牛羊养殖中前胃疾病鉴别诊断方法

牛羊都是反刍动物,它们拥有4个胃,分别是瘤胃、网胃、瓣胃和真胃。前3个胃统称为前胃,在前胃中有大量的可以分解和消化饲料中粗纤维的细菌,是牛羊以粗饲料作为主要食物的原因之一。很多牛羊非常容易出现前胃疾病,饲养人员要掌握牛羊前胃疾病的预防办法和治疗措施。     

雏鹅死亡原因及防治措施

鹅是草食水禽类动物,具有独特的生物学特征和生理特征。雏鹅是指21日龄内的小鹅,该阶段鹅绒毛稀少,身体较小,体温调节能力不足,消化机能不健全,对外界环境的适应能力和抗病能力普遍较弱,一旦饲养管理不当,没有进行妥善的疫苗免疫接种,易造成病原的传播流行,引发死亡。在雏鹅阶段,应充分掌握引发死亡的具体原因,并从养殖管理等诸多环节入手,为雏鹅营造健康舒适的生长环境,提高群体的身体抵抗能力,降低发病率和死亡率,确保养殖安全。该文主要结合一个鹅场的养殖案例,论述引起雏鹅死亡的原因,并提出相应的防治措施。     

鸡呼吸道疾病鉴别诊断与防治

呼吸道疾病是目前威胁鸡养殖产业健康发展的一类重大动物疫病,呼吸道疾病的病原种类多种多样,且造成的临床症状大致相同,诊断难度较大,如果没有进行妥善有效的鉴别、诊断,易造成整体的治疗方案缺乏针对性,难以在短时间内控制病情,错过最佳的防控时期。鸡呼吸道疾病常常混合感染并发感染,2种以上的病原相互叠加表现的临床症状复杂,诊断难度更大。该文主要论述鸡呼吸道疾病的鉴别诊断和防治措施。     

Necrotic enteritis challenge regulates peroxisome proliferator-1 activated receptors signaling and β-oxidation pathways in broiler chickens

Necrotic enteritis (NE) is an important enteric disease in poultry and has become a major concern in poultry production in the post-antibiotic era. The infection with NE can damage the intestinal mucosa of the birds leading to impaired health and, thus, productivity. To gain a better understanding of how NE impacts the gut function of infected broilers, global mRNA sequencing (RNA-seq) was performed in the jejunum tissue of NE challenged and non-challenged broilers to identify the pathways and genes affected by this disease. Briefly, to induce NE, birds in the challenge group were inoculated with 1 mL of Eimeria species on day 9 followed by 1 mL of approximately 10⁸ CFU/mL of a NetB producing Clostridium perfringens on days 14 and 15. On day 16, 2 birds in each treatment were randomly selected and euthanized and the whole intestinal tract was evaluated for lesion scores. Duodenum tissue samples from one of the euthanized birds of each replicate (n = 4) was used for histology, and the jejunum tissue for RNA extraction. RNA-seq analysis was performed with an Illumina RNA HiSeq 2000 sequencer. The differentially expressed genes (DEG) were identified and functional analysis was performed in DAVID to find protein–protein interactions (PPI). At a false discovery rate threshold <0.05, a total of 377 DEG (207 upregulated and 170 downregulated) DEG were identified. Pathway enrichment analysis revealed that DEG were considerably enriched in peroxisome proliferator-activated receptors (PPAR) signaling (P < 0.01) and β-oxidation pathways (P < 0.05). The DEG were mostly related to fatty acid metabolism and degradation (cluster of differentiation 36 [CD36], acyl-CoA synthetase bubblegum family member-1 [ACSBG1], fatty acid-binding protein-1 and -2 [FABP1] and [FABP2]; and acyl-coenzyme A synthetase-1 [ACSL1]), bile acid production and transportation (acyl-CoA oxidase-2 [ACOX2], apical sodium–bile acid transporter [ASBT]) and essential genes in the immune system (interferon-, [IFN-γ], LCK proto-oncogene, Src family tyrosine kinase [LCK], zeta chain of T cell receptor associated protein kinase 70 kDa [ZAP70], and aconitate decarboxylase 1 [ACOD1]). Our data revealed that pathways related to fatty acid digestion were significantly compromised which thereby could have affected metabolic and immune responses in NE infected birds.     

