形物含量呈极显著负相关;可食率与单果重、果实纵径、果实横径、果实侧径、萼片长度、萼片基部宽度、萼筒深度及果形指数等8个性状呈极显著正相关,与可溶性固形物含量呈显著负相关;果形指数与果实纵经、单果重、萼筒深度、可食率等4个性状呈极显著正相关,与可溶性固形物含量呈极显著负相关。     

甜橙新品种——桂橙一号的选育

桂橙一号是冰糖橙优良芽变株系,成熟期11月下旬,平均单果质量154.0g,大小较均匀,可食率75.66%,可溶性固形物含量14.6%,全糖12.47%,酸0.38%,风味浓郁,有蜜香,甜脆化渣,树形开张,丰产稳产,商品性好,适应性强,果实耐贮藏。     

中小果型无籽西瓜新品种博帅的选育

博帅系湖南博达隆科技发展有限公司以9048为母本、M-2为父本杂交育成的中小果型黄瓤无籽西瓜新品种。该品种全生育期90d左右,雌花开放至果实成熟约需27d。植株生长势中强,抗病抗逆性强。果实短椭圆形,果形指数1.2。果皮浅绿底覆绿色齿条带,厚度0.9cm,硬韧,耐贮运。瓤色鲜黄,质脆爽口,纤维少,无籽性能好;中心可溶性固形物12%～13%,边部9%～9.5%。单果质量4kg左右,667m2产量2700kg左右。适应性广,全国各地均可栽培。2009年3月通过湖南省农作物品种审定委员会审定,审定编号:湘审西瓜2009002。     

甜瓜枯萎病接种方法比较试验初报

分别采用灌根法、米饭粒接种法、蘸根法及胚根接种法对甜瓜枯萎病进行接种试验,拟为甜瓜抗病育种提供依据。结果表明:米饭粒接种法发病率最高,达到99%;胚根接种法最低,为10%;灌根法发病率为66.7%;蘸根法发病率为91%,且操作简单、精确,最适合进行甜瓜枯萎病抗病鉴定研究。     

基于可视化的Word办公报表的设计

作为信息化系统的重要组成部分,报表集成了企事业单位日常办公管理中的数据收集、数据处理和格式输出三个环节.针对现行Word报表系统中的模板建模和复杂报表的处理能力不足,通过Word XML(Extensible Markup Language)方案和基于单元格的处理,设计了一个具有良好可视化的Word办公报表系统.该系统有效地提高了报表模板建模的可视化和可操作性,降低了报表的计算复杂度.     

基于多载荷无人机遥感的大豆地上鲜生物量反演

以多载荷无人机获取数据和地面实测的数据为基础,将大豆生殖生长期分段建模,采用植被指数和光谱参数相结合再加上农学参数株高,通过最小二乘法建立多元线性回规模型的方法,来估算大豆开花期和结荚期的鲜生物量,采用高光谱植被指数法估算大豆鼓粒期和成熟期的鲜生物量。结果表明:在大豆开花期和结荚期内,采用混合法构建生物量反演模型利用交叉验证法,验证结果的R~2和RMSE分别为0.714和0.393;在大豆鼓粒期和成熟期内,采用高光谱植被指数法构建生物量反演模型,利用交叉验证法,验证结果的R~2和RMSE分别为0.697和0.386;大豆开花结荚期构建的模型和鼓粒成熟期构建的模型都有比较高的精度和可靠性,利用这两种模型完成了高光谱影像鲜生物量的遥感空间制图,能反映当地当时大豆的真实长势情况。     

