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181.
182.
Hagiwara Y Fujiwara S Takai H Ohno K Masuda K Furuta T Nakayama H Doi K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):349-351
Pneumocystis carinii pneumonia was diagnosed by postmortem examination of a one-year-old Cavalier King Charles Spaniel with four-week history of dyspnea. Cytologic and histologic examination of lung tissues revealed numerous P. carinii trophozoites and cysts, and P. carinii specific DNA was detected by polymerase chain reaction. The dog showed hypogammagloblinemia and extremely low levels of serum IgG. It was considered that P. carinii pneumonia in this case was associated with an immunodeficient condition which has already been reported in Miniature Dachshunds. 相似文献
183.
To determine if Escherichia coli O157:H7 is capable of residing in the gall bladder of cattle, inoculation studies were conducted with O157:H7 strain 86-24 in weaned Holstein calves. Strain 86-24 was isolated from the gall bladders of five calves 36 days after inoculation. Two other calves contained the inoculation strain in the distal colon but the organism was absent in their gall bladders. A second trial in which the calves were euthanized 15 days after inoculation found strain 86-24 in six of seven inoculated calves but only in colon and/or rumen samples. In a third trial that inoculated eight calves with a four-strain cocktail of O157:H7 strains, the gall bladders from all eight animals were positive 9 days after inoculation. The colon and rumen samples from these calves were also positive. E. coli O157:H7 isolates recovered from bile samples and subtyped by pulsed field gel electrophoresis found that three of the four inoculation strains were present in one or more of the calves. Thus, residence in the gall bladder is not restricted to a single strain. Additional evidence of the ability to localize in the gall bladder of cattle was provided by testing the bile from 150 gall bladders (five collection dates, 30 samples each) obtained at an abbatoir and the isolation of E. coli O157:H7 from four samples (2.7%). This study establishes that E. coli O157:H7 can reside transiently or permanently at a low level in the gall bladder of cattle. 相似文献
184.
AIM: To investigate the presence of surface-active phospholipid (SAPL, or surfactant) in equine tendon and tendon sheath fluid. METHODS: The left front flexor tendon and sheath were removed from five Thoroughbred horses. Phospholipid was extracted from tendon sheath fluid using Folch reagent and quantified using spectroscopy. Transmission electron microscopy (TEM) was used to observe the tendon surfaces. RESULTS: The presence of phospholipid (90.6 (SD 4.3) microg/ml) in tendon sheath fluid, plus the appearance of oligolamellar layers and lamellar bodies on the tendon surface were indicative of SAPL. CONCLUSIONS: Evidence of SAPL was found in equine tendon, and may have a similar lubricating function as reported for synovial joints. CLINICAL RELEVANCE: These findings may have important implications for normal tendon function and possible therapeutic adjuncts for tendon and tendon sheath injuries. 相似文献
185.
S Y Yacoob K Anraku T Marui T Matsuoka G Kawamura & M Vazquez Archdale 《Aquaculture Research》2001,32(3):217-222
The gustatory sensitivity of the Nile tilapia, Oreochromis niloticus L., to different amino acids was studied using an electrophysiological approach. The electrical responses were recorded from a branch of the facial nerve innervating the external taste buds of the upper lip. The relative stimulatory effectiveness (RSE) of nine amino acids and betaine were determined at a concentration of 1 mm and all of them elicited neural responses. This species responded well to the neutral, basic and acidic amino acids. The most stimulatory amino acids were L‐histidine, L‐arginine, L‐serine, L‐methionine and L‐glutamine; L‐proline and betaine were the least stimulatory. The results of this study suggest that the Nile tilapia has high external gustatory sensitivity to some amino acids as a physiological adaptation to search effectively for their sources. The effect of the pH, ranging from 4.0 to 9.0, on the RSE of three neutral amino acids and artificial pond water (APW) was also studied. The RSE increased below pH 6.0 and was relatively unaffected from 7.0 to 9.0, indicating that acidified stimulants are highly stimulatory in this species. Nile tilapia did not discriminate the pH of APW as effectively as some of the species studied earlier. 相似文献
186.
In Ethiopia vertisols cover about 10% of the total land area and is the fourth most important soil used for crop production, accounting for nearly 23% of the total arable land used for crop production. More than half of the vertisols are found in the Central Highlands of Ethiopia, with an altitude of more than 1500 m above mean sea level. The unique physical and chemical properties of these soils and the high rainfall during the main cropping season create severe surface waterlogging problems which hinder crop production activities. Severe surface waterlogging affects the growth of plants by impeding nutrient uptake and creating oxygen deficiency around the root zone. To address this crop production problem, three surface water drainage methods, namely broad bed and furrow (BBF), ditch, and flat (traditional) methods were evaluated using the water balance of the plant root zone and wheat as a test crop. The experiment was conducted at the Ginchi Research Station in the central highlands of Ethiopia over two consecutive seasons (2000 and 2001). The results showed that both the BBF and the ditch drainage methods gave about 33% and 22% more grain yield than the flat treatment, respectively. However, there were no significant differences between BBF and ditch for both grain and biomass yield during both experimental seasons. During both seasons the total water balance (ΔWr) at the root zone especially, in the months of June, July and August on all the treatments was higher than the crop water requirement (ETc) and showed no significant difference between the treatments. Thus, the results of this study indicated that the soil water in the root zone was not significantly altered by surface drainage systems and therefore implies the need of further improvement of the different surface drainage methods regarding improving the waterlogging condition and hence the productivity of the vertisols in the Central Highlands of Ethiopia. 相似文献
187.
