Sodium dichloroacetate improves radiosensitivity of U251 cells

AIM: To study the change of radiosensitivity of U251 cells after treated with sodium dichloroacetate (DCA) and further to explore the possible mechanism.METHODS: The U251 cells were divided into 4 groups: control group, DCA group, X-ray irradiation without DCA pretreatment (IR) group and X-ray irradiation with DCA pretreatment (DIR) group. MTT assay was applied to determine the cell viability. The intracellular reactive oxygen species (ROS) were detected by DHE fluorescence. The expression level of Bcl-2 was assessed by Western blot. The percentage of apoptosis of cells was determined by flow cytometry. RESULTS: No difference between control group and DCA group in cell viability (P>0.05) was observed. However, the cell viability of both IR group and DIR group was markedly reduced compared with control group (P<0.05). Furthermore, the viability of DIR group was significantly decreased compared with IR group (P<0.05). The percentage of ROS positive cells was obviously increased in DIR group compared with IR group (P<0.05). The expression level of Bcl-2 was sharply decreased in DIR group (P<0.05) and the percentage of apoptosis of cells was significantly elevated (P<0.05) in DIR group compared with IR group.CONCLUSION: The better antitumor effect was obtained by improving the radiosensitivity through pretreating the cells with DCA, and the possible mechanism was down-regulation of the Bcl-2 expression by developing the intracellular ROS.     

Cellular and ultrastructural changes in the seedling roots of upland rice (Oryza sativa) under the stress of two allelochemicals from Ageratina adenophora

Two sesquiterpene‐derivative compounds, 4,7‐dimethyl‐1‐(propan?2‐ylidene)–1,4,4a,8a‐tetrahydronaphthalene‐2,6(1H, 7H)‐dione (DTD) and 6‐hydroxy‐5‐isopropyl‐3,8‐dimethyl?4a,5,6,7,8,8a‐hexahydronaphthalen‐2(1H)–one (HHO), are the major putative allelochemicals of the aqueous leachates of Ageratina adenophora. A laboratory experiment was conducted, using the hydroponic method, to evaluate the cellular and ultrastructural changes in the seedling roots of upland rice under the stress of DTD and HHO. The subsequent changes were observed in the treated upland rice roots in comparison with their controls. The scanning electron microscopy results showed that the DTD‐treated root tip cells turned into an irregular arrangement and shape and that most of them were wizened, with a poor cytoplasm. In the HHO treatment, the root tips had many irregularly shaped cells, with a greater number of sloughing cells, as well as short, wide cells that resulted in spherical and wider, but shorter, roots. At the ultrastructural level, DTD and HHO induced irregularly shaped and lobed nuclei, increased cytoplasmic vacuolation, reduced ribosome density and dictyosomes, and a reduced number of mitochondria in the cells, which indicated limited protein transportation and a reduced capability to export substances for cell development and growth in the upland rice seedling roots. The overall effect of HHO on the upland rice seedlings was more pronounced than that of DTD.     

阿德福韦与七元瓜环超分子相互作用的研究

利用紫外吸收光谱法(UV-Vis)、红外光谱法(IR)及差示扫描量热法(DSC)等考察了七元瓜环(Q[7])与阿德福韦(PMEA)的相互作用.结果表明:Q[7]与PMEA相互作用形成包结比为1∶1的主-客体包合物,包结平衡常数为(2.81±0.04)x104L/mol;在0＜pH＜6范围内均能观察到主客体的相互作用,在...     

柴达木盆地节能日光温室存在问题与发展探讨

介绍了柴达木盆地节能日光温室的发展现状、存在的主要问题,提出了发展的对策及建议。     

节水灌溉工程运行管理技术现状分析与对策

为了彻底解决水资源严重缺乏的问题,为此水利部门以及其他主管部门大量实施了农田节水灌溉工程,在一定程度上有效解决了水资源缺乏的问题.但是由于在实际施工过程中的管理体系不完善以及部分的农田灌溉工程的使用达不到预计效果,大大降低了工程的效益,缩短了使用时间,实施的工程并不能达到设计的标准.现根据我国目前的节水灌溉工程运行管理技术现状,并分析管理技术在实际实施中的主要问题,提出相应的建议和意见.     

