拖拉机自动转向系统设计及仿真

以TN654拖拉机为平台,基于原车液压转向系统,设计一套比例方向电磁阀组和电控单元(ECU),构建了能响应程序控制的自动转向系统。通过建立阀控缸动力系统数学模型,利用Mat Lab对该系统PD控制性能进行仿真分析。仿真结果显示:系统最大迟滞0.24、1.8s内均能达到平衡点,表明系统具有良好的控制精度和响应性,符合拖拉机自动驾驶作业需求。     

整秆式甘蔗收获机内物流排杂高速摄影分析

为了研究整秆式甘蔗收获机内物流排杂运动机理,研制了甘蔗收获机内物流排杂试验台,并利用高速摄像机拍摄了甘蔗与蔗叶在物流通道内的运动全过程,并对图像进行分析。结果表明,甘蔗在物流运动过程中发生弹跳、扭转和弯曲变形,在剥叶运动过程中发生断尾,并且通过排杂装置将蔗叶与甘蔗分离后排出。通过建立甘蔗收获机内各部件元件与甘蔗、蔗叶的简化力学模型,结合甘蔗和蔗叶的力学特性分析了甘蔗和蔗叶在物流通道内的运动机理。甘蔗在物流运动中主要受到的作用力为各部件对甘蔗的轴向力、垂直于甘蔗运动方向的切向力和摩擦力。     

Nanoemulsion formulation of florfenicol improves bioavailability in pigs

Nanotechnology applications in medicine have seen a tremendous growth in the past decade and are being employed to enhance the stability and bioavailability of lipophilic substances, such as florfenicol. This study aimed to examine the pharmacokinetic properties of the formulated oil‐in‐water florfenicol‐loaded nanoemulsion (FF‐NE). FF‐NE and florfenicol control (Nuflor^®) were administered to the pigs at a dose of 20 mg/kg. Nanoemulsion formulation of florfenicol was highly influenced in vivo plasma profile. The in vivo absorption study in pigs indicated that C_max (14.54 μg/mL) was significantly higher in FF‐NE, 3.42 times higher than the marketed formulation. In comparison with the control group, the relative bioavailability of formulated nanoemulsion was up to 134.5%. Assessment of bioequivalence using log‐transformed data showed that the 90% confidence intervals (90% CI) of C_max and AUC_0–∞ were 2.48–4.60 and 1.21–1.72, respectively.     

The screening and evaluation of herbs and identification of herbal combinations with anti-viral effects on Newcastle disease virus

1. A total of 25 “heat-clearing and detoxifying” herbs used in Chinese medicine were investigated for their cytopathic effects on the growth of Newcastle Disease virus (NDV) in a chicken fibroblast cell line.

2. The 5 herbs with the highest virus inhibitory effects were Herba agastaches, Flos chrysanthemi indici, Rhizoma anemarrhenae, Astragalus root and Baikal skullcap root and these were used in herbal formulations. Anti-NDV activities of 4 formulations were tested on the growth of NDV in the DF-1 fibroblast cell line.

3. Formulation II, containing Baikal skullcap root, Astragalus root, Anemarrhena rhizome (1:1:2) and formulation IV containing Anemarrhena rhizome, Astragalus root and Flos chrysanthemi indici (1:1:1), which had strong anti-NDV activity in vitro, were used to determine the in vivo inhibitory effects of NDV-infection in chickens. After treatment with the two formulations serum IgY titres against NDV were improved, and morbidity was reduced in the NDV-infected chickens.

4. The results suggest that the components in formulations II and IV acted synergistically to improve resistance to Newcastle disease and provide a basis for the developing an anti-NDV herbal medicine.

     

HSP90 gene expression induced by aspirin is associated with damage remission in a chicken myocardial cell culture exposed to heat stress

1. To understand the potential protection of heat shock protein 90 (HSP90) induced by aspirin against heat stress damage in chicken myocardial cells, enzyme activities related to stress damage, cytopathological changes, the expression and distribution of HSP90, and HSP90 mRNA levels in the myocardial cells exposed to heat stress (42°C) for different durations with or without aspirin administration (1 mg/ml, 2 h prior) in vitro were investigated.

2. Significant increase of enzyme levels in the supernatant of heat-stressed myocardial cells and cellular lesions characterised by acute degeneration, karyopyknosis and karyorrhexis were observed, compared to non-treated cells. However, the lesions of cells treated with aspirin were milder, characterised by earlier recovery of enzyme levels to the control levels and no obvious heat stress-related cellular necrosis.

3. Stronger positive signals in the cytoplasm and longer retention of HSP90 signal in nuclei were observed in aspirin-treated myocardial cells than those of only heat-stressed cells. HSP90 level in the aspirin-treated myocardial cells was 11.1-fold higher than that in non-treated cells, and remained at a high level at the early stage of heat stress, whereas it was just 4.1-fold higher in only heat-stressed cells and returned rapidly to a low level.

4. Overexpression of HSP90 mRNA in aspirin-treated cells was observed throughout the experiment, whereas HSP90 mRNA decreased significantly only in heat-stressed cells.

5. The early higher HSP90 expression induced by aspirin during heat stress was accompanied by decreased heat stress damage, suggesting that aspirin might play an important role in preventing myocardial cells from heat stress damage in vitro.

