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181.
本试验旨在研究饲粮中添加银杏-杜仲叶发酵物(FGEL)对脂多糖(LPS)应激肉仔鸡的生长性能、肠道结构、消化吸收功能和免疫反应的影响,并与未发酵银杏-杜仲叶(GEL)进行比较。采用2×3双因子试验设计,即2种免疫应激处理(腹腔注射LPS或注射等量生理盐水)和3种饲粮处理(基础饲粮及在基础饲粮中添加0.40%GEL或0.40%FGEL的试验饲粮)。选取1日龄健康爱拔益加(AA)肉仔鸡360只,随机分为6个处理,每个处理均设6个重复,每个重复10只鸡。试验期为21 d。在试验第13、15、17、19和21天,3个应激处理的试鸡腹腔注射500μg/kg BW的LPS,其他3个处理注射等量的生理盐水。在试验第21天最后1次注射后2~4 h进行屠宰取样。结果表明:1)与对照饲粮相比,饲粮添加FGEL的肉仔鸡料重比(F/G)有显著改善(P=0.024),饲粮添加FGEL显著缓解了LPS应激肉仔鸡平均日增重(ADG)(P=0.023)和血清D-木糖的含量(P=0.011)的降低。2)与对照饲粮相比,饲粮添加FGEL显著缓解了LPS应激肉仔鸡十二指肠(P=0.011)和空肠(P=0.030)的相对重量... 相似文献
182.
Genotypic Variation for Tolerance to Transient Drought During the Reproductive Phase of Brassica rapa 下载免费PDF全文
Y. M. Guo N. C. Turner S. Chen M. N. Nelson K. H. M. Siddique W. A. Cowling 《Journal of Agronomy and Crop Science》2015,201(4):267-279
Brassica rapa L. is a genetically diverse parent species of the allotetraploid species, oilseed rape (B. napus) and a potential source of drought tolerance for B. napus. We examined the effect of a 13‐day drought stress period during the early reproductive phase, relative to a well‐watered (WW) control, on subsequent growth and development in nine accessions of B. rapa and one accession of Brassica juncea selected for their wide morphological and genetic diversity. We measured leaf water potential, stomatal conductance, water use, and leaf and bud temperatures during the stress period and aboveground dry weight of total biomass at maturity. Dry weight of seeds and reproductive tissue were not useful measures of drought tolerance due to self‐incompatibility in B. rapa. The relative total biomass (used as the measure of drought tolerance in this study) of the 10 accessions exposed to drought stress ranged from 47 % to 117 % of the WW treatment and was negatively correlated with leaf‐to‐air and bud‐to‐air temperature difference when averaged across the 13‐day stress period. Two wild‐type (B. rapa ssp. sylvestris) accessions had higher relative total and non‐reproductive biomass at maturity and cooler leaves and buds than other types. We conclude that considerable genotypic variation for drought tolerance exists in B. rapa and cooler leaves and buds during a transient drought stress in the early reproductive phase may be a useful screening tool for drought tolerance. 相似文献
183.
Y. L. Loko A. Adjatin A. Dansi R. Vodouhè A. Sanni 《Genetic Resources and Crop Evolution》2015,62(8):1181-1192
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185.
Sarasvathi P. Easwvaran Subha Bhassu Mary B. B. Maningas Rofina Y. Othman 《Journal of the World Aquaculture Society》2019,50(5):1026-1039
Myostatin (MSTN) is an interesting negative growth‐regulating gene that has been well characterized in vertebrates but scantly described in invertebrates. The current study focuses on the downregulation of the MrMSTN gene and subsequently records any histological changes for giant freshwater prawn, Macrobrachium rosenbergii (Mr). In addition, the study also deals with the MrMSTN gene's influence on other growth‐related genes, which include myosin heavy chain, dystrophin‐dystroglycoprotein complex, tropomyosin, farnesoic acid o‐methyl transferase, arginine kinase, cyclophilin, and acyl CoA desaturase. The preliminary histological analysis following MrMSTN silencing favors muscle regeneration, which supports its functional role as a negative growth regulator and its significant effect on the expression of other growth‐related genes. Overall, our results show that the MrMSTN gene could therefore be a potential target for gene manipulation aimed at enhancing the growth and muscle development of M. rosenbergii, which could be beneficial in increasing the total mass production in the postlarva phase at the hatchery level. 相似文献
186.
187.
Niora J. Fabian Michael Y. Esmail Lauren Richey Sureshkumar Muthupalani Jennifer L. Haupt Joanna Joy Sebastian E. Carrasco 《Journal of veterinary diagnostic investigation》2021,33(3):582
Cutaneous leiomyosarcomas are malignant mesenchymal tumors of smooth muscle origin and are reported occasionally in avian species. A 14-y-old male laboratory White Carneau pigeon (Columba livia) was presented for surgical excision of a cervical soft tissue mass. Ultrasonography with color flow Doppler imaging revealed multiple cavitations of mixed echogenicity within the mass and vascularization. Histologically, the dermis and subcutis were expanded by a densely cellular multinodular mass comprised of fusiform cells forming haphazardly arranged broad streams and short interwoven bundles, often surrounding blood vessels and variably sized cavitations. Neoplastic cells were strongly immunopositive for desmin and α–smooth muscle actin, and negative for pancytokeratin, S100, and von Willebrand factor. Based on histopathology and IHC findings, the cutaneous mass was diagnosed as leiomyosarcoma (LMS). The pigeon died 312 d post-operatively. Postmortem examination revealed masses infiltrating the left and right pulmonary airways and one hepatic nodule, but no regrowth at the surgical site. Histologic and IHC evaluation of the pulmonary and hepatic masses were consistent with LMS, representing metastatic foci from the primary cutaneous LMS. Our case highlights the malignant behavior and histomorphologic features of cutaneous LMS in an avian species. 相似文献
188.
