In vitro viability of cryopreserved equine embryos following different freezing protocols.

The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos.     

春制越年家蚕卵乙胚子冷藏方法研究

研究结果表明，春制越年家蚕卵丝乙胚子冷藏处理后，其实用孵化率〉９２．３％蚁蚕绝食致半数时数≥１１０ｈ，不良卵发生率〈１０％，发病率〈８．６％，茧质优良，产量平稳，在有效冷藏期限内随时出库催青饲育均安全可靠。     

Physiological and morphological changes in bovine mammary glands following intramammary infusion of recombinant interferon-gamma.

Eleven lactating dairy cows were used to evaluate the response of bovine mammary glands to increasing doses of recombinant bovine interferon (rBoIFN)-gamma. Right front and rear quarters were intramammarily infused with eight different doses (10(2) U to 2 x 10(8) U/quarter) of rBoIFN-gamma; each dose was tested in at least two quarters. Left udder halves served as within animal controls in which quarters were injected with a saline placebo or were not infused at all. Milk secretion samples for compositional analysis were collected from each quarter prior to infusion and at 6, 24, 36 and 48 h following infusion. Animals were slaughtered immediately following the 48 h sampling period and mammary tissue was obtained for morphometric analyses. Milk composition was similar between control quarters and those quarters infused with up to 10(5) U of rBo-IFN-gamma during the entire sampling period. Quarters infused with 10(6) U and 10(7) U of rBoIFN-gamma had higher milk somatic cell counts (SCC) following treatment compared with preinfusion values. Changes in the composition of mammary secretion were most dramatic in quarters infused with greater than or equal to 10(8) U of rBoIFN-gamma as indicated by the significant increase in SCC and milk pH with a concomitant decrease in lactose concentration when compared with pre-infusion values or with control quarters. Morphometric analysis of tissue demonstrated an increase in stroma, a decrease in luminal area, and a marked increase in the number of infiltrating leukocytes in those quarters infused with the higher doses of rBoIFN-gamma.(ABSTRACT TRUNCATED AT 250 WORDS)     

优质籼型不育系Q2A的选育

Q2A是重庆市农业科学研究所和重庆市种子公司用金23B×中九B的F2代与58B×Ⅱ-32B的F2代杂交,再用复交F2代与珍汕97A杂交引入野败型胞质育成的一个优质籼型三系不育系,2003年7月通过重庆市科学技术委员会组织的技术鉴定.该不育系异交习性好;可恢性好,配合力强;米质优(12项测试指标中有7项达部颁一级优质米标准,5项达二级标准);苗瘟3级,叶瘟3级,颈瘟7级,对苗瘟和叶瘟表现为中抗,颈瘟感病.其与成恢047配组育成的优质杂交中籼新组合Q优5号米质优,产量高,抗稻瘟病,2003年12月通过重庆市农作物品种审定.     

确定插齿刀廓形的方法及精度分析

论述了由插齿刀前锥面和螺旋面方程确定的母面廓形,提出了保证插齿刀原始廓形角的3个条件,由此推出了计算廓形角的3种方法,得出了廓形角及基圆半径的计算公式,然后对3种方法的误差进行分析,计算表明第3种方法误差较小,是一种较好方法。     

Effects of aminoglycoside antibiotics on renal antioxidants, malondialdehyde levels, and some serum biochemical parameters

Effects of amikacin, gentamicin, kanamycin, and streptomycin on renal tissue superoxide dismutase, glutathione peroxidase, glutathione and malondialdehyde, serum creatinine, potassium, sodium, total protein, glucose, uric acid, and total bilirubin levels were investigated. All aminoglycoside antibiotics decreased renal tissue glutathione levels.