浙江不同稻区耳叶水苋对苄嘧磺隆的抗性比较

采用琼脂法测定了长江三角洲地区宁绍平原和杭嘉湖平原5个稻区(宁波、绍兴、杭州、嘉兴和湖州)稻田耳叶水苋Ammannia arenaria不同生物型对苄嘧磺隆的抗性水平。结果表明,所采集的88种耳叶水苋生物型中,有96.6%已对苄嘧磺隆产生了抗性,其中,低抗(3RI≤10)、中抗(10RI≤50)和高抗(RI>50)的生物型分别占总数的22.7%、53.4%和20.5%。宁波NB0143-01和绍兴SX077生物型对苄嘧磺隆的RI值分别高达124.4和120.4。5个稻区中,绍兴地区耳叶水苋的抗性水平最高,平均RI值为53.1;宁波次之,平均RI值为35.1;湖州、杭州和嘉兴地区的抗性水平相对较低,平均RI值分别为24.1、19.9和18.7。表明稻田耳叶水苋对苄嘧磺隆的抗性程度较高,且在宁绍平原和杭嘉湖平原稻区已普遍出现抗性。其中在宁绍平原苄嘧磺隆对耳叶水苋的选择压较大,长期连续用药可能是该地区抗性水平高于其他区域的重要原因。这是有关耳叶水苋对苄嘧磺隆抗性种群分布的首次报道。     

小贯小绿叶蝉与其主要天敌空间关系研究方法的比较

天敌与目标害虫空间关系密切与否,直接关系到天敌对目标害虫的控制利用。为了选择较为理想的天敌与害虫之间空间关系的研究方法,本文用地统计学法、模糊相似优先比法,扩散系数法、空间生态位重叠指数法和聚块样方方差分析法研究‘黄山大叶种’茶园和‘农抗早’茶园天敌与小贯小绿叶蝉Empoasca onukii 空间关系密切程度,以地统计学法的结果为标准,以其前3位天敌粽管巢蛛Clubiona japonicola、斜纹猫蛛Oxyopes sertatus和鳞纹肖蛸Tetragnatha squamata的位次之和为据,根据位次差别评论5种研究方法研究结果的一致性。聚块样方方差分析方法研究结果与地统计学方法的结果差别较小,其次是扩散系数法和模糊相似优先比法,空间生态位重叠指数法研究结果与地统计学方法的结果差别较大。按照天敌与小贯小绿叶蝉空间关系密切程度位次分析,粽管巢蛛、斜纹猫蛛和鳞纹肖蛸是与小贯小绿叶蝉空间关系密切的前3种天敌。本文是对多种研究方法研究结果一致性进行比较的一种尝试,为分析天敌与害虫空间关系选择研究方法提供参考。     

半干旱栽培稻田不同秸秆覆盖材料的产量效应

采用田间观测的方法,分析了不同覆盖材料对半干旱栽培条件下杂交水稻田间茎蘖数、干物质积累和土壤水分的影响,探讨了不同覆盖材料的产量效应。结果表明:(1)在川西北丘陵旱区进行秸秆覆盖有利于增加非灌溉期田间土壤贮水量,提高田间茎蘖数;秸秆腐烂所释放的养分,缓解了稻田磷钾供应相对不足的现状,有效提高了杂交稻的结实率、有效穗数和千粒重;覆盖处理稻田0~0.5 m土层贮水量较不覆盖处理增加2%~13%,田间茎蘖数增加4%~15%,有效穗数增加4%~8%,结实率增加3%~7%,千粒重增加0.6%~1.7%。(2)在灌溉水源没有保证的丘陵旱区,将半干旱栽培与秸秆覆盖相结合,可有效提高杂交水稻产量。冈优725和D优363实收产量平均值在油菜荚壳覆盖、麦草覆盖和麦糠覆盖处理下分别较对照增加10.2%、7.0%和7.7%。     

黄河近80年径流长程相关性诊断分析及趋势预测

为研究黄河径流的长程相关规律,利用非趋势波动分析法对黄河近80年月径流序列进行了分析,初步得出以下结论:黄河近80年月径流存在内在的长程相关性;长程幂律相关的标度指数а≌0.39;尽管大于11.3a尺度区间的а与小于11.3a尺度区间的а有所不同,但可能由于所分析的径流序列长度受到限制,没有足够的理由判断11.3a是个交叉点;通过波动分析进行趋势预测,在未来十几年,黄河径流的变化可能会成下降趋势。     

春季西兰花品种比较试验

对6个西兰花品种进行比较试验,旨在选出适宜宁波市春季西兰花栽培的品种.结果表明,宁波市春季西兰花栽培主栽品种首选优秀,其商品性好、适应性强;可搭配种植春秀70、EQB8等品种,二者花球紧实、蕾粒细腻、品质好,产量高.     

优质抗病厚皮甜瓜新品种苏甜2号的选育

苏甜2号为中早熟厚皮甜瓜杂交种。全生育期95d左右,果实发育期约35d。植株长势较强,抗白粉病和蔓枯病,较耐低温,易坐果。果实短椭圆形,果皮白色、光滑无网纹;肉厚3.8～4.2cm,果肉浅绿色,肉质软嫩,具有浓香味,中心可溶性固形物14.7%～16.8%。耐贮运。单果质量1.0～2.0kg,667m2产量2000～2300kg。适于华东地区及气候相似地区保护地栽培。     

药食兼用特菜-垂盆草

垂盆草(Sedum sarmentosum Bunge)是景天科(Crassulaceae)景天属植物,适应性广,易栽培,富含16种人体所需氨基酸和多种微量元素,锌、硒、铜、锗、锰等5种微量元素含量要高出日常蔬菜、水果类食物3～10倍,具有保肝护肝、抗癌、改善免疫力、治疗高血压和改善妇女更年期生活质量作用,适合城市近郊露天栽培和保护地周年栽培.     

Response of CREG1 promoter to serum starvation in human vascular cells

AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression.     

嫁接对西瓜根际微生物种群数量及群落结构的影响

分别采用微生物培养的方法、16S rDNA PCR-RFLP方法和phl D基因PCR-RFLP方法研究了嫁接对西瓜根际微生物种群数量、细菌群落结构及拮抗菌（2, 4-DAPG产生菌）群落结构的影响。结果表明：① 嫁接西瓜根际细菌和真菌数量有所提高,而放线菌数量则有所减少。② 嫁接西瓜与自根西瓜具有1个相同的主要根际细菌基因型;不同砧木嫁接的西瓜与自根西瓜也分别具有不同基因型的细菌。③ 嫁接西瓜具有与自根西瓜不同的主要根际拮抗菌基因型。     

Hemin induces and sustains the phosphorylation of Erk1/2 in human umbilical vascular endothelial cells

AIM: To investigate the relationship between hemin and Erk1/2 activation in human umbilical vascular endothelial cells (HUVECs). METHODS: Cultured HUVECs were separately incubated with hemin or H₂O₂ for different times. Subsequently Erk1/2 phosphorylation and total Erk1/2 were determined by Western blotting assay. Flow cytometry was employed to determine the cell cycle distribution. RESULTS: Hemin at the concentration of 1-10 μmol/L induced the phosphorylation of Erk1/2 in HUVECs, and sustained the phosphorylation of Erk1/2 for three hours. The duration of phospho-Erk1/2 induced by hemin was much longer than that in H₂O₂ control (3 h vs 30 min). CONCLUSION: Hemin induces and sustains the phosphorylation of Erk1/2 in HUVECs, which indicates that the effect of hemin on the Erk1/2 activation may be one of pharmacological target of hemin.