方竹属部分种黄酮类成分比较

通过对广义方竹属13个种叶片的化学分析,在8个种中得到了黄酮类成分,包括木犀草素、异荭草甙以及木犀草素,苜蓿素、香叶木素,柯伊利素、洋芹素的糖甙。实验结果的聚类分析倾向于支持将方竹属、筇竹属合并为一个广义方竹属的观点。     

乳牛口服过量维生素A对维生素D3及钙磷代谢的影响

从北京郊区某农场选取１０头健康成年黑白花乳牛，随机分为试验（５头，每周每头口服维生素Ａ１００万单位）和对照（５头，不作任何处理）两组。８周以后检测发现，试验组血清钙和无机磷水平呈下降趋势；尾椎骨皮质变薄，密度下降，呈现骨质吸收现象；血清碱性磷酸酶显著下降（Ｐ＜０．０５）；其活性代谢产物２５－羟维生素Ｄ３（２５－ＯＨ－Ｄ３）极显著下降（Ｐ＜０．０１）；１，２５－二羟维生素Ｄ３［１，２５－（ＯＨ）２－     

胭脂萝卜新品种胭脂红3号的选育

胭脂红3 号是以不育系09S77A 为母本，以自交系0484B 为父本配制而成的胭脂萝卜一代杂种。播种至收获100～120 d（天），羽状裂叶，叶片绿色，叶柄及叶脉紫红色；肉质根短圆锥形，皮紫红色，肉深红色，横径7 cm 左右，纵径8 cm 左右，单根质量250 g 左右，色素含量17‰左右；每667 m² 产量1 800 kg 左右，适宜重庆地区作色素提取和泡菜加工品种栽培。     

野生草皮的开发利用

通过对海南野生草皮调查，发现其可用于飞机场，靶场，公路旁边和水库堤坝斜皮等的绿化，并提出了有关绿化措施。     

荧光探针对家蚕和柞蚕核酸燐光的影响

荧光探针双乙锭、苯胺基萘-8-磺酸镁盐和溴化乙锭对家蚕和柞蚕DNA和RNA的燐光均有猝灭作用。其中溴化乙锭对燐光的猝灭作用最强,双乙锭次之,ANS(苯胺基萘-8-磺酸镁盐)的猝灭作用最小。     

水蚀地造林树种生态适应性研究

为了给水蚀地造林选择树种提供科学依据，根据北纬２４°３９＇—３０°０８＇东经１０８°４７＇－１１４°１５＇范围内的１３８６个标准地和１２个树种的６８株林木根系资料，采用定性和定量分析相结合的方法，提出了亚热带水蚀地上２０个树种对土层厚度的生态适用极限值；极限为０—５ｃｍ的有Ｐｉｎｕｓｍａｓｓａｎｉａｎａ等１１个树种，６—１０ｃｍ的有Ｄａｌｂｅｒｇｉａｈｕｐｅａｎａ等７个树种，２１—３０ｃｍ的有Ｃｕｎｎｉｎｇｈａｍｉａｌａｎｃｅｏｌａｔａ等２个树种。极限值小的树种根系有较大的根幅和分布深度，并具匍匐于岩石表面浅土层和沿裂隙生长延伸的特性。土层、土层加母质层厚度和坡位对水蚀地生长的影响较大。各生态因子对不同树种的贡献虽有相似性，但仍有差别。生态因子对Ｄ５的影响全部达显著或极显著水平的树种有Ｓａｓｓａｆｒａｓｔｚｕｍｕ等，部分达显著或极显著水平的树种有Ｐｉｓｔａｃｉａｃｈｉｎｅｎｓｉｓ等。     

长白猪血浆酶活性与胴体性状的相关研究

采用通用线性模型分析研究了 4种猪血浆酶活性与 4个胴体性状的相关关系。结果表明 :GPT对皮厚、γ- GT对胴体长达到显著的正效应 (P<0 .0 5 ) ,Amy对眼肌面积达到极显著正效应 (P<0 .0 1)     

Effects of aflatoxin G1 on proliferation and TNF-αsecretion of human peripheral blood mononuclear cells in vitro