Pharmacokinetic study of meropenem in healthy beagle dogs receiving intermittent hemodialysis 下载免费PDF全文
S. Y. Byun J. W. Jeong J. H. Choi K. P. Lee H. Y. Youn H. J. Maeng K. H. Song T. S. Koo K. W. Seo 《Journal of veterinary pharmacology and therapeutics》2016,39(6):560-565
Meropenem, a second carbapenem antimicrobial agent with a broad spectrum of activity, is used to treat sepsis and resistant‐bacterial infections in veterinary medicine. The objective of this study was to identify the pharmacokinetics of meropenem in dogs receiving intermittent hemodialysis (IHD) and to determine the proper dosing in renal failure patients receiving IHD. Five healthy beagle dogs were given a single i.v. dose of 24 mg/kg of meropenem and received IHD. The blood flow rate, dialysate flow, and ultrafiltration rate were maintained at 40 mL/min, 300 mL/min, and 40 mL/h, respectively. Blood samples were collected for 24 h from the jugular vein and from the extracorporeal arterial and venous line. Urine samples and dialysate were also collected. The concentrations of meropenem were assayed using HPLC/MS/MS determination. The peak plasma concentration was 116 ± 37 μg/mL at 15 min. The systemic clearance was 347 ± 117 mL/h/kg, and the steady‐state volume of distribution was 223 ± 67 mL/kg. Dialysis clearance was 71.1 ± 34.3 mL/h/kg, and the extraction ratio by hemodialysis was 0.455 ± 0.150. The half‐life (T1/2) in dogs with IHD decreased compared with those without IHD, and the reduction in T1/2 was greater in renal failure patients than in normal patients. Sixty‐nine percent and 21% of the administered drug were recovered by urine and dialysate in the unchanged form, respectively. In conclusion, additional dosing of 24 mg/kg of meropenem after dialysis could be necessary according to the residual renal function of the patient based on the simulated data. 相似文献
189.
M.‐Y. Qian L. Wei D. Zhou Z.‐J. Zhang J.‐K. He Q.‐J. Zhang P. Zhu X.‐L. Xiao 《Journal of veterinary pharmacology and therapeutics》2016,39(1):84-89
Nanotechnology applications in medicine have seen a tremendous growth in the past decade and are being employed to enhance the stability and bioavailability of lipophilic substances, such as florfenicol. This study aimed to examine the pharmacokinetic properties of the formulated oil‐in‐water florfenicol‐loaded nanoemulsion (FF‐NE). FF‐NE and florfenicol control (Nuflor®) were administered to the pigs at a dose of 20 mg/kg. Nanoemulsion formulation of florfenicol was highly influenced in vivo plasma profile. The in vivo absorption study in pigs indicated that Cmax (14.54 μg/mL) was significantly higher in FF‐NE, 3.42 times higher than the marketed formulation. In comparison with the control group, the relative bioavailability of formulated nanoemulsion was up to 134.5%. Assessment of bioequivalence using log‐transformed data showed that the 90% confidence intervals (90% CI) of Cmax and AUC0–∞ were 2.48–4.60 and 1.21–1.72, respectively. 相似文献
190.
Comparative plasma and milk dispositions,faecal excretion and efficacy of per os ivermectin and pour‐on eprinomectin in horses 下载免费PDF全文
C. Gokbulut M. Ozuicli D. Aksit E. Aksoz O. Korkut M. Yalcinkaya V. Y. Cirak 《Journal of veterinary pharmacology and therapeutics》2016,39(6):584-591
The horse milk gains increasing interest as a food product for sensitive consumers, such as children with food allergies or elderly people. We investigated the plasma and milk disposition, faecal excretion and efficacy of per os ivermectin (IVM) and pour‐on eprinomectin (EPM) in horses. Ten mares were divided into two groups. The equine paste formulation of IVM and bovine pour‐on formulation of EPM were administered orally and topically at dosage of 0.2 and 0.5 mg/kg bodyweight. Blood, milk and faecal samples were analysed using high‐performance liquid chromatography. The plasma concentration and persistence of IVM were significantly greater and longer compared with those of EPM. Surprisingly, EPM displayed a much higher disposition rate into milk (AUCmilk/plasma: 0.48) than IVM (AUCmilk/plasma: 0.19). IVM exhibited significantly higher faecal excretion (AUCfaeces: 7148.54 ng·d/g) but shorter faecal persistence (MRTfaeces: 1.17 days) compared with EPM (AUCfaeces: 42.43 ng·d/g and MRTfaeces: 3.29 days). Faecal strongyle egg counts (EPG) were performed before and at weekly intervals after treatment. IVM reduced the EPG by 96–100% for up to 8 weeks, whereas the reduction in the EPM group varied from 78 to 99%. In conclusion, due to the relatively low excretion in milk, EPM and IVM may be used safely in lactating mares if their milk is used for human consumption. Nevertheless, much lower plasma and faecal availabilities of EPM could result in subtherapeutic concentrations, which may increase the risk of drug resistance in nematodes after pour‐on EPM administration compared with per os IVM. 相似文献