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111.
球根海棠离体培养的形态发生研究   总被引:5,自引:0,他引:5  
 球根海棠外植体可在同一条件下同时诱导不定芽和胚状体, 经间接途径和直接途径的体细胞胚胎发生频率均很高, 体细胞胚为单细胞起源或多细胞起源。以再生嫩叶为继代中间体, 在较高温度(28 ℃) 下诱导, 可建立高效扩繁体系。纵切芽丛继代形成大量愈伤组织, 愈伤组织继代半年后几乎完全丧失分化能力, 发生激素驯化作用, 而且愈伤组织细胞染色体数目已发生广泛变异。  相似文献   
112.
利用紫茎泽兰栽培田头菇的研究   总被引:9,自引:0,他引:9  
利用紫茎泽兰作为原料栽培田头菇 ,同时利用稻草和棉籽壳为原料栽培田头菇作对照。研究结果表明 ,代料熟料栽培田头菇的培养基配方中 ,以紫茎泽兰为主要原料的配方A效果最好 ,生物学效率比对照的稻草培养基平均增产 80 %以上。利用紫茎泽兰鲜草和干草作培养基的单产量差异不明显。  相似文献   
113.
三尖杉枝叶粉末防治花生根结线虫病   总被引:6,自引:0,他引:6  
采用盆栽及大田小区试验,研究了三尖杉Cephalotaxus fortunei枝叶干粉末对花生根结线虫病的防治效果.盆栽试验结果表明,每盆(2kg土)施用三尖杉枝叶干粉末10、15及20 g三种处理,与对照组相比,初侵染相应推迟4、6、10天,并能减少侵染量,降低根结增长率,显著减轻花生根结线虫病病情.大田小区试验结果与盆栽试验结果相似,每小区(5m2)沟施三尖杉枝叶干粉末120、80、50 g,处理后34及53天的2次平均防治效果分别为89%、82%及50%,施药对照10%益舒宝颗粒剂25g处理(相当于45kg/hm2)为74%.综合使用剂量及花生生长情况,建议沟施150kg/hm2三尖杉枝叶干粉为宜.  相似文献   
114.
 ‘桂淮6 号’是从广西山药资源中选育而得。该品种早熟, 高产, 铁、锌含量高, 分别达20.2 mg/ kg 和12.0 mg/ kg , 薯皮鲜红色。  相似文献   
115.
AIM: To investigate the role of potassium channels in the regulation of intracellular free calcium concentration ( [Ca2+]i) of pulmonary artery smooth muscle cells (PASMCs) in rats. METHODS: The fluorescence Ca2+ indicator Fura-2/AM was used to observe [Ca2+]i of rat PASMCs in normal and chronic hypoxic condition. The influences of potassium channels on PASMCs proliferation were assessed by MTT assay. RESULTS: 1. In normoxic condition, [Ca2+]i was (156.91±8.60) nmol/L, and in hypoxic condition, [Ca2+]i was (294.01±16.81) nmol/L. 2. In normoxic condition, the voltage-dependent K+-channel antagonist 4-aminopyridine (4AP), but not the Ca2+-activated K+-channel antagonist tetraethylammonium (TEA) and the ATP-sensitive K+-channel antagonist glibenclamide (Glib) increased [Ca2+]i. 3. In hypoxic condition, 4AP and TEA caused the rise in [Ca2+]i , but Glib had no effect on [Ca2+]i. 4. MTT assay showed that 4AP increased the value of absorbing light degree (A value) in normoxic and hypoxic condition (0.582±0.062,0.873±0.043,respectively, P<0.01), TEA increased A value only in hypoxic condition, and Glib had no effect on the proliferation of PASMCs. CONCLUSIONS: KV plays an important role in the regulation of [Ca2+]i and proliferation of PASMCs. KCa serves as distinct responsive roles in the regulation of proliferation of PASMCs in hypoxic condition. KATP has no effect on [Ca2+]i and proliferation of PASMCs in normoxic and hypoxic conditions.  相似文献   
116.
AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.  相似文献   
117.
118.
AIM: To study effects of urokinase-type plasminogen activator (uPA) signal transduction on expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in giant cell tumor of bone (GCT). METHODS: Expression of uPAR, MMP-2 and TIMP-3 in GCT tissue was detected by immunohistochemistry. Phosphorylation level of mitogen-activated protein kinase (p44) in uPA/uPAR signal pathway in cultured GCT cells was detected by immunoprecipitation. The expression of MMP-2 and TIMP-3 in cultured cells after treatment with uPA-ATF or anti-uPAR antibody was also detected by Western blotting. RESULTS: 1) Urokinase-type plasminogen activator receptor (uPAR) was positive on the cell membrane and in cytoplasm of some mononuclear stromal cells (MSCs) and multinucleated giant cells (MGCs); 2) MMP-2 was positive in the cytoplasm and on the cell membrane of almost all of MSCs and some of MGCs. The polar distribution of MMP-2 in the cytoplasm of MGCs was especially obvious; 3) The expression of TIMP-3 of some MSCs and MGCs in GCT was much lower than MMP-2. The positive signal also showed a prominent polarity; 4) After treatment with uPA-ATF, the phosphorylation level of p44 in GCT cultured cells was much higher than the control. Addition of anti-uPAR antibody in the cells remarkably down-regulated the phosphorylation level of p44 as compared with the control group, suggesting that uPA-ATF participates cell signal transduction and this reaction can be inhibited by anti-uPAR antibody; 5) uPA-ATF cell signal pathway up-regulated expression of MMP-2 and TIMP-3, while anti-uPAR antibody down-regulated the expression of MMP-2 and TIMP-3. CONCLUSION: These results demonstrate for the first time that uPA-ATF directly regulates the expression of MMP-2 and TIMP-3 by signal transduction pathway, and the over-expression of MMP-2 and TIMP-3 may play an important role in local osteolysis of GCT.  相似文献   
119.
120.
AIM: To evaluate the different conditions inducing mouse embryonic stem cells (ESC) in vitro to differentiate into cardiomyocytes. METHODS: BRL conditioned medium was used to promote the growth of ESC and maintain them in an undifferentiated state. During the inducing process, retinoic acid (RA), DMSO, activin-A and TGF-β1 were used as inducing reagents, and made up six kinds of differentiating medium. Then a three-step method inducing ESC cultured in hanging drops, in suspension and in plating was used to induce the differentiation of ESC. RESULTS: ESC were induced in vitro to differentiate into cardiomyocytes. Of all groups, the highest differentiating rate was observed in the group induced by activin-A (20 μg/L) and TGF-β1 (2 μg/L). CONCLUSION: The inducing conditions including activin-A (20 μg/L) and TGF-β1 (2 μg/L) is very valuable in inducing ESC differentiation into cardiomyocytes.  相似文献   
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