全文获取类型
收费全文 | 8967篇 |
免费 | 614篇 |
国内免费 | 704篇 |
专业分类
林业 | 862篇 |
农学 | 400篇 |
基础科学 | 367篇 |
840篇 | |
综合类 | 4529篇 |
农作物 | 674篇 |
水产渔业 | 554篇 |
畜牧兽医 | 979篇 |
园艺 | 752篇 |
植物保护 | 328篇 |
出版年
2024年 | 56篇 |
2023年 | 183篇 |
2022年 | 393篇 |
2021年 | 432篇 |
2020年 | 359篇 |
2019年 | 374篇 |
2018年 | 255篇 |
2017年 | 424篇 |
2016年 | 239篇 |
2015年 | 429篇 |
2014年 | 481篇 |
2013年 | 547篇 |
2012年 | 768篇 |
2011年 | 729篇 |
2010年 | 741篇 |
2009年 | 628篇 |
2008年 | 689篇 |
2007年 | 636篇 |
2006年 | 499篇 |
2005年 | 443篇 |
2004年 | 233篇 |
2003年 | 152篇 |
2002年 | 183篇 |
2001年 | 174篇 |
2000年 | 143篇 |
1999年 | 42篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1981年 | 2篇 |
1966年 | 1篇 |
1962年 | 2篇 |
1956年 | 4篇 |
1955年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 882 毫秒
181.
CAI Chen-chen YE Li-xia ZHU Jiang-hu BAI Jun-jie ZENG Shan-shan CHEN Shang-qin LIN Zhen-lang 《园艺学报》2019,35(2):311-319
AIM:To investigate whether ellagic acid (EA) attenuates hypoxic-ischemic encephalopathy (HIE) by down-regulating autophagy. METHODS:In vivo, Sprague-Dawley rats (n=17) were randomly divided into 3 groups:5 rats for sham group, 6 rats for HIE group and 6 rats for HIE+EA pretreatment group. The rats in HIE+EA pretreatment group were treated with EA (10 mg/kg, 10 mL/kg, suspended in corn oil, ig). After 24 h of operation, the rats from each group were sacrificed and their brains were collected. TTC staining and HE staining were used to define the infarct areas and brain structure. The autophagy-related proteins beclin-1, P62, LC3-Ⅱ/-I and Atg5 in the cortex in each group were compared by Western blot. In vitro, PC12 cells were divided into 3 groups:control group, CoCl2 group and CoCl2+EA pretreatment group. CoCl2 at 800 μmol/L was added to the PC12 cells to induce an anoxic environment. The PC12 cells were pretreated with EA at 8 μmol/L and the cell viability was measured by CCK-8 assay. The production of reactive oxidative species (ROS) in the cells was detected by flow cytometry with DCFH-DA staining. MDC staining and TMRE staining were applied to reflect the extent of autophagy and the state of apoptosis, respectively. The autophagy-related proteins in PC12 cells were also investigated. RESULTS:In HIE group, 7-day-old rats were given the operations and the their large infarct areas in the hemisphere were observed by TTC staining. HE staining displayed the injured hemispheres which contained few neurons, and exhibited edema status and serious structural damage. EA pretreatment decreased the infarct area and alleviated the damage to hemisphere with more visible neurons, compared with HIE group. Compared with sham group, the levels of autophagy-related proteins Atg5, beclin-1 and LC3-Ⅱ/-I in the cortex were increased (P<0.01), and P62 protein expression was decreased (P<0.01) in HIE group. Compared with HIE group, the protein expression of Atg5, beclin-1 and LC3-Ⅱ/-I was decreased (P<0.01) and P62 protein expression was increased in HIE+EA pretreatment group (P<0.01). In vitro, compared with CoCl2 group, the PC12 cells in CoCl2+EA pretreatment group showed a lower ROS level. Moreover, the cells in CoCl2+EA pretreatment group exhibited higher mitochondrial membrane potential than that in CoCl2 group. MDC staining in CoCl2 group showed high value of fluorescence and increased number of autophagosomes. EA pretreatment reduced the number of autophagosomes and the extent of autophagy to protect PC12 cells. Furthermore, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I in CoCl2 group were higher (P<0.01), and the protein expression of P62 was lower (P<0.01) than those in control group. In CoCl2+EA pretreatment group, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I were decreased (P<0.01) and the protein expression of P62 was increased as compared with CoCl2 group (P<0.01). CONCLUSION:EA pretreatment attenuates autophagy to protect the neurons against HIE injury. 相似文献
182.
