陆地棉一个丝氨酸/苏氨酸激酶蛋白基因的克隆与表达分析

通过RT-PCR获得一个编码棉花丝氨酸/苏氨酸激酶类似蛋白全长的cDNA片段,其编码的氨基酸序列与拟南芥ATPK3的相似度达到82%,这个基因暂被命名为GhPK1。基因全编码区包括1038个核苷酸,编码346个氨基酸的蛋白。GhPK1的编码产物在265~270个氨基酸处有一个跨膜区并可能结合在内质网膜上。GhPK1在种子和纤维中的表达水平较其它组织高。另外,GhPK1的表达量在受到盐胁迫后上升,说明GhPK1可能参与了盐胁迫反应。     

VISAR Data Reducing Method and Its Application to Impact Experiment

Identification and adjustment on the disfigurements of original data of VISAR are probed by developing software for VISAR data reducing based on measure theory. Then the software is applied to plate impact experiment of ceramics. The disfigurements of light intensity data are identified and adjusted through Lissajou~ figures. The results indicate that VISAR data are reduced quickly and effectively by the software, and the exact free surface velocity history is obtained.     

Research and Development of a Quality Control System in Construction

In the civil industry, quality is a key factor, so quality management and quality control play decisive roles in construction especially. At present, the main method of construction is quality controlling that engineers and technicians consult the standard and norm concerned, then check them one by one, and supervise the construction. It is not only inefficient but also easy to make mistake. Using computer technology in the quality control of construction will promote quality control level and improve management measures. Through the research of the quality control of construction,the system of quality control in construction is studied and developed, the structure of the software and its developed technology are expounded.     

Electro-circuit Design About the Data Acquisition and Wireless Transmission System Based on High-speed SCM

Considering the difficult of acquiring and transmission the signal from the moving equipment, a new fault diagnosis monitoring system is presented. The system acquires the state signal from the moving equipment by data acquisition module that based in W77E58 high-speed SCM, and transmits the data fast by wireless mode. The signal data can be use for fault diagnosis and precise analysis. The system has the ability for acquiring abundance of data with a high speed, the ability for high accuracy data conversion, and has a function of programme controlling gain and programme controlling low-pass filtering. The successful application of this system on the vibration monitor about the material-transporting bogie in an iron works of a domestic steel enterprise proves the stability and credibility about the design project, also testifies its practical value and versatility.     

Effect of H2O2 in the Bioremediation of Petroleum Hydrocarbon Pollutant

The high efficient strain was separated and filtrated from the soil contaminated by petroleum hydrocarbon. The authors, we focus on the effect of H2 O2 in the bioremediation of petroleum hydrocarbon pollutant. The results show that the optimal concentration of H2O2was 200 mg/L at one time. At the lag phase of the strain, H2O2 was added in per eight hour, and at logarithmic phase, H2O2 was added in per two hour. Biodegradation rate of petroleum hydrocarbon was increased from 38. 1% to 83. 1%. The bioremediation of petroleum hydrocarbon pollutant is remarkably by double effects of deep oxidation and oxygen supply of H2O2.     

Deformation of deep foundation pits due to excavation and dewatering considering the change of void ratio and permeability coefficient with current stress of soil

基于修正剑桥模型理论,推导了孔隙比e随土体当前应力变化的方程,同时比选了4组经典的描述渗透系数k随孔隙比变化的方程,选择了其中一组最佳的估算公式,编写ABAQUS用户子程序VOIDRI和USDFLD,以实现孔隙比和渗透系数随土体当前应力的变化。在此基础上,研究深基坑降水开挖所致的坑内外土体的变形、围护结构的变形及弯矩,得到以下结论：当考虑孔隙比随土体当前应力变化时,坑外地表沉降量、墙体的水平位移、地下连续墙的弯矩、坑底隆起量均大于孔隙比为定值时的情况;当考虑渗透系数随土体当前应力变化时,坑外地表沉降量、墙体的水平位移、地下连续墙的弯矩均小于渗透系数为定值时的情况,但同时考虑渗透系数和孔隙比变化情况时,其对坑底的隆起量的影响可以忽略不计。     

The Reformation of the Construction Employment System and Building a Harmonious Society

Construction industry is not only the pillar industry in national economy,but also the typical industry with intensive labors.As a major part of the construction industry,the Construction Employment System(CES) is an important factor of the market order,as well as the quality,the safety and the development of the industry.It is also an important part for building a harmonious society.It concerns the social harmony,political harmony,societal harmony,cultural harmony,urban-rural harmony,etc.However,at present,there are still many problems in CES that might influence the harmonious society building.This paper advises to speed up the reformation of CES,to establish and consummate the system of construction labor subcontract,to develop vigorously labor subcontract corporations,to improve the quality of the labors,to ensure the rights and interests of the labor.