紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应

采用紫外诱变(波长260nm，功率15w)对一株产α-乙酰乳酸脱羧酶的枯草芽孢杆菌（Bacillus subtilis）BS059菌株进行诱变处理。结果表明，在紫外辐照时间为50s时，诱变效果较好。从正突变菌株中反复筛选，得到两株产酶量较高的菌株BS059-15和BS059-22，比原出发菌株的酶活分别提高了107.62%和162.25%。经过连续传代试验，证明其遗传性状稳定，为可遗传变异。     

利用SSR标记构建贵州山区玉米杂交种的指纹库

对贵州山区目前在生产上正在使用的西山系列玉米杂交种12份进行了SSR分析。从60对引物中筛选到稳定性好、多态性丰富的8对引物作为核心引物。用这8对引物的指纹组合构建了贵州山区西山系列玉米杂交种的DNA指纹数据库。结果表明,利用SSR技术进行玉米杂交种真实性的鉴定是可行、有效的。     

Nonlinear Characteristic Modeling of Servo Value on Hydraulic Bending Roll System''s Dynamic Characteristic

Nonlinear characteristic of servo value is the important factor influencing hydraulic system, the model of electro-hydraulic servo pressure control system is designed. In this model, flux-equation of servo valve is specially applied and some non-linear characteristics are considered. Furthermore, SIMULINK toolbox of MATLAB language software is applied to solve mathematic model and simulation curves of some variables are obtained. The calculation results are in good agreement with the experimental results.     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

Third-party Logistics Subcontract Design Based on Bilevel Programming

The research theme of this paper is about how third-party logistics provider design a kind of effective incentive mechanism which can not only avoid the moral hazard behavior of the subcontractor , but also promote subcontractor to raise the serving performance constantly, until obtaining the best TPL performance .Through building bilevel programming model where constructing the Nash equilibrium model this paper, the anthors assume subcontract decision variable as endogenetic variable first, and then analyze the optimal solution with the restraint of Nash equilibrium at the time of decision variable as exogenous variable, push the subcontract relationship research at the principal-agent level into the lay that principal-agent combining with whole contract performance optimal decision.     

Inspiration to Reuse of Old Industry Building with SOHO

This paper analyzes the SOHO and the reuse of old industry building, and deeply explores the creation and application of SOHO mode in the reuse of old industry building, furthermore, it points out the four inspirations to the reuse of old industry building, at the end, a general judgment of the development logic of SOHO is given.     

Analysis of Project Audit''s Function and Civil Action

Audit takes a unique status and has obvious function in financial surveillance system.The Audit is used to superintend the factuality,validity and virtue of fiscal and financial income and expenses.In the renewed China Audit law the functions and status of official audit have been intensified,and the qualification and status of Audit department as executing office is confirmed.This paper analyzed the official construction project audit's function and legal status.     

Preparation of Electrochromic and Transparent Film of Polyaniline

The preparation method of transparent film of electrochromism polyaniline on the glass supporter is studied by a dipping polymerization. The electric and optic properities of the resulting film are measured as a function of oxidant,dopant,reaction time and temperature.The optimum technology term is obtained in the normal atmospheric temperature.The experimental result is testified by fuzzy set theory.     

The Public Participation in the Urban Planning

The policy of public participation in urban planning was first practiced in Europe in the 1960s. After 40 years of development, the policy has become the essential step of city planning in Western countries where as is just at the elementary step in China. For the purpose of knowing the situation about the policy performing in local cities and discussing the feasibility of public participation in urban planning, several kinds of investigation have been done. From the investigation, it is found that although the public participation has already been the important step of urban planning in some city during just several years,there are still many problems. This paper shows a panorama view of this issue. Th describe the future of public participation in e authors will offer some solutions according to the problems and the urban planning.