乙草胺降解菌Y-4的分离鉴定及降解特性研究

从长期经乙草胺污染的污泥中分离到一株能以乙草胺为唯一碳源和能源生长的菌株Y-4,通过生理生化实验和16S rDNA同源性序列分析,鉴定为申氏杆菌属（Shinella sp.）。采用室内培养方法,研究了Y-4对乙草胺的降解特性。结果表明,Y-4能有效地降解浓度为5～200 mg.L-1的乙草胺,在48 h内对50 mg.L-1乙草胺的降解率达到83.3%。菌株Y-4降解乙草胺的最适pH值为8.0,最适温度为30℃,其对丙草胺和丁草胺等农药也有良好的降解效果。     

树皮厚度和树皮系数变化规律的研究

研究树皮厚度和树皮系数的变化规律从而揭示树皮的变化规律,对提高林分蓄积生长量的精度有着重要的现实意义。通过实地测量树木的直径和树皮厚度,经过相关公式计算统计分析,结果表明,树皮厚度随直径的增大而呈现增加的趋势,树皮系数随直径的增大而呈现减小的趋势。桦树和山杨总树皮系数分别为1．093和1．078,其变动系数桦树O．94％,山杨为0．81％。     

浅谈榆林地区防沙治沙的策略

防沙治沙必须坚持可持续性、依法治沙、政府投资与群众的积极性相结合,创造出人与自然和谐发展的防沙治沙策略。     

Characterization and Genetic Analysis of Rumpled and Twisted Leaf Mutant(rtl1) in Rice

A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802,respectively.Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene.The closely linked simple sequence repeat(SSR) marker RM1155 was obtained from bulked segregant analysis.Subse...     

The finite element analysis on electromechanical behaviors in bone tissues

Osteoblast is looked as a homogeneous isotropic and viscoelastic body which can’t be compressed. Using the standard linear model of viscoelasticity, the matrix form of the relationship between stress and strain can be achieved, so the finite element balance equation can be got through Lagrange equation. An example for bone tissues on porous media board under loading is calculated, the history of displacement, velocity and stress are obtained. Then the osteoblast cultured invitro can be numerically analyzed by computer program, and the deformation, the velocity and the stress of osteoblast can be calculated.     

九种水生观赏植物无性繁殖试验

通过对宝鸡植物园的菖蒲、梭鱼草、芦苇等9种水生植物进行埋根段与单株繁殖试验,结果表明:菖蒲、梭鱼草、芦苇三种植物采用埋根段繁殖,方法简单,最高成活率可达100%;茭白、玉蝉花等六种植物采用单株带根、不带根繁殖,带根的成活率高于不带根的。     

干旱胁迫条件下信阳五月鲜桃光合特性和生理生化指标变化规律研究

以2年生信阳五月鲜桃幼树为试材,在盆栽条件下自然干旱形成水分梯度对信阳五月鲜桃幼树光合特性和生理生化特性进行研究,试验结果表明:信阳五月鲜桃光合速率日变化在清晨8∶00最大,随后逐渐下降,在中午14∶00降到最低,之后出现回升,有明显的"午休"现象;信阳五月鲜桃光合速率变化与蒸腾速率、气孔导度、叶片温度和胞间CO2浓度具有密切的关系;当土壤相对含水量大于55.7%时,信阳五月鲜桃光合速率降低的主要因素是气孔因素,当土壤相对含水量低于55.7%时,信阳五月鲜桃光合速率降低的主要因素是非气孔因素。当土壤相对含水量大于55.7%时,信阳五月鲜桃渗透调节物质不断积累,以SOD为主导的细胞保护酶系统活性增强,植株抗旱性机制明显提高。当土壤相对含水量低于55.7%时,水分亏缺加重,渗透调节能力减弱,质膜系统遭到破坏,质膜过氧化。     

Finite element simulation of duct grouting to standard prestressed concrete beam

For the duct grouting of prestressed beams,due to the narrow passageways grouting,the paste in the flow condition is very complex. In order to understand the grouting body in flow,the article establishes a three dimension finite element model of the grouting body,and uses the fluid mechanics software Fluent to analyze the grouting process of the standard prestressed beams through the finite element numerical method for the first time. The results show in the speed of cloud,the paste which is in a straight space and passes top space flows slow,may cause grouting defects. In the cloud of duct static pressure,the static pressure in the centre of the duct is greater than those on both ends. When paste is in hydration process,secrete water is forced to flow to the two ends,which causes grouting defects. The analysis results provide theoretical and actual testing data processing support for grouting nondestructive testing technology.     

Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu

High molecular weight glutenin subunit （HMW-GS） plays an important role in determining dough property and breadmaking quality, and the exploration of novel genes for HMW-GS will be crucial for quality improvement program. A gene coding the y type HMW-GS at Glu-A1 locus in Triticum urartu （AA, 2n=2×= 14） with an electrophoretic mobility similar to that of 1Dyl2, was cloned, sequenced, and heterologously expressed. This novel active lay gene FJ404595 was confirmed by structure analyses of nucleotide and deduced amino acid sequences combining with phylogenetic analysis. The open reading frame （ORF） of this gene was 1 830 bp, encoded a protein of 608 amino acid residues containing 46 hexapeptides and 14 nonapeptides, which was mostly similar to the lAy gene AM183223 at a high identity of 99.62% with the two substitutions of both leucine/proline and valine/glutamate, obviously different from the lAy gene EU984504 with 587 residues containing 44 hexapeptides and 13 nonapeptides in T. urartu. The amino acid （leucine） at 446 differed from that （proline） of all the eight compared active lAy subunits. The predicted secondary protein structure implied that this lAy subunit might also have positive impact on flour processing quality.     

Identification of UCHL1 as a potential biomarker for human gastric adenocarcinoma by subcellular proteomics

AIM: To search the biomarker for illustrating the pathogenesis of human gastric adenocarcinoma by differential proteome analysis of the cytosol of human gastric adenocarcinoma cell line SGC7901 and the corresponding immortalized normal gastric gland cell line GES. METHODS: The differential expression of cytosol protein between SGC7901 and GES cells was identified with subcellular protein separation and two-dimensional SDS-PAGE combined with MALDI-TOF-MS. The differential proteins were confirmed by Western blotting and semi-quantity RT-PCR. RESULTS: Ten repeatable differential proteins were identified. Compared with the expression level in normal gastric cell line GES, ubiquitin carboxyl-terminal hydrolase L1(UCHL1) was obviously down-regulated one both at protein and mRNA levels in gastric adenocarcinoma cell lines of SGC7901, AGS, BGC823 and MKN45. The expression of UCHL1 was also far lower in gastric cancer tissues than that in the corresponding normal tissues. CONCLUSION: Our finding suggests that UCHL1 has potential value as a biomarker for the diagnosis of gastric adenocarcinoma.