农村户用沼气服务体系评价建模及应用——基于2010年壳牌中国能源调查河南省偃师市调研项目

作为新能源的沼气具有巨大的应用前景.近年来,在我国农村沼气事业的发展过程中出现了以服务体系不完善为主的诸多问题,如何科学、合理地评价农村沼气服务体系水平,发现原因及时解决,对我国农村能源战略的发展具有重要的现实意义.本文首先根据实地调研情况并使用专家咨询法构建了服务体系评价的指标体系；其次,建立了基于三角白化权函数的灰色评价模型；最后根据调研数据,运用评价模型分析了偃师市农村沼气服务体系的现状.     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

胶莱盆地北部风尘堆积物光释光年代序列研究

山东省胶莱盆地的高岗部位存在大量的风尘堆积物,对其年代的研究一直存在较大的分歧.对胶莱盆地的潍坊前埠下风尘堆积剖面进行了系统的光释光年代测试,并与其以北16.9km的昌邑县徐林庄剖面的年代进行了对比,初步建立了胶莱盆地风尘堆积的光释光年代序列,研究表明该区风尘堆积主要是晚第四纪以来形成的堆积物.     

棚室专用薄皮甜瓜新品种‘永甜4号’的选育

‘永甜4号’薄皮甜瓜是用地方薄皮甜瓜品种自交系‘白B10’为母本、‘郑引55’自交系‘A97-I-14-63’为父本配制的杂交一代新品种。该品种早熟,全生育期93d,果实发育期31d。在棚室种植坐瓜率高,产量稳定,果皮浅黄色,梨形,单果质量450⁴80g,极少裂瓜,畸形瓜少,中心可溶性固形物含量12%480g,极少裂瓜,畸形瓜少,中心可溶性固形物含量12%¹3%。货架期长,耐运输。2006年通过黑龙江农作物品种审定委员会审定。     

广西县级林地变更调查试点技术方法

为探索广西县级林地年度变更调查技术方法,以鹿寨县为例,运用遥感自动检测技术、森林资源管理信息平台等,对地类及管理属性变化图斑进行变更,形成本期林地数据库和林地变化数据库。文章分析了林地变更调查的关键技术方法,指出了存在的问题,并提出相应的建议。     

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.     

榔榆的嫁接与养护技术

介绍了榔榆嫁接时砧木和接穗的选择、嫁接的时间和方法,以及嫁接后的养护要点。     

通用小型汽油机游车故障的一种非常规排除方法

分析了通用小型汽油机游车的原因，提出了一种较为经济的游车故障排除方法。     

铅和（或）镉对体外培养肾细胞的毒性损伤

在培养液中加入不同浓度的Cd（10、20、40μmol／L）、Pb（10、20、40μmol／L）和Pb-Cd联合（分别为5、10、20μmol／L），来探讨铅镉对肾细胞的作用及可能机制。在显微镜下观察细胞形态、测定细胞凋亡率和细胞周期及上清液脂质过氧化指标。结果表明：20μmol／L以上染毒组细胞皱缩、体积变小，表面有细胞碎片，甚至呈泡沫状或树枝状；铅、镉单独和联合染毒组GSH—Px、SOD活性随染毒剂量的增加，呈逐渐下降趋势（P〈0．05）．而MDA含量和凋亡率则呈升高趋势（P〈0．05），并存在剂量和时间效应。镉和铅也能使细胞周期停滞。Pb、Cd联合可加剧细胞损伤。     

叶菜型甘薯大棚越冬栽培薯苗存活率及其产量性状表现

为提高叶菜型甘薯的繁殖系数,保证种植种源的持续供应,采用薯苗大棚栽培越冬留种,研究不同品种和节位薯苗越冬栽培的薯苗地上和地下部性状。结果表明:叶菜型甘薯福菜薯18和鄂菜薯1号的茎尖薯苗和二节薯苗越冬存活率最高为77.50%,最低为66.25%;鄂菜薯1号茎尖薯苗越冬苗株高最高为29.3cm,茎尖薯苗株高均与二节薯苗的株高差异显著;二节薯苗分枝数、薯苗产量最高分别为62.77个/m2、493.34kg/667m2,均显著高于茎尖薯苗;越冬地下茎存活率以二节薯苗优于茎尖薯苗,存活率在73.75%~82.50%;茎尖薯苗的柴根产量和数量均优于二节薯苗,分别达128.89kg/667m2和16.1个/m2。结论:以二节薯苗栽培越冬,其来年薯苗分枝、产量、地下茎性状均优于茎尖薯苗,薯苗直接栽插繁殖、地下茎萌发繁殖速度均快于柴根。