类鼻疽的研究进展

本文就类鼻疽(melioidosis)的流行病学,诊断方法,免疫机理,发病机理及治疗方法 进行了综述。为重视该病的研究提出了建议。     

春丰50苦瓜的选育

以曼谷青皮、碧绿1号和钦州大肉苦瓜为育种材料,采用双父本混合花粉杂交和后代系谱选择育成优良自交系,再经配合力测定选出优良一代杂种春丰50。该品种生长势强,果实粗棒形,纵径26.3cm,横径6.45cm,皮色翠绿油亮,一般单果质量420g左右。平均每667m~2产量3 500kg。     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.     

细胞骨架解聚药物对小麦与叶锈菌互作诱发的细胞过敏性反应的影响

 小麦(Triticum aestivum)品种洛夫林10和叶锈菌小种366组成不亲和组合,小麦叶片发生过敏性坏死反应(HR)是小麦抵抗叶锈菌侵染的重要因素。在接种前给小麦叶片分别预注射微管解聚药物磺草硝(oryzalin)和微丝解聚药物细胞松弛素D (cytochalasin D,CD),结果表明2种药物注射使得寄主因叶锈菌侵染诱导的细胞过敏性坏死数目明显减少,并且注射药物的浓度越大,寄主细胞发生HR的数量越少。说明肌动蛋白和微管蛋白的聚合状态是诱发小麦叶片发生HR防卫反应所必需的,细胞骨架在小麦抵抗叶锈菌侵染过程中可能起着重要作用。     

大白菜软腐病菌游动性突变体在大白菜体内增殖扩展研究

 用培养皿滤纸吸附测定法和不伤根土壤拌菌处理及针刺接种法,测定了大白菜软腐病菌游动性突变体进入大白菜体内、并在其中侵染定殖和扩展的特性。结果表明,游动性丧失和增强的突变体都可以通过种子萌发和主动接触进入大白菜体内、并可以在体内有短期的繁殖,但菌量远低于野生菌。大白菜叶片接种实验说明,这两种突变体也都可以进行短距离扩展,但扩展距离和菌的繁殖量低于野生菌。     

植物杀虫剂苦皮藤素对柑橘潜叶蛾控制效果的研究

用植物性杀虫剂苦皮藤素Ⅳ(KPT乳油)有效成分30、15mg/kg防治柑橘潜叶蛾,采用离体叶片浸叶法处理5s,3d后,幼虫校正虫口减退率分别为89.63%、63.02%;枝梢浸液法处理5s,5d后,潜道伸长度校正抑制率分别为76.12%和41.51%;10d后,对秋梢的相对保叶效果分别为69.34%和52.29%。结果表明,采用KPT乳油有效成分30mg/kg对柑橘潜叶蛾有较好的控制作用,对柑橘秋梢有良好的保护效果。     

早熟、优质、耐贮运西瓜新品种‘爱耶一号’

 ‘爱耶一号’是采用自交分离、杂种优势利用, 并结合化学诱变而育成的早熟西瓜新品种, 具有成熟早、丰产、抗病、品质佳、果肉红、抗裂果、耐贮运、适应性广等优良特性。     

甜橙新品种‘兴国甜橙3-5’

 ‘兴国甜橙3-5 ’是从实生‘兴国甜橙’后代中选出的新品种。果皮橙黄至橙红色。果实近圆球形, 平均单果质量167. 7 g , 可溶性固形物含量13. 6% ~ 15. 1%, 总糖含量10. 3% ~ 11. 5%, 总酸含量0. 47%~ 0. 93%, 可食率79. 27%~ 80. 75%, 果汁率65. 18%~ 65. 96%, 11 月上旬成熟。     

一个马铃薯Y病毒山东分离物的分离与鉴定

 从具有典型花叶症状的马铃薯叶片中分离到马铃薯Y病毒(Potato virus Y,PVY)(本文称PVY-SD-TA分离物),扩繁后,提纯病毒,电镜下可观察到700~900 nm×11 nm的病毒粒体,病组织超薄切片观察可见风轮状的内含体结构,寄主反应特性研究表明其能侵染2科13种植物。SDS-PAGE电泳检测病毒编码的外壳蛋白亚基的分子量为33 kDa。以PVY-SD-TA基因组RNA为模板,应用RT-PCR方法和特异引物合成了外壳蛋白基因。对cDNA全序列分析表明,PVY-SD-TA CP基因核苷酸序列与N株系的同源性为96%,与GenBank中登录序列号为AJ390306的O株系分离物的同源性最高,为99%;与国内不同学者报道的PVY中国流行株的同源性分别为96%,97%和98%。通过以上生物学特性和分子水平的研究将PVY-SD-TA鉴定为普通株系(PVY^O株系)。