Clinical significance of KLF15 expression in human lung adenocarcinoma

AIM: To explore the clinical significance of Krüpple-like factor 15 (KLF15) protein expression in the patients with lung adenocarcinoma for exploring the therapeutic and prognositic biomarkers of lung cancer. METHODS: Four cases of lung adenocarcinoma tissues and matched adjacent tissues were collected from our hospital, and the expression of KLF15 protein in these tissues was analyzed by Western blot. At the same time, 72 cases of archived paraffin-embedded samples and clinical data of the patients with lung adenocarcinoma were also collected. The KLF15 protein expression in the archived paraffin-embedded lung adenocarcinoma samples was detected by immunohistochemical staining. The correlations between KLF15 protein expression and clinical characteristics of the patients including prognosis were also analyzed. In addition, the KLF15 protein was up-regulated in A549 cells, and then the effects of KLF15 protein on the viability of the cells were measured by CCK-8 assay. RESULTS: The protein expression of KLF15 in the 4 cases of lung adenocarcinoma tissues was significantly lower than that in matched paracancerous tissues. Fifty-three cases of lung adenocarcinoma specimens showed low expression or no expression of KLF15 protein in total 72 cases (73.6%). The 5-year survival rate of the patients with high expression of KLF15 protein in their specimens was higher than that of the patients with the low expression of KLF15 protein (P<0.01), and the expression of KLF15 protein was significantly correlated with the pathological staging (P<0.01) and T stage (P<0.01) of the patients with lung adenocarcinoma. Furthermore, the low expression of KLF15 protein was an important poor prognostic indicator of the patients. Up-regulation of KLF15 protein in the A549 cells significantly inhibited the growth of the cells. CONCLUSION: KLF15 inhibits the growth of lung adenocarcinoma cells. It could be used as a therapeutic target and a prognostic biomarker for the patients with lung adenocarcinoma.     

Model construction of rat coronary artery smooth muscle cell endoplasmic reticulum stress induced by thapsigargin

AIM: To investigate the primary culture method for coronary artery smooth muscle cells (CASMCs), and to establish the endoplasmic reticulum stress (ERS) model in CASMCs of SD rats. METHODS: CASMCs were cultured by tissue explant method. The morphological characteristics were observed under optical microscope. The marker proteins of CASMCs, including α-SMA and SM-MHC, were identified by immunofluorescence technique. The protein expression levels of BiP and CHOP, the marker molecules of ERS, were determined by Western blot. RESULTS: The spindle-shaped CASMCs climbed out from the edge of coronary artery tissues after 6 d, and formed the typical "hill and valley" growth pattern of CASMCs at 9~10 d. The result of immunofluorescence technique showed that α-SMA and SM-MHC were positively expressed. The results of Western blot showed that the protein expression of BiP and CHOP in TG (1 and 2 μmol/L) treatment groups was increased compared with control group. Compared with control group, the protein expression of BiP and CHOP was significantly increased after 1 μmol/L TG treatment for 24 and 48 h. CONCLUSION: CASMCs can be successfully cultured by tissue explant method. ERS model of CASMCs was established by 1 μmol/L TG treatment for 24 h.     

遵义市辣椒产业现状及发展战略

遵义市是中国辣椒之都,位于全国最大的辣椒主产区西南和华中的中心区域,也是全国辣椒消费重辣区,其交通便捷,环境优美,辣椒产业文化丰富。当前遵义辣椒产业正处在基地优化、技术升级和产业提升的关键时期,亟需理清发展思路。通过对遵义辣椒产业现状的阐述与问题的分析,从而针对性地提出了发挥种植优势、突出产品特色,优化产区布局、推广生态化栽培,培育大型加工企业、促进加工产业升级与转型等战略,以促进遵义辣椒产业的发展。     

FSAE赛车发动机进气系统设计

文章主要对FSAE赛车发动机进气系统进行分析,从而在符合大赛规则的前提下设计出符合FSAE赛车高速性能要求的进气系统,即在赛车发动机进气系统中安装稳压腔.首先利用CATIA软件绘制两种进气系统的3D模型(含有稳压腔和不含稳压腔),再将其导入到ANSYS软件中进行模拟仿真分析,通过比较分析证明带稳压腔的进气系统在进气速度的均匀性与进气压力稳定性上均具有一定的优势.