2005-2020年转Bt基因棉花抗虫性变化及其与产量性状的相关性分析

为了给转Bt基因棉花在科研和生产上多世代可持续利用提供依据,以转Bt基因棉花品种银山8号为试验材料,从2005年开始,连续16年进行跟踪研究。结果表明,随种植世代的增加,生物测定银山8号第2～4代棉铃虫幼虫校正死亡率、铃期叶片和铃期小铃杀虫蛋白表达量均有不同程度的线性上升趋势,同时皮棉产量线性回归也呈增长趋势;苗期叶片、蕾期叶片和蕾期小蕾杀虫蛋白表达量呈线性下降趋势。相关性分析表明,抗虫棉的抗虫性与皮棉产量间均呈正相关,株铃数和衣分是构成皮棉产量的重要因子,与皮棉产量的相关性分别达到极显著和显著水平。外源Bt基因转入棉花后能稳定遗传给后代,通过卡那霉素鉴定和系统选择可以持续保持转Bt基因棉花多世代的抗虫性,甚至通过提纯复壮逐年优选,可实现转Bt基因抗虫棉特定生育时期或部分棉花器官抗虫性的提高。     

钩藤碱提取工艺研究

对钩藤碱提取工艺进行研究,确定用碱化苯提法提取钩藤碱,经处理后可得到高纯度钩藤碱．该工艺简单可行,适于大规模生产．     

不同制粒温度对植酸酶活性的影响及畜禽饲养对比试验研究

试验将含有制粒专用植酸酶制剂的日粮样本在4种温度条件下进行制粒．制粒后的饲料应用植酸酶国标法进行酶活测定,结果表明：制粒专用植酸酶在调制温度70℃左右、蒸汽压力不高的情况下酶活基本未丧失,性能比较理想：当制粒温度达到80℃以上时,酶活明显降低,但总体保持在500U／kg以上．制粒用植酸酶仍具有较好的耐热性能。经过动物饲养试验表明．肉鸡和育肥猪饲养对照组和处理组之间的平均日增重和料肉比差异都不显著（p〉0．01）,这说明在添加制粒专用植酸酶后．对磷酸氢钙的量进行调整并不会影响肉鸡和育肥猪的生长性能．且同样能达到普通植酸酶在粉状饲料中的应用效果。     

浅谈巫溪县夏秋蚕生产潜力

栽桑养蚕是巫溪县农村的传统产业,是蚕区农民致富奔小康的骨干项目。自上世纪80年代初,县委、县府就已将栽桑养蚕作为农村特色产业大力发展,1988年首次突破万担茧大关,发种量达到2万张,蚕农收入逾500万元;丝厂绸厂从无到有逐渐完善,农、工、贸产业链已基本形成。全县12个乡镇、88个养蚕村、2万余农户,8万多人口从事栽桑养蚕;在地桑0.8亿株、年养蚕4万余张、产茧1 000t、收入1 600万元以上。2006年《重庆市百万担优质蚕茧产业化工程“十一五”发展规划》把巫溪等渝东南10个区市县列为重庆市生产优质蚕茧最适宜区域重点发展,规划我县在“十一五”期内达到2 500t优质蚕茧的规模。要达到这一目标,摆在我们面前的任务是如何深挖潜力,努力提高蚕茧单产,特别是努力提高夏秋蚕单张产量,为整体提高全县养蚕的科技水平,作出自己新的贡献。1基本概况1.1自然概况我县位于渝陕鄂交汇处,东经108°44′~109°58′,北纬31°14′~31°44′之间,最低海拔135m,最高海拔2 797m(阴条岭山为重庆第一峰),由于海拔差异大,形成了独特的立体气候,素有“一山兼四季、十里不同天”的美称。终年气候温和,雨量充沛,日照时间长...     

幼犬肘头度下黏液囊炎的诊断1例

2007年6月24日,我基地1头牧羊犬产下5头仔犬,仔犬健康良好.9月12日进行健康检查时,发现其中1头小犬(公,3月龄)双侧肘头部有明显的肿胀,触诊有温热感,波动明显,大如鸡蛋.小犬精神状态良好,体温、吃喝正常,未出现其他异常情况.     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

蜂花粉抗化学性肝损伤研究进展

近年来研究发现蜂花粉具有显著的保肝功效,国内外学者已对此开展了广泛而深入的研究。本文就蜂花粉及活性成分对化学性肝损伤(四氯化碳肝损伤、酒精性肝损伤、氨基半乳糖诱导肝损伤)的作用效果和作用机理等方面的研究进展进行了综述,并对其研究方向和前景进行展望。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

锥—46抑制[^3H]次黄嘌呤掺入伊氏锥虫核酸的研究

用液体闪烁计数法对锥—46的抗锥虫作用的机理做了初步研究。发现T-46与Berenil很相似,均可显著抑制[~3H]次黄嘌呤掺入伊氏锥虫,抑制作用与浓度及时间呈正相关。T-46和Berenil对DNA合成的IC_(60)分别为1.33和1.73μg·ml~(-1)。本实验还提示T-46抗锥虫作用可能与损伤DNA模板有关。     

西安地区鸵鸟养殖现状调查

简述了国内外鸵鸟养殖概况 ,通过对西安地区 5个公司的鸵鸟养殖场中的饲养管理、繁育、疾病防治及产品开发等的调查和分析 ,提出了西安地区在鸵鸟养殖方面存在的问题及我国鸵鸟养殖业的发展对策