椒参轮作缓解土壤酸化的效应

目的:比较太子参与辣椒轮作前后土壤pH值和酚酸类物质的变化,探讨椒参轮作对土壤酸化的影响.方法:将风干的土壤样品与去除CO2的超纯水按2:5的比例混合均匀,检测pH值;碱提酸沉后采用乙酸乙酯萃取法提取土壤样品中酚酸类物质,高效液相色谱法(HPLC)测定酚酸类物质指纹图谱.结果:太子参连作后,土壤pH值降低,酚酸类物质增加;与辣椒轮作后,土壤pH值相对提高,且酚酸类物质减少.结论:椒参轮作对太子参种植土壤酸化有一定的改善作用.     

采用混合生物地理学算法的番茄种植规划研究

为解决番茄酱产季原料供应不均衡问题,在构建番茄种植规划的数学模型基础上,提出采用生物地理学算法对番茄种植规划模型的求解。利用余弦迁移模型、干扰迁移算子和基于高斯分布的变异算子对BBO算法进行适当改进,并与差分进化算法的变异算子结合,以增强生物地理学算法的全局搜索性能。以新疆某番茄酱厂为例对番茄种植规划问题进行计算,结果表明:采用该算法获得的种植方案能实现番茄原料产量与番茄酱厂生产能力之间的平衡。仿真结果验证了番茄种植规划数学模型的合理性。在求解番茄种植规划问题上,与其他智能优化算法相比,该算法具有较好的收敛性。     

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.     

基于多元智能理论的多元评价体系及其在大学英语教学中的应用

教学评价是教学改革中很重要的一部分,也是保障和提高教学质量的有效手段之一.从多元智能理论的视角出发,提出了构建多元化大学英语评价体系的具体操作和实施步骤,总结了构建这一体系的现实意义.     

马蔺的花部构造及开花授粉生物学特性

对马蔺的花部特征、开花动态、授粉特性及传粉媒介等进行了研究,运用多种处理方式,对马蔺的传粉机制进行测定。结果表明:自然条件下,马蔺5～6月开花,群体花期约40d,盛花期约25d,单花花期约58h。马蔺的繁育系统属于异交,部分自交亲和,自然传粉需要借助传粉媒介,虫媒传粉是其传粉方式之一。     

利用Wood模型拟合中国荷斯坦牛乳脂率变化的效果分析

为了解中国荷斯坦牛乳脂率变化规律,本文利用Wood模型对我国南方5个大中型奶牛场2008~2010年1~3胎中国荷斯坦牛33 194条测定日乳脂率进行曲线拟合。结果表明:中国荷斯坦牛乳脂率变化曲线为典型的倒抛物线形,第一胎拟合度最高(0.9820),第三胎最低(0.9789)。二胎牛乳脂率最先达到最低点(第16周),而三胎乳脂率最后达到(第19周)。就最低乳脂率而言,二胎牛最大(3.54%),而三胎牛最小(3.45%)。综合各方面情况得出,Wood模型适用于中国荷斯坦牛乳脂率变化的曲线拟合。     

Overload of ferric ammonium citrate triggers MAPK pathways by producing high level of reactive oxygen species and induces apoptosis of human hFOB1.19 osteoblast cells

AIM：To investigate the role of reative oxygen species (ROS) generated by iron overload in activating the mitogen-activated protein kinase (MAPK) pathways and apoptosis. METHODS：Cultured human osteoblast cell line hFOB1.19 was treated with ferric ammonium citrate (FAC) at concentrations of 0~500 μmoL/L. The proliferation of hFOB1.19 cells was analyzed by MTT assay. Apoptosis was detected by flow cytometry with Annexin V/PI staining. The expression levels of p-ERK, p-JNK and p-p38 were determined by Western blotting 24 h after treatment with FAC. RESULTS：After treated with FAC, the cell proliferation was inhibited. The early apoptosis and total cell death were significantly increased. The levels of ROS were increased to (35.73±2.52)%, (62.89±4.24)% and (76.06±3.55)% with the increasing doses of FAC treatmen,respectively. The expression levels of p-ERK, p-JNK and p-p38 were also remarkably elevated in FAC groups. CONCLUSION：Iron overload increases intracellular ROS level, thus triggering the MAPK pathways and inducing apoptosis of human hFOB1.19 osteoblast cells.