MB43活疫苗免疫效果及对鸡法氏囊组织操作的研究

本试验对鸡传染性法氏囊病（IBD）MB43活疫苗免疫后鸡的囊体比、新城疫血凝抑制（ND-HI）抗体效价及IBD AGP效价进行检测。结果表明，含有IBD高母抗试验鸡在17日龄免疫MB43活疫苗后，囊组织有不同程度的损伤；对正常免疫的ND-HI抗体效价无影响；试验鸡16天后IBD AGP阳性率为100%；21天攻毒保护率100%。     

福建山地杉木大径材定向培育技术

在闽北、闽中山地，通过对杉木大径材培育过程中的遗传控制、密度控制、立地控制与林分施肥等主要措施的试验研究．确定了杉木大径材定向培育的优化方案，即选用杉木优良家系，选择18指数以上的立地，初植密度1800～2505株／hm^2，间伐后保留密度1100～1200株／hm^2，可不施肥。为杉木大径材的目标经营提供科学依据。     

Effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS Thr495 phosphorylation in pulmonary artery endothelial cells

AIM: To examine the effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS activity in pulmonary artery endothelial cells (PAECs).METHODS: 1.Rat pulmonary artery (PA) rings of intrapulmonary arteries were suspended under isometric tension in oxygenated Krebs buffer in order to observe the differences on constriction induced by 15-HETE using nitro-L-arginine methyl ester (L-NAME, 10-4 mol/L, an inhibitor of eNOS) and removal of endothelium.2.The bovine PAECs was cultured for NO assay by Greiss reaction.3.Immunoprecipitation (IP) and Western blotting (WB) were used to analyze the cell extracts from bovine PAECs treated with 2×10-6 mol/L 15-HETE for 30 and 60 min, using phosphatase-eNOS (Thr495) antibody for IP and eNOS antibody for WB.RESULTS: 1.Inhibition of eNOS with L-NAME and removal of endothelium significantly enhanced 15-HETE induced contractions in rat PA rings in a concentration-dependent manner (P＜0.05, P＜0.01).2.15-HETE increased phospho-eNOS (Thr495) levels (P＜0.01) in cultured bovine PAECs.3.10-6 mol/L 15-HETE (P＜0.05) did significantly reduce nitrite production, whereas 10-5 mol/L cinnamy l 3, 4-dihydroxy-［alpha］-cyanocinnamate (CDC, P＜0.05), 10-4 mol/L nordihydroguiairetic acid (NDGA, P＜0.01) significantly increased nitrite production, as measured by a spectrophotometric assay.CONCLUSION: 15-HETE inhibits eNOS activity and decreases production of NO (NO-2/NO-3).The pathway of eNOS/NO is involved in 15-HETE-induced contraction in the rat PA.     

胶体染料试纸条法快速诊断家畜血吸虫病的研究

为了适应现场家畜血吸虫病的筛查需要,设计了简便快速的胶体染料试纸条诊断技术用于检测家畜血清中的抗血吸虫抗体,以该方法检测血吸虫病牛、猪血清83份,其结果为阳性检出率100%,健康牛、猪血清73份的检测结果为阴性符合率分别为95.3%和94.0%,与感染肝片形吸虫的牛、羊血清16份的交叉反应率为18.8%.表明本方法敏感性高,特异性较强.     

谈谈高校图书馆现采工作

从图书馆现场采购工作的优缺点入手，阐述了提高高校图书馆采购质量、不断完善现采工作必须从加强图书馆采购队伍建设、选择现采地点及现采工作中应注意的事项等方面着手。     

Intranet信息安全工作中客户端的防护

本文从内网客户端的软件、硬件和安全意识几个方面阐述了Intranet信息安全工作的重心和防护措施。     

黑茶镳子扦插育苗技术研究

对3个品种黑茶镳子‘坠玉’‘寒丰’‘绥研’的扦插技术研究表明,以当年生枝条为穗材,‘坠玉’用ABT 500 mg/L速蘸,生根率达43.3%,生根数达8~10条;‘寒丰’用ABT 200 mg/L或IBA 200 mg/L浸泡3 h,扦插生根率达100%,生根数大于30条;‘绥研’用ABT 500 mg/L速蘸3~5 s,扦插生根率达83.3%,生根数达8~10条。     

番石榴组织培养的初步研究

以番石榴无菌茎段为材料,研究了生长调节剂及MS强度对番石榴无菌茎段芽萌发和茎段生根的影响.结果表明,以MS为基本培养基时,最有利于番石榴无菌苗生长的NAA浓度为0.4 mg·L-1,叶片数(7.59片)、节数(3.95节)、苗高(5.10cm)和节间长度(1.41 cm)最大;6-BA浓度为5.0 mg·L-1时,茎段具有最高的芽诱导率(100％)和诱较高的芽诱导数(2.44个);1/2 MS+NAA 0.05 mg·L-1最有利于番石榴茎段的生根,生根率100％,根数2.30条,根长4.64 cm.在番石榴组织培养中,NAA能够促进番石榴茎段的生长,但会抑制芽的诱导.     

早熟高产优质抗病大豆新品种黑河19特征特性

黑河19大豆早熟、高产稳产、优质、抗病、秆强、喜肥水、不炸荚、适应性广，一般产量2488～2861kg／hm^2，增产幅度10．02％～13．90％，高产攻关或窄行密植可达3750kg／hm^2，2002～2004连续3年累计推广面积81万hm^2，增产大豆2．2亿kg。     

Sulphate Attack Resistance of the Concrete with Additives and Its Assessment

The influence of three kinds of additives: silica fume,slag and fly ash on the sulphate attack resistance of concrete were studied.The experiment results show that these three kinds of additives can improve sulphate attack resistance of the concrete and the order of their efficiency from high to low is silica fume,slag and fly ash;the development of mortar expansion value is closely related to the degree of sulphate attack resistance of the concrete;integrated assessing two concrete properties: mortar expansion value and compressive strength ratio can more exactly assess the sulphate attack resistance of the concrete.