     

Free choice feeding of whole grains in meat-type pigeons: 1. effect on performance,carcass traits and organ development

1. The effects of 5 different feeding systems on the performance, carcass traits and organ development were studied in pigeon squabs.

2. The 5 treatments were (1) whole grains of maize, pea and wheat plus concentrate feed; (2)whole grains of maize and wheat plus concentrate feed (CWC); (3) whole grains of maize and pea plus concentrate feed; (4)whole grain of maize plus concentrate feed (CC); and (5) compound feed (CF).

3. Feed intake of parent pigeons increased significantly from 0 to 21 d and it was higher in the CF treatment. Body weight of squabs in the CWC treatment was the highest among the 5 treatments in 4 weeks. Body weight losses of parental pigeons during the rearing period were not significantly different among the 5 treatments.

4. Protein intake in CC and CWC treatments was lower than that of the other three treatments. The CWC treatment had the highest daily weight gain and the lowest feed conversion ratio. Treatments were statistically similar in the relative weight of carcass, breast and thigh. CF had the lower relative weight of abdominal fat.

5. Relative weight of gizzard in the CF treatment was significantly lower than that of CWC.

6. It was concluded that the application of free choice feeding of whole grains of maize and wheat plus concentrate feed increased the body weight of 28-d-old pigeon squabs and decreased the feed conversion rate of parent pigeons. This feeding strategy could be commercially interesting in meat-type pigeon production.

     

水分胁迫对幼龄仁用杏叶片光合特性的影响

以2年生仁用杏为试材,采用盆栽方法,以正常供水(土壤含水量为田间最大持水量的75%)为对照,设置了轻度水分胁迫(55%)、中度水分胁迫(45%)和重度水分胁迫(30%)3个处理,开展了水分胁迫条件下仁用杏叶片光合色素、光合指标及叶绿素荧光参数等光合特性研究。结果表明:水分胁迫不同程度降低了类胡萝卜素含量、叶绿素a、b含量、净光合速率(Pn)、蒸腾速率(Tr)、水分利用效率(WUE)、最大荧光产量(Fm)、最大光化学效率(Fv/Fm)和PSⅡ潜在活性,且下降幅度随着胁迫时间延长而增大;轻度水分胁迫下降幅度很小,中度和重度水分胁迫下降幅度较大。     

十种野生地被植物的光合特性研究

以东方草莓(Fragaria orientalis)、斑叶堇菜(Viola variegata)、匍枝委陵菜(Potentilla flagellaris)、鹅绒委陵菜(Potentilla anserina)、野罂粟(Papaver nudicaule)、蓝花棘豆(Oxytropis coerulea)、求米草(Oplismenus undulatifolius)、大叶铁线莲(Clematis heracleifolia)、歪头菜(Vicia unijuga)、秃疮花(Dicranostigma leptopodum)等10种野生地被植物为试材,利用LI-6400光合仪研究了净光合速率日变化以及对光合有效辐射和二氧化碳的响应。结果表明:东方草莓及秃疮花的光合速率日变化为单峰曲线;野罂粟、斑叶堇菜、东方草莓、匍枝委陵菜、鹅绒委陵菜、蓝花棘豆、求米草、大叶铁线莲等8种植物光合速率日变化为双峰曲线,出现明显的"午休"现象;求米草的光补偿点(LCP)和二氧化碳补偿点(CCP)最低,分别为(9.64±0.525)μmol·m~(-2)·s~(-1)和(67.72±4.567)μmol·m~(-2)·s~(-1),是典型的阴生植物;蓝花棘豆的LCP最高,为(74.21±1.065)μmol·m~(-2)·s~(-1),秃疮花的CCP最高,为(107.94±9.345)μmol·m~(-2)·s~(-1),2种植物表现出较强的喜阳性。     

利用香菇菌糠栽培罗勒初探

以香菇菌糠为试材,采用不同比例的香菇菌糠和花园土、砂土的混合基质种植罗勒,测定其发芽率、形态指标以及生理指标,研究了香菇菌糠对罗勒幼苗生长的影响。结果表明:适宜种植罗勒的菌糠配方为花园土、菌糠、砂子=6∶1∶1;不同比例的菌糠配方对罗勒的发芽率有一定影响,随着菌糠比例的不断增加,罗勒的发芽率也不断提高,发芽时间也随菌糠的增加有一定缩短;该试验为菌糠的循环利用开辟了一条新的途径。     

外源乙烯和1-甲基环丙烯处理对采后胡萝卜品质的影响

以胡萝卜为试材,采用乙烯及乙烯受体抑制剂(1-甲基环丙烯)处理,通过对贮藏60d后胡萝卜的品质劣变率(长须根率、腐烂指数、出薹率)、外观指数、可溶性糖含量及多酚氧化酶活性进行测定,研究了1-MCP对胡萝卜采后贮藏品质的影响。结果表明:1-MCP结合手洗贮藏可抑制胡萝卜贮藏期间的品质劣变率、抑制可溶性糖含量的损失、保持较高的外观指数及抑制多酚氧化酶(PPO)活性。手洗后贮藏则可有效改善胡萝卜贮藏期间的表皮褐变问题。