LI Ye-sheng LING Zhen-wei CHEN Qing-zi WU You-sen YE Qi-ting WEN Qi-rui DAI Yong-liang LI Jian-hua ZHOU Li-fen 《园艺学报》2019,35(9):1642-1647
AIM: To investigate the role of Toll-like receptor 4 (TLR4) and transient receptor potential channel 6 (TRPC6) signaling pathway in lipopolysaccharide (LPS)-induced nuclear factor-κB (NF-κB) P65 expression and nuclear translocation in airway epithelial cells (16HBE) for supplementing the mechanism for airway inflammation. METHODS: After stimulating the 16HBE cells with LPS at 1 mg/L for 0, 0.5, 2, 6, 12 and 24 h, the expression of NF-κB P65 at mRNA and protein levels in the 16HBE cells were determined by RT-PCR and Western blot respectively, and the nuclear translocation of NF-κB P65 was detected by immunocytochemical staining method. The effects of TLR4 inhibitor CLI-095 at 5 μmol/L and TRPC6 agonist Hyp9 at 10 μmol/L on LPS (1 mg/L)-induced NF-κB P65 expression and nuclear translocation in the 16HBE cells were determined by RT-PCR, Western blot and immunocytochemical staining. RESULTS: LPS increased the mRNA and protein expression of NF-κB P65 and nuclear translocation in the 16HBE cells(P<0.05). TLR4 inhibitor CLI-095 reduced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS, while Hyp9 enhanced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS in the 16HBE cells(P<0.05). CONCLUSION: LPS induces the expression and nuclear translocation of NF-κB P65 in the 16HBE cells via TLR4-TRPC6 signaling pathway. 相似文献
183.
开沟覆膜滴灌条件下土壤水、温变化规律研究 总被引:1,自引:0,他引:1
新疆是中国葡萄重要的生产基地,但每年因盐碱与缺水导致减产现象严重。开沟覆膜滴灌技术结合膜下滴灌与开沟技术优点,理论上可有效改善作物生长的水土环境。为研究桁架葡萄下开沟覆膜滴灌技术对土壤水分与温度变化的影响,在石河子147团6连试验地用EM50仪器开展土壤水分、温度监测试验。研究结果表明:①在开沟模式为20 cm×100 cm,灌水定额为300 m3/hm~2时,无论是膜中还是膜边,灌水前后土壤含水量维持在0.22~0.38,满足作物根系吸水。加大灌水定额,在覆膜影响下,滴灌带表面积水区面积增大,使得在滴头下方形成饱和区增大;随着开沟深度的增大,覆膜中土壤含水量变化不明显,覆膜边呈下降趋势。②温度监测表明,无论是温度上升期(12时),还是夜间下降期(24时),土体温度变化幅度均在15~31℃,给葡萄生长提供良好的温度环境,利于葡萄产量与品质的提高。 相似文献
184.
185.
基于ResNet-50深度卷积网络的果树病害智能诊断模型研究 总被引:2,自引:1,他引:1
[目的/意义]果树病害危及农业生产安全,运用人工智能技术帮助果农及时准确地识别果树病害对保障农业安全生产具有重要意义.[方法/过程]采用10 000张果树叶片病斑图像数据集,通过旋转、污化、增噪、切割等图像增强手段,提高样本图像的多样性;使用ResNet-50深度卷积网络模型,进行机器学习,获得果树病害识别模型,并基于... 相似文献
186.
首先以三氯化磷、二氯乙酸为原料,N,N-二甲基甲酰胺作催化剂,控制温度85℃,合成了重要反应原料二氯乙酰氯,产率60.8/。然后用氢氧化钠作缚酸剂、三氯甲烷为溶剂,利用二氯乙酰氯、乙醇胺和丁酮为原料,反应温度-4~4℃,搅拌1h,一锅法合成了除草剂安全剂3-二氯乙酰基-2-甲基-2-乙基-1,3-噁唑烷,产率78.6/。最后,对合成的化合物结构应用紫外光谱、红外光谱、核磁共振谱和气相色谱—质谱联机等分析方法进行表征,并对各种光谱谱图进行详细分析。根据光谱数据分析结果确定了除草剂安全剂3-二氯乙酰基-2-甲基-2-乙基-1,3-噁唑烷的结构式。 相似文献
187.
188.
189.
190.