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51.
AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE. 相似文献
52.
‘砀山酥梨’褐皮芽变木质素含量及相关酶活性与CCoAOMT 表达量分析 总被引:1,自引:0,他引:1
为了探讨‘砀山酥梨’芽变品系‘锈酥’果皮褐色形成机理,采用分光光度法测定盛花后25、50、75、100、125、150和175 d果皮中木质素含量和相关酶活性变化;从构建的‘锈酥’正向SSH-cDNA文库中筛选出与木质素生物合成密切相关的CCoAOMT-EST,通过实时荧光定量PCR测定了‘砀山酥梨’和‘锈酥’果皮中CCoAOMT的相对表达量。结果表明:‘锈酥’果皮发育前期木质素增量较大,且木质素增量累计比‘砀山酥梨’高12.2%;‘砀山酥梨’和‘锈酥’果皮中PAL、4CL、CAD酶活性均在花后75 d达到最大值,而POD酶活性则在花后125 d出现高峰;二者果皮中4种酶活性变化趋势基本一致,但‘锈酥’果皮中均相对较高;‘锈酥’果皮中的PAL和4CL酶活性与木质素增量变化均呈显著正相关,而‘砀山酥梨’则未呈现出此规律;在果实生长发育各个时期,‘锈酥’果皮中CCoAOMT相对表达量均高于‘砀山酥梨’。因此推测,‘锈酥’果皮褐色形成与果皮中木质素积累及相关酶活性提高有关,果皮中CCoAOMT的增量表达是‘锈酥’果实褐皮形成的重要原因之一。 相似文献
53.
近40年来托木尔峰南部地区气候及水文环境变化特征 总被引:3,自引:0,他引:3
西天山托木尔峰南部地区1987年以来出现了气候由暖干向暖湿转变趋势,在温度大幅上升的基础上,降水量和冰川融水量持续增加,相应的水文与生态环境也发生了显著的改善。本文在综合分析区域内水文、气象观测资料的基础上,对托木尔峰南部地区近40年来气温、降水以及径流量变化的基本趋势、不同海拔高度气温、降水变化差异,以及同一流域山区和平原区径流量变化的差异进行了深入分析。研究表明,受水汽输送途径和研究区地形地貌的影响,区域降水主要集中在托木尔峰南部山区;气候转型的原因是和北大西洋向东输送的水汽增加、西风气流加强有关;海拔2000m左右区域1987年以来气候向暖湿转化最为明显;近40年来由于气温升高,导致冰川消融补给河流量增加15%,但由于人类活动和粗放农业的发展,区域耗水量不断增加,导致塔里木河径流量不断减少,中下游生态环境急剧恶化。 相似文献
54.
C.?-A.?DedryverEmail author G.?Riault S.?Tanguy J.?F.?Le.?Gallic M.?Trottet E.?Jacquot 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(4):341-354
Vector efficiency of 44 clonal lines (clones) of Sitobion avenae belonging to 31 different genotypes (distinct patterns for five microsatellite loci) originating from Western France was evaluated by transmitting the isolate PAV4 of BYDV-PAV to barley seedlings. Variation in transmission rates from 3.7% to 92.5% was observed, with significant effects of the aphid clone, of the plant species on which clones were collected, and of the reproductive mode of the clones. When genotypes are considered instead of clones, a continuum in transmission rates was observed. A subset of S. avenae clones was tested for transmission of one (10 clones) and 13 (4 clones) other BYDV-PAV isolates, and a clear clone effect modulated by an isolate effect was observed. Crosses were made between clones with different vectoring phenotypes and their F1 progeny were tested for PAV4 transmission. The narrow sense heritability of the PAV transmission character was rather high in the F1 families (h2=0.5) and the segregation analyses suggested an oligo/polygenic determinism of this character. The possibility of generating new transmission variants by sexual reproduction and its consequences on transmission mechanism studies and on BYD epidemics are discussed. 相似文献
55.
利用1980年地形图、1988/1990年Landsat TM、2000年Landsat ETM+、2007年ALOS AVNIR-2遥感资料和近41年(1967-2007年)的气温、降水量资料对喜马拉雅东段洛扎地区的冰川、冰湖变化特征及变化原因进行了研究。结果表明:1)1980-2007年,本区冰川面积从491.64... 相似文献
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58.
Direct-fed microbials(DFMs) are feed additives containing live naturally existing microbes that can benefit animals’ health and production performance. Due to the banned or strictly limited prophylactic and growth promoting usage of antibiotics, DFMs have been considered as one of antimicrobial alternatives in livestock industry.Microorganisms used as DFMs for ruminants usually consist of bacteria including lactic acid producing bacteria,lactic acid utilizing bacteria and other bacterial groups,... 相似文献
59.
YE Jin-hao CHEN Jing JI Yang GU Jie-lei LIU Jian-wei LIU Shi-ming ZHONG Yun 《园艺学报》2000,36(8):1351-1358
AIM To investigate the effect of exosomes derived from hypoxia-preconditioned human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs). METHODS hUCMSCs and HUVECs were isolated, cultured and identified. Exosomes derived from hUCMSCs were extracted by ultracentrifugation. The morphological change of exosomes was observed under transmission electron microscope. The particle size and concentration of exosomes were detected by nanoparticle tracking analysis, and the surface specific marker proteins of exosomes were determined by Western blot. hUCMSCs were divided into normoxia group and hypoxia group. The viability of hUCMSCs was measured by CCK-8 assay. HUVECs were divided into control group, normoxic exosome group and hypoxic exosome group. The proliferation of HUVECs was detected by EdU assay. The migration ability was detected by cell scratch assay and Transwell experiment. Tube formation ability was evaluated by tube formation experiment. RESULTS Compared with normoxia group, hypoxia pretreatment enhanced the viability and exosome release of hUCMSCs. Compared with normoxic exosome group, hypoxic exosomes enhanced the proliferation, migration and tube formation of HUVECs. CONCLUSION Exosomes derived from hUCMSCs under hypoxia enhances the proliferation, migration and tube formation of HUVECs. 相似文献