中国巴马小型猪内源性反转录病毒的检测

目的 :对我国特有巴马小型猪内源性反转录病毒 ( porcine endogenous retrovirus,PERV)的存在与 m RNA的表达情况进行检测 ,了解巴马小型猪内源性反转录病毒的携带情况。方法 :根据以往建立的 PCR、RT-PCR检测方法 ,对来自于巴马小型猪外周血淋巴细胞的 DNA和RNA样品进行 PERV核心蛋白基因 ( gag)、多聚酶基因 ( pol)及囊膜基因 ( env)的存在与表达进行检测 ;同时 ,根据目前通用的 env基因分型方法检测 PERV env-A、env-B、env-C的存在与表达。结果 :在 1 2个被检的 DNA样品中均检出了PERV特异性 DNA的存在 ;同样 ,在 1 2个被检的 RNA样品中均有 PERV特异性 RNA的表达 ,且所表达的 PERV均为 A型和 B型 ;其中有9个 DNA样品检测出 PERV-C型的存在 ,所有样品中均未检出 C型 PERV的表达。结论 :检测结果表明 1 2个被检巴马小型猪基因组中存在着内源性反转录病毒序列 ,且能以 m RNA的形式表达 ,这一结果为我国特有小型猪的开发、利用及其病毒安全性评价奠定了基础。     

Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.     

The use of a Giardia vaccine as an immunotherapeutic agent in dogs.

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.     

矸酆恐?,垩白率与糙米率、蛋白

Χ灾绷吹矸酆康挠跋焓钦?,直接影响比间接影响大。Study on the correlation between rice quality and agronomic traits of quality riceNIE Cheng rong, WEN Yu hui, WANG Yun bo, CHEN Ming zhong,LI Mei, LIU Dao quan(Agronomy Department, Foshan University , Nanhai 528231,China)The correlative analy     

Multi-sectoral Participatory Urban Forestry Development in Philippines Metropolitan Manila

The Luntiang Kamaynilaan Project (Metro ManilaGreening Project) launched in 1988 planted trees inmajor thoroughfares, center islands, school premisesand other idle public lands, was basically a governmenteffort. When the funds ran out, the project also fizzledout. In both projects however, it is evident that the keyfactor is peoples participation.Gaining lessons from the past two projects, thegovernment established a collaborative effort ofgovernment and non-government organizations. TheCl…     

多层喷射沉积制备耐热铝合金管坯热流分析(Ⅱ) --沉积过程热流分析

通过建立沉积阶段热流分析模型，对多层喷射沉积制备耐热铝合金管坯过程中喷射流沉积后的凝固和冷却进行了分析．喷射流沉积后与基体／沉积坯的传导热交换占热输出率的90％以上，在沉积过程中，沉积面的温度变化呈周期性的升降．计算结果表明，多层喷射沉积采用移动坩埚式扫描方式，延长了喷射流沉积时间间隔，喷射流沉积后可以获得10^3K／s以上的冷却速度。     

纤维亚麻新品种黑亚15号选育报告

黑亚15号是2004年2月通过黑龙江省农作物品种审定委员会审定通过同意注册的纤维亚麻新品种。原茎、长麻、全麻、种子产量分别为5641．7、897．2、1282．3、619．okg／hm2，分别比对照增产9．3％、20．2％、12．2％、11．9％。长麻率20．O％，比对照高2．8个百分点。纤维强度261．ON。属高纤、优质纤维亚麻新品种。     

The failure of selenium supplementation to prevent copper-induced liver damage in Fischer 344 rats.

This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury.     

区域证券市场发展潜力评价模型的建立及实证分析

建立了评价区域证券市场发展潜力的数学模型－定权聚类评估模型，并对河南各地区证券发展潜力进行了实证研究。     

弓形虫P30基因及其功能

弓形虫 P30基因所编码的蛋白为弓形虫主要表面抗原 SAG1 ,其约占速殖子总蛋白量的 5 %,SAG1对虫体侵入宿主细胞及其毒力具重要性 ,并有高度免疫原性和免疫保护性 ,被用于弓形虫疫苗的研制和弓形虫感染的分子诊断