Mechanism of RhoA on vascular reactivity following hemorrhagic shock in rats

AIM: To observe the mechanisms of RhoA on vascular reactivity following hemorrhagic shock (HS) in rats. METHODS: The superior mesenteric artery (SMA) in rats subjected to hemorrhagic shock was adopted to assay the vascular reactivity via observing the contraction initiated by norepinephrine (NE) with isolated organ perfusion system. Meanwhile, the effects of Rho kinase, myosin light chain phosphatase (MLCP), myosin light chain kinase (MLCK) on RhoA regulating vascular reactivity were observed. The effects of RhoA agonist U-46619 and inhibitor C3 enzyme on the activities of Rho kianse, MLCP, MLCK and phosphorylation of MLC20 in the vascular smooth muscle cells (VSMC) with hypoxia were also measured. RESULTS: As compared to control group, the cumulative dose-response curves of SMA to NE at 2 h after shock shifted to the right, the maximal contractions (Emax) of NE was significantly decreased. RhoA agonist U-46619 increased the vascular reactivity in the late period of shock. C3 enzyme abolished U-46619 induced the increase in the contractile response of SMA to NE. Rho kinase inhibitor Y-27632 decreased U-46619-induced the increase in the vascular reactivity, MLCP inhibitor calyculin further promoted the increase in the vascular reactivity. However, MLCK inhibitor had no effect on the U-46619-induced change of vascular reactivity. After hypoxia, the activities of Rho kinase and MLCK, and the level of MLC20 phosphorylation were decreased, MLCP activity was increased. RhoA agonist U-46619 increased the activity of Rho kinase and phosphorylation of MLC20, decreased the activity of MLCP, but had no effects on MLCK activity. CONCLUSION: RhoA plays an important role in the regulation of vascular reactivity following shock. The mechanism is closely related to regulating the activities of Rho kinase and MLCP, and increasing the phosphorylation of MLC20 in VSMC.     

聚L-乳酸改性麦草共混物材料的研究

以聚L-乳酸(PLLA)和麦草(WS)为原料,以N-甲基吗啉-N-氧化物(NMMO)为溶剂,采用溶液共混法制备了PLLA/WS共混物。首先将麦草溶解在NMMO后,再添加聚L-乳酸并溶解,制备聚L-乳酸与麦草的共混溶液。共混溶液采用浇膜法制备PLLA/WS共混物,采用差示扫描量热法、X射线衍射法、傅里叶变换红外光谱、热重分析和扫描电镜等方法对所得的共混物进行表征。结果表明,聚L-乳酸与麦草可以形成均匀的共混溶液。共混物中PLLA与麦草组分之间具有较强的相互作用,相容性较好。当共混物中PLLA的质量分数为50%时,共混物可以形成结晶结构,且随着PLLA含量的增加,其结晶更加完美,熔点与热稳定性提高。共混物薄膜断面结构较为致密,这说明PLLA与麦草相容性较好,混合均匀。通过调节聚L-乳酸和麦草的配比,可以制备不同性能的生物高分子材料。     

普陀水仙花大球栽培技术试验初报

对普陀水仙花采用优质种球繁育技术、湿生栽培技术、种球阉割技术、病害综合防治技术和氮、磷、钾合理配方施肥技术5项技术措施后,其病害减轻,分生球减少,主球的大球率从原来的30%以下提高到60%以上.     

泰国政府投巨资收储农产品

以三色竹芋的侧芽为外植体,研究了三色竹芋不定芽诱导、增殖和生根的组织培养过程,并研究了6-BA浓度和继代次数对叶片颜色的影响。结果表明：以三色竹芋的侧芽为外植体,经过芽的分化、增殖和生根可获得批量组培苗;降低6-BA的浓度,减少继代次数,有利于保持叶片的颜色。     

果胶酶在制备澄清型香蕉汁中的工艺研究

澄清型香蕉汁的制备中,果胶酶应用的工艺条件对产品的品质有很大影响。分别考察了果胶酶的添加量、温度、反应时间等因素对香蕉汁澄清度的影响,并通过正交试验发现,反应温度55℃,反应时间为150 min,果胶酶质量浓度为0.09 g/L,果汁的透光率最大为76.74%。     

甘肃不同地区油松针叶氨基酸成分分析研究

油松（Pinus tabulaeformis Carr．）是近年来引入干旱地区的主要造林树种,目前甘肃省已建立万亩油松苗育基地,进行大面积示范推广,主要分布于陇东、陇南、天水等地区。油松的饲用价值和药用价值是针叶,针叶富含多种营养成分,可制成松针精油、松针软膏、松针粉、抗癌扶正冲剂、松针饮料等产品,特别是以石油醚脱脂后的松针用乙醇提取混合氨基酸,作为饲料添加剂,可以促进动物生长,增强抗病能力。但其地域不同,营养成分含量也不尽相同。     

宁夏固原市草畜产业发展运行机制探讨

草畜业生产方式的转变,是一项系统工程,具有复杂性和前瞻性,涉及到农、林等相关行业,与环境保护、城镇建设密切相关。本文根据当地草畜业生产实际,提出了固原市草畜业生产发展机制方面的一些创新思路。     

益生元及其应用研究进展

目前,对肠道菌群的调整手段主要有2种：活菌补菌（即益生菌补菌）和自身肠道菌增殖（即益生元补菌）。主要叙述了益生元的概念、种类、生理机能及应用现状和趋势。     

猪生长激素(PST)脂质体代谢动力学的研究

在猪体内注射脂质体 ,研究其在体内的药物动力学 ,分析其血浆中生长激素浓度随时间的变化曲线 ,确定其释药模型为单室模型 ,求出各项动力学参数 ,结果为PST脂质体的缓释效果显著 ,缓释时间长达7天以上     

植物衰老的分子机制及其调控

综述了植物衰老的意义和影响衰老的内部、外部因素 ,并从分子水平上探讨了植物衰老的机制及其遗传学调控