Clinical significance of elevated serum interleukin 15 in patients with active lupus nephritis

AIM: To detect interleukin 15 (IL-15) levels in peripheral blood from patients with active lupus nephritis and investigate its clinical significance. METHODS: IL-15 level was determined by enzyme linked immunosorbent assay (ELISA). The peripheral blood mononuclear cells (PBMCs) were isolated with grads density abaxiality. The inhibitory effects of dexamethasone on production of IL-15, IgG and anti-dsDNA antibody in cultured PBMCs from LN patients were also investigated. RESULTS: (1) Serum IL-15 level in LN patients was significantly higher than that in normal controls (P<0.01). Serum IL-15 level in active LN patients was significantly higher than that in remised patients (P<0.05). (2) Serum IL-15 level was positively correlated with SLEDAI, anti-dsDNA antibody and 24 h urine protein excretion in active LN patients. (3) Serum IL-15 level was significantly reduced in LN patients treated with combination of cyclophosphamide (CTX) and steroid for 12 weeks. (4) Secretion of IL-15, IgG and dsDNA antibody in cultured PBMCs from active LN patients was significantly higher than that in normal control group, and IL-15 level in supernatant of cultured PBMCs from active LN patients was positively correlated with IgG and dsDNA antibody. Dexamethasone inhibited the secretion of IL-15, IgG and dsDNA antibody in cultured PBMCs from LN patients. CONCLUSION: Serum IL-15 level in patients with active lupus nephritis is significantly elevated, suggesting that IL-15 may be involved in the pathophysiological process of LN. IL-15 may be used as an index to assess the activity of LN.     

早熟丰产苦瓜长丰3号的选育

长丰3号苦瓜是汕头市白沙蔬菜原种研究所用C99-01A作母本、C01-03B作父本配制而成的杂交一代组合。果实长圆锥形,瓜长25～30cm,横径6～8cm,皮绿色,单瓜质量300～600g。中抗白粉病、枯萎病,耐热、耐寒、耐涝性强,适应性广。早熟,播种至初收春季为75天,夏秋直播后52天始收,每667m2产量3000～4000kg。     

畜禽有机复合肥对瓜类作物产量与品质的影响

研究了畜禽有机复合肥对瓜类作物产量与品质的影响。试验结果表明,基施嘉宝牌畜禽有机复合肥3.0～7.5t/hm2,瓜类作物收获后土壤速效K含量比CK增加155.59%～193.48%,同时显著提高了黄瓜的株高、茎粗、叶片数、功能叶面积和坐果率;黄瓜、西瓜和甜瓜产量随着有机复合肥施用量增加而增产,增幅为9.19%～27.16%,且黄瓜增产最为显著,黄瓜、西瓜和甜瓜的单位面积最佳经济施用量分别为7.5t/hm2、3.0t/hm2和4.5t/hm2;有机复合肥能提高黄瓜VC含量和西甜瓜中心糖度,显著改善其商品性和产品品质。     

不同处理对樱桃番茄贮藏特性的影响

用蔗糖基聚合物和施保克(咪鲜胺)对樱桃番茄进行采后处理.结果显示,施保克和蔗糖基聚合物处理能降低樱桃番茄的呼吸强度,抑制VC含量下降和过氧化物酶升高.另外,蔗糖基聚合物处理的果实SS,SPS的活性明显高于对照.高活性的SS和SPS有利于果实内糖分的积累.     

多菌灵对茶树菇菌丝生长的影响及其在子实体中的残留

研究了多菌灵对茶树菇和食用菌生产中3大主要污染霉菌菌丝生长的影响.在PDA培养基上多菌灵对茶树菇菌丝和孢菌、木霉及曲霉菌丝的EC50分别为22.3μg·mL-1、0.06μg·mL-1、0.29μg·mL-1和0.64μg·mL-1,MIC分别为60μg·mL-1、0.9μg·mL-1、1.6μg·mL-1和2.0μg·mL-1;在出菇袋料培养基中,按1500、11000和12000添加多菌灵对茶树菇菌丝生长影响不明显.同时采用液相色谱法分析了多菌灵在茶树菇子实体中的残留,3种处理的残留量分别为0.09ppm、0.04ppm和0.01ppm.     

木菠萝ISSR反应体系的建立和优化

以木菠萝为材料,研究了模板DNA、Taq DNA聚合酶、引物、Mg2+ 及dNTPs浓度对ISSR-PCR扩增结果的影响,得出木菠萝ISSR的较佳反应体系为:在20μI反应体系中DNA模板1.6 ng·μI-1,Taq DNA聚合酶1.25 U·(20μl)-1,引物0.24 μmol·L-1,dNTPs 0.2 mmol-1 ,Mg2+ 2 mmol· L-1,10×PCR缓冲液2.0μl.     

无核椪柑新品种——丽椪2号的选育

丽椪2号无核椪柑是有核或部分果实无核椪柑的芽变单株?闷分治藓寺蚀?98%,并且果形端正、扁平、平均单果质量140g左右,可溶性固形物含量11%,总糖9.0%,含酸量0.9%,可食率68%,风味浓,丰产性和商品性好、抗逆性强,果实耐贮藏。适宜种植范围为浙江及全国类似地区的有核椪柑适栽区。     

基于比较基因组学的簸箕柳DNA模体预测

以已完成全基因组测序及组装的模式生物拟南芥和具有很高研究价值的园艺植物柳树(Salix)的全基因组测序为数据源,采用基于位置的基因序列匹配方法,研究了新测序物种簸箕柳(Salix suchowensis)的DNA模体序列,以期为进一步了解柳树乃至林木的基因调控网络提供参考依据。结果表明:此次预测的结果与手工验证的部分簸箕柳的DNA模体一致,该研究发布的基于簸箕柳全基因组的DNA模体预测结果对于杨柳科植物的研究具有重要的意义。     

我国籽瓜的开发现状与研究进展

籽瓜是新疆重要的经济作物之一,在农业产业结构调整中占有重要地位。提高籽瓜的综合利用率,降低生产成本,提高附加值和经济效益,利用果肉和皮等废弃物开发籽瓜系列产品具有十分广阔的市场前景。该研究主要阐述了籽瓜产品的开发研究现状以及发展前景,以期为进一步深入研究籽瓜系列产品和加速其商品化进程奠定理论基础。