Effects of soybean raffinose on growth performance,digestibility,humoral immunity and intestinal morphology of growing pigs

There are appreciable does of raffinose in soybean, but the impacts of raffinose on pigs are poorly investigated. We used 2 experiments to investigate the influence of soybean raffinose on growth performance, digestibility, humoral immunity and intestinal morphology of growing pigs. In Exp. 1, a total of 30 crossbred (Duroc × Landrace × Yorkshire) barrows (21.93 ± 0.43 kg) were randomly divided into 3 groups, and were fed with the control diet, the control diets supplemented with 0.2% and 0.5% raffinose, respectively, for 21 d. Results showed that the addition of 0.2% or 0.5% raffinose reduced (P < 0.05) average daily feed intake (ADFI), average daily gain (ADG) and nutrient digestibility, and dietary 0.5% raffinose increased the ratio of feed to gain (P < 0.05). For serum indexes, dietary 0.5% raffinose decreased growth hormone and increased glucagon-like peptide-2, immunoglobulin G, tumor necrosis factor-α (TNF-α) and interleukin-6 concentration (P < 0.05). In Exp. 2, a total of 24 crossbred barrows (38.41 ± 0.45 kg) were randomly divided into 3 groups, and were fed with the control diet (ad libitum), the raffinose diet (0.5% raffinose, ad libitum), and the control diet in the same amount as the raffinose group (feed-pair group) for 14 d, respectively. Compared with the control diet, dietary 0.5% raffinose decreased ADFI (P < 0.05). Intriguingly, the raffinose group had lower ADG than the feed-pair group, lower nutrient digestibility, lower amylase activity in duodenum, lower amylase, lipase and trypsin activities in jejunum and higher TNF-α concentration in serum compared with the other 2 groups, and a higher ratio of villus height to crypt depth compared with the control group (P < 0.05). These results showed that soybean raffinose could reduce feed voluntary intake and body gain while improving intestinal morphology without a significant negative influence on immunity. Taken together, dietary raffinose could decrease growth performance by reducing both feed intake and nutrient digestibility while inducing humoral immune response of growing pigs.     

萝卜各级分枝种子质量比较

对萝卜各级分枝种子质量检验表明，萝卜各级分枝种子质量不一，以主轴顶端和基部新发枝种子质量最差，3级分枝种子质量最好，2分枝和1级分枝稍次。各分枝种子播种后，幼苗质量与种子室内检验质量结果相统一，1级、2级、分枝种子收获量最多，分别为种子收获总量的45％和38％。     

灌溉设备产品信息与报价系统的开发

本文介绍了开发灌溉设备产品信息与报价系统的必要性及功能 ,较系统地阐述了该软件系统的实现思路 ,并对其应用前景作了初步预测     

级进模自动排样冲切刃口设计

针对级进模难于实现自动化的排样问题,通过对不满足悬臂和凹槽强度检验要求的多义线进行分析和对外形轮廓的分解与重组,对级进冲裁模排样系统中冲裁零件的冲切刃口进行了分析与设计;同时为保证凹模壁厚达到强度要求,在分析冲切刃口的干涉关系的基础上,根据分析结果设计了程序,该程序能够自动重新生成级进模各工位的冲切刃口和外形,以实现级进模的自动工序排样。     

Object‐Based Mapping of Karst Rocky Desertification using a Support Vector Machine

Accurate and cost‐effective mapping of karst rocky desertification (KRD) is still a challenge at the regional and national scale. Visual interpretation has been utilised in the majority of studies, while an automated method based on pixel data has been investigated repeatedly. An object‐based method coupling with support vector machine (SVM) was developed and tested using Enhanced Thematic Mapper Plus (ETM+) images from three selected counties (Liujiang, Changshun and Zhenyuan) with different karst landscapes in SW China. The method supports a strategy of defining a mapping unit. It combined ETM+ images and ancillary data including elevation, slope and Normalized Difference Vegetation Index images. A sequence of scale parameters estimation, image segmentation, training data sampling, SVM parameters tuning and object classification was performed to achieve the mapping. A quantitative and semi‐automated approach was used to estimate scale parameters for segmenting an object at an optimal scale. We calculated the sum of area‐weighted standard deviation (WS), rate of change for WS, local variance (LV) and rate of change for LV at each scale level, and the threshold of the aforementioned index that indicated the optimal segment level and merge level. The KRD classification results had overall accuracies of 85·50, 84·00 and 84·86 per cent for Liujiang, Changshun and Zhenyuan, respectively, and kappa coefficients are up to 0·8062, 0·7917 and 0·8083, respectively. This approach mapped six classes of KRD and offered a visually appealing presentation. Moreover, it proposed a conceptual and size‐variable object from the classification standard of KRD. The results demonstrate that the application of our method provides an efficient approach for the mapping of KRD. Copyright © 2012 John Wiley & Sons, Ltd.     

豫西丘陵地区果牧结合立体生态园设计的探讨

根据豫西丘陵地区生态、经济、社会条件以及目前农牧业生产中存在的突出问题，提出果牧结合立体生态园建设是该地区生态农业发展的重要途径。并在调查和初步研究基础上设计了“畜（禽）—果”、“畜（禽）—沼—果”等果牧结合模式，分析了生态园建设研究中要探讨的具体内容。