AIM: To explore the effects of aflatoxin G₁(AFG₁ )on proliferation and TNF-α secretion of human peripheral blood mononuclear cells(HPBM) in vitro. METHODS: The effects of AFG₁ on proliferation of HPBM were analysed with flow cytometric (FCM) DNA analysis and MTT bioassay, while that on TNF-α secretion was detected with ELISA.RESULTS: FCM analysis revealed that 6 h after treatment, proliferation index(PI) of 1000 μg/L AFG₁ treated HPBM was significantly higher than that of control. 24 h after AFG₁ treatment, stimulating effects on proliferation was found in HPBM treated with AFG₁ at 200 μg/L and 1 000μg/L.Regression analysis showed that PI was postively correlated with the concentrations of AFG₁ in the concentration range from 0 to 1 000μg/L( r=0. 5122 and 0.5119 respectively,P＜0.05).MTT bioassay showed that the A value of the cells treated with AFG₁ at 2 000 μg/L was higher than that of the control. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) results showed that AFG₁ at a dose of 100 μg/L could significantly inhibit lipopolysaccharide-induced TNF-α secretion.CONCLUSION: AFG₁ could stimulate the proliferation of HPBM and could decrease TNF-α secretion at certain concentration.     

莴苣花叶病毒病的研究Ⅰ。病原鉴定

 由山东济南和泰安两市郊区大面积受害的莴苣(Lactuca sativa L.var.asparagina L.)上分离到一株病毒。莴苣上症状为系统花叶,畸型和褐色斑点。人工接种,在昆诺阿藜和苋色藜上产生局部枯斑和系统症状,千日红上呈不规则斑块,不侵染普通烟、心叶烟、曼陀罗、豇豆、菜豆和黄瓜。传毒试验证明经汁液和蚜虫传播,种子带毒。病毒稀释限点5×10^-1~5×10^-2;失活温度55~60℃;体外保毒期(22~23℃)24小时,经有机溶剂澄清,聚乙二醇沉淀和差速离心可部分提纯。病毒粒子线状,长约750毫微米;病组织超薄切片可见风轮状内含体。试管沉淀法初步测定与马铃薯Y病毒血清呈阳性反应。     

Cloning and expression of interferon-alpha/gamma from a domestic porcine breed and its effect on classical swine fever virus

To further evaluate the clinical impact of recombinant PoIFN-alpha/gamma, PoIFN-alpha/gamma genes from a Chinese domestic big-white porcine breed were cloned using PCR, and expressed in a high-level prokaryotic system. The antiviral activities of rPoIFN-alpha/gamma on vesicular stomatitis virus (VSV), porcine reproductive and respiratory syndrome virus (PRRSV), and classical swine fever virus (CSFV) were investigated in different cell lines. The cloned PoIFN-alpha gene encodes a protein of 166 amino acids and has been named PoIFN-alphac. In a comparison of PoIFN-alphac with reported PoIFN-alphaI genes, eight amino acid substitutions at positions 43 (F to L), 78 (N to D), 86 (Y to C), 104 (A to V), 118 (R to L), 128 (T to P), 151 (S to V), and 156 (R to T) were observed, and resulted in no potential N-glycosylation site in the deduced PoIFN-alpha amino acid sequences. In contrast to PoIFN-alphac, one nucleotide substitution was found at position 462 (A to G), hence 0.1% synonymity is specific for the PoIFN-gamma gene. Both PoIFN-alphac and PoIFN-gamma genes were inserted into a prokaryotic vector pQE30, and expressed in E. coli M15 (pREP4) or SC11103 (pREP4) with the N-terminal six consecutive histidine residues, respectively. rPoIFN-alphac and rPoIFN-gamma proteins were detected by SDS-PAGE and Western blotting analysis at 20.7 and 18.0 kDa, respectively. In addition, the rPoIFN-alphac and rPoIFN-gamma protein were purified using Ni-NTA metal-affinity chromatography, and their anti-VSV, anti-PRRSV, and anti-CSFV activities were surveyed in homologous and heterologous cell lines. The results suggested that rPoIFN-alpha and rPoIFN-gamma could inhibit classical swine fever virus and other important viral pathogens in different cell lines.