The reuse of saline treated industrial wastewater generated by textile firms mixed with municipal domestic effluent for irrigation was used to asses its effect on the mineral content of three olive (Olea europaea L.) cultivars under greenhouse and field conditions during two complete vegetative cycles. Chemical analysis of the treated wastewater indicated that the element concentrations fall within the permissible range of irrigation water used for plants. However, little impermissible accumulation of Na and Mg higher than the recommended maximum concentration was observed. Irrigation water with six electrical conductivities (EC = 0.78, 1.0, 2.0, 3.0, 4.0 and 5.0 dS m−1 in treatments T0, T1, T2, T3, T4, T5, respectively) were compared in the greenhouse experiment. The olive trees in the field experiment were trickle irrigated with potable water and treated wastewater (average EC = 4.2 dS m−1). The results of the greenhouse experiment showed that leaf N, Cu, Mn, Fe, Pb, and Na contents increased with increasing salinity of the treated wastewater. This increase was accompanied with a decrease in K and Mg contents. Leaf Ca and Cl concentrations were not considerably affected. Ion analysis in roots indicated that the contents of P, Na, Cl, Mn, and Pb increased while K decreased as treated wastewater salinity increased. Consequently, in most cases T4 and T5 gave a highly significant increase or decrease in accumulation of the previously mentioned minerals. A considerable variation in the studied cultivars was noticed. ‘Nabali’ was considered the most tolerant cultivar for the high salinity levels of the treated wastewater; its transporting selectivity of Na from root to leaf was higher and more Na was retained in the roots. Tissue analysis of leaves indicated that the element concentrations were within the adequate levels except those of Fe in ‘Nabali’ and ‘Manzanillo’, Na in ‘Improved Nabali’ and Cu in ‘Nabali’ and ‘Manzanillo’. In view of these findings, the negligible accumulation of minerals in leaves and roots indicated that this kind of textile effluent can be used as a valid alternative for irrigation of olive orchards with continuous monitoring of mineral levels. 相似文献
188.
W.H. Abou El‐Hassan G. El‐Kassar H. Fujimaki Y. Kitamura A. Khater 《Irrigation and Drainage》2015,64(4):454-463
Preliminary studies were conducted to test new design criteria incorporated in the Sefsafa Canal (SC) with the aim of reducing water application costs without sacrificing irrigation performance. For comparison, we also studied the Meet‐Yazied Canal (MYC) (which is run without the new techniques). The cost‐reduction measures used in this study included reduction of pump discharge rates and the use of electricity instead of diesel. We found that the location of farms along the irrigation canal had little effect on pump operation hours and amount of applied water; instead, crop patterns were the most important factor in this regard. The water use index (WUI), which is the ratio of applied water to required water, was higher in the SC than in the MYC and El‐Mesk Canal (MC). Decreasing the pump discharge increased the amount of applied water to meet crop water requirements. During the winter, the WUI values of 1.11–1.16 achieved discharge rates of 70–80 l s−1 and were considered the optimal values of WUI. Further studies are required to avoid excessive application of water during the summer. The application of new techniques prompted farmers to irrigate their fields at night, thereby reducing water losses and installation and operational costs, and promoting equitable water distribution along the entire irrigation canal and throughout the command area. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
189.
The genetic relationship between popping expansion volume and two yield components in popcorn using unconditional and conditional QTL analysis 总被引：2，自引：0，他引：2
Y. L. Li Y. B. Dong D. Q. Cui Y. Z. Wang Y. Y. Liu M. G. Wei X. H. Li 《Euphytica》2008,162(3):345-351
Popping expansion volume (PEV) is the most important quality trait in popcorn, while its germplasm is inferior to normal dent/flint
corn in yield. In this study, 259 F2:3 families, developed from the cross between a dent corn inbred Dan232 and a popcorn inbred N04, were evaluated for their PEV,
grain weight per plant (GWP) and 100-grain weight (100 GW) in two environments. The genetic relationship between PEV and GWP,
and 100 GW on individual gene loci were evaluated using unconditional and conditional QTL mapping methods. In total, five,
one and three unconditional QTL were identified for PEV, GWP, and 100 GW, respectively. The positive alleles of all QTL for
PEV were from N04, while positive alleles of all QTL for GWP and 100 GW were from Dan232. In conditional mapping, one and
two QTL failed to be detected, and all four additional QTL were detected. Nevertheless, three QTL were identified, which controlled
PEV independently from GWP/100 GW. They seemed to be potential candidates in popcorn breeding to increase PEV without decreasing
GWP/100 GW. The results suggested that for significantly correlated traits, the conditional QTL mapping method could be used
to dissect the genetic interrelationship between traits at the level of individual QTL, as well as reveal additional QTL that
were undetectable by unconditional mapping. 相似文献
190.
The extent of genetic variation in seed and seedling traits of Trigonobalanus doichangensis, a rare and endangered tree in southwest China, was evaluated at the population level. Seeds were collected from four natural populations in the south and southwest Yunnan province, China. Significant differences (P < 0.05) between populations were observed in seed length, seed weight and seedling morphological characters. The analyses revealed that none of the seed and seedling traits had significant correlations with the geo-climatic variables of the populations. Among seed and seedling traits, significant correlations (P < 0.05) were discovered among seed length, seed weight and height of 30-day-old seedlings. Significant correlation (r = 1.00, P < 0.01) occurred also between the height and root collar diameter of 4-year-old seedlings. With the exception of seed width and seed germination percentage, 51–96% of total variation in other seed and seedling related traits was attributed to population effect, as determined by the broad sense heritability. In conclusion, these observations on variation in seed and seedling traits should be a reference point, when considering seed collection of this species for ex situ conservation and species restoration. 相似文献