Experimental study on landscape of CFRP cutting by abrasive water jet

To investigate the influence of operation parameters of abrasive water jet on surface roughness of carbon fiber reinforced plastic(CFRP),experimental studies were conducted.The three-dimensional landscape of cutting front was reconstructed according to the measured data byμscan laser confocal microscopy.Fourier spectral analysis was also adopted to study surface structure in detail.It is found that the morphology of cutting front is similar to that of other materials.In the smooth cutting zone,the fluctuation of amplitudes of surface profile is gentler,compared with that in the rough cutting zone.The lower part of the rough cutting zone was characterized by the periodical appearance of peaks and valleys.The roughness of surface increases with the increase of depth.While in the smooth cutting zone and part of rough cutting zone,roughness increases with the increase of traverse speed.For the thickness of samples,in the smooth cutting zone,the roughness increases with the increase of depth.The dominant harmonic component in the surface profile is concentrated in a narrow range from 0 to 10 Hz,and the relatively higher density of frequency from 10 to 50 Hz is shown in the rough cutting zone,which is caused by the interaction between perpendicular abrasive water jet and reflect jet.     

汽车车桥模拟加载系统特性分析

建立了摸拟汽车发动机驱动二次元件的转速控制系统和车桥轮边加载二次元件的转矩控制系统的数学模型.在此基础上,对校正前后的转速和转矩控制系统进行了开环频域特性分析.并利用MATLAB软件,对该系统进行了输出响应特性仿真,依此对系统的控制精度和响应特性进行了较为详细的分析.所得有关结论,为汽车车桥二次调节模拟加载系统的设计提供了依据.     

生物学教学怎样提高学生的生物科学素养

生物学教学的基本任务之一就是提高生物科学素养,结合生物学课程的特点,更新教育观念,改进教学方法,优化教学方式,培养学生的生命科学意识、研究方法和科学态度,并逐步形成科学的世界观.     

Turbo码性能分析与仿真

纠错编码技术可有效地提高或改善移动通信的可靠性,在通信系统中得到了广泛地应用.在详细分析Turbo码编译码结构、译码算法的基础上,对Turbo码的性能进行了仿真.结果表明:Log-MAP算法比SOVA算法运算量大、费时更多;相同迭代次数下,Log-MAP算法比SOVA算法误码率性能好;同一算法下,迭代次数越大,误码率性能越好;帧长越大,误码率性能越好.因此,为了保证通信的可靠性和实用性,应对算法、迭代次数、帧长进行权衡.     

地表覆被等环境条件对黔西北土壤有机氮组分的影响

为研究地表覆被对土壤有机氮组分的影响,选择贵州省毕节市七星关区覆被差异明显的旱耕地(YJ)、林灌地(LT)、灌草地(YH)和撂荒地(CJ) 4种样地类型,检测不同覆被等环境条件对土壤全氮和有机氮组分等的影响。结果表明：①双因素方差分析显示,地表覆被对土壤全氮和非酸解氮有显著影响,而土层深度对酸解总氮有影响,但并未达到显著水平。样地土壤剖面全氮的大小排序为YJ (1.421 g·kg-1)>LT (1.274 g·kg-1)>YH (0.972 g·kg-1)>CJ (0.859 g·kg-1)。②各样地土壤全氮含量与非酸解氮含量相关性达显著水平,这与研究区积温不足导致腐殖质化过程缓慢,碳水化合物等原始形态的含氮物质构成非酸解氮的主要成分有关。即使YH剖面全氮分布有异常,但其酸解总氮也随深度加深而下降,这表明其垂直分布具有一定的共性,且受地表覆被等因素影响有限。③酸解各形态氮的大小关系依次为酸解氨基酸态氮(0.322 g·kg-1)>酸解氨态氮(0.214 g·kg-1)>酸解未知态氮 (0.180 g·kg-1)>酸解氨基糖态氮(0.117 g·kg-1)。方差分析显示,土壤酸解氨态氮受地表覆被的影响达到显著水平,而酸解各形态氮受土层深度影响均不显著。④旱耕地酸解氨基糖态氮均值仅为0.026 g·kg-1,这与农药施用对微生物的毒害作用减少了糖态氮的生物补给有关。结果揭示了地表覆被等环境条件对土壤有机氮组分的影响,可为黔西北改善土壤氮素状况提供理论参考。