Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.     

控制sch单养雄蚕品种雌雄蚕孵出比例的催青处理因素研究

研究结果表明 :在RH 6 0 %状态下 ,控制性比的温度和下限时间负相关达到显著水平 ;控制性比的高温处理适于干燥状态下进行 ;处理后雌蚕孵化整齐度明显低于雄蚕孵化整齐度 ,雌雄蚕孵化整齐度均随处理温度的升高而下降 ,尤以雌蚕更为明显。由此提出了在RH 6 0 %状态下sch单养雄蚕品种控制性比的起点温度及温度、时间范围。认为高温催青用下限温度配合 1日收蚁法效果好 ,高温处理以后的胚胎适于在RH 80 %～ 85 %的湿度中保护     

几种绢丝昆虫遗传多样性的RAPD研究

选用重复性较好的 10个引物对 6种绢丝昆虫 2 3个个体进行扩增 ,共得到 2 45个RAPD标记。RAPD分析结果表明 :家蚕及野桑蚕种内各品种材料的遗传距离较大 ,天蚕、蓖麻蚕及柞蚕较小 ;家蚕与野桑蚕两者之间遗传距离小 ,反映出家蚕和野桑蚕亲缘关系较近 ;而天蚕、蓖麻蚕和柞蚕三者之间以及分别与家蚕和野桑蚕之间的遗传距离较大 ,亲缘关系远。聚类分析的结果与之相同。并且对家蚕的起源及绢丝昆虫遗传多样性的保护利用做了探讨。     

影响奶牛初乳产量的因素探讨

奶牛初乳是指母牛分娩后7天内所分泌的乳汁。初乳中不仅含丰富的营养物质，而且含有大量的免疫因子，因此具有促进生长发育、改善胃肠道功能、增进免疫调节、延缓衰老、抵御疾病等作用，是人类功能性保健食品的重要基料。初乳及其制品被誉为天然、完美、完全、廉价的“21世纪保健食品”。我国有奶牛和改良牛426.5万头(1998)，初乳资源十分丰富，但迄今对初乳产量及其影响因素尚未见有报道，于是对此问题我们在新疆乌鲁木齐五一奶牛场进行了初步探讨。1材料收集整理原始产奶记录和产犊记录取自该场1965年至1997年资料，全都为舍饲中国荷斯坦…     

一种估测亚麻出麻率的简便方法

统计分析显示亚麻茎中段的出麻率与单株出麻率相关极显著，可以在育种的单株选择过程中，利用茎中段的出麻率来快速估算单株出麻率，以减轻工作量，提高工作效率。     

高温条件下超级稻对灌水处理的响应及原因分析

为预防高温天气对超级稻的影响,通过灌水深度和灌水时期试验,研究了超级稻产量和结实率对不同灌水深度和不同灌水时期的响应及原因。结果表明:不同灌水深度处理产量及结实率存在显著差异,灌水能提高产量和结实率,灌深水的效果比灌浅水好。不同灌水时期处理,灌水提高产量和结实率的幅度不同,效果最好的是在幼穗分化五期￣齐穗。不同灌水处理影响产量和结实率的原因是灌水能降低田间温度、减少日平均温度≥30℃或最高温度≥35℃天数以及灌水时超级稻发育对温度敏感程度不同。     

桑蚕卵中酯酶A_4的研究

取产后两天内25℃保存的桑蚕卵为材料,精制酯酶A_4。将滞育卵中精制的酶液置5℃下冷藏,冷藏初期酶活性极低,大致在12～13日达最大值。以后迅速降低,体外保存的温度条件与酯酶A_4活性有关,较高的保存温度能促使酶活性峰提早出现:而低温保护能延缓酶的活化进程。酯酶A_4精制过程中,通过Sephadex G-25凝胶过滤,将大部分物质分段与酶分离排除,在5℃条件下,酶活性峰出现以前的任何时候加入排除分段物质,都可以停止酶的活性化,只有当重新除去所加的排除分段物质时,酶的活性化进程才得以继续。     

红曲霉色素的提取

本实验确立了一种新的红曲霉色素提取方法:以红曲米为原料,乙醇为提取剂,得到的提取液再以正己烷提取分离。结果表明,此法所得的红曲霉色素是水溶性的,宜于用作食品添加剂。此外,还对影响提取的因素进行了探讨,并提供了最佳提取方案。