广西矿区大气沉降与稻米重金累积的关系分析(英文)

笔者就大气沉降的概念、来源及其危害作了简要的概述,并对广西矿区大气沉降与稻米重金属累积的关系作简要分析,以期对广西矿区重金属沉降污染实施有效的风险预警,确保稻米安全生产提供参考。     

Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.     

开展农林工程注册监理工程师继续教育培训的若干思考

随着林业建设的不断发展,林业各领域对农林工程注册监理工程师的需求量逐年增加,切实提高农林工程监理队伍的文化素养和业务能力显得尤为必要.针对当前农林工程注册监理工程师继续教育培训中存在的学习态度不端正、工学矛盾突出、培训手段单一、培训内容针对性不强等问题,建议以现代培训理念为指导,加强培训需求调研,因材施教,加强师资库、教材库、案例库“三库”建设,提升教学质量.     

仿生法评定抗草甘膦玉米和转Bt基因玉米的酶水解物能值的研究

本试验旨在分析抗草甘膦玉米和转Bt基因玉米原料及饲粮与同源非转基因玉米原料及饲粮体外总能消化率以及酶水解物能值,为转基因玉米的营养实质等同性仿生评定方法的研究提供参考。试验采用单因素完全随机设计,使用单胃动物仿生消化系统模拟饲料原料和饲粮在鸡胃肠道的消化过程,分析同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应的3种玉米-豆粕饲粮在不同体外模拟消化阶段的干物质消化率、总能消化率和酶水解物能值的差异。结果表明:同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应饲粮在常规概率成分含量上是相似的。抗草甘膦玉米及饲粮与同源非转基因玉米及饲粮相比,在干物质和能量胃消化率、全消化道消化率及酶水解物能值上均没有显著差异(P0.05)。转Bt基因玉米全消化道总能消化率低于同源非转基因玉米(P=0.03,变异系数=0.50%),对应玉米饲粮的酶水解物能值则高于同源非转基因玉米饲粮(P=0.02,变异系数=1.12%),但均处于仿生消化系统测试的误差范围内(变异系数≤1.64%)。由此可见,抗草甘膦玉米的酶水解物能值与同源对照玉米没有差异,而转Bt基因玉米存在统计学意义上的差异,但所有的测值均处于仿生消化系统的测试误差之内。仿生法发现的差异是否具有生物学意义有待体内试验验证。仿生法可为转基因饲料营养等同性研究提供一种新方法。     

Nutrient uptake requirements with increasing grain yield for rice in China

Improved estimates of nutrient requirements for rice（Oryza sativa L.）in China are essential to optimize fertilization regulation for increasing grain yields and reducing the potential of environmental negative influences,especially under high-yielding intensive systems.A database involving rice grain yields,nutrient concentrations and accumulations collected from on-field station experiments in the literatures published from 2000 to 2013 in China was developed to understand the relationships between grain yields and plant nutrient uptakes,and to quantify nutrient requirements for different yield levels.Considering all data sets,rice grain yield ranged from 1.4 to 15.2t ha^-1with the mean value of 7.84t ha^-1,and ca.10.4%of yield observations were higher than the yield barrier level of 10 t ha^-1.N requirement to produce one ton grain was 21.10 kg for the yield range<4.0t ha^-1with a high variation of 45.8%.Except of the yield range<4.0t ha^-1,the values of N requirement,firstly increased from 18.78 kg for yield range 4.0-5.5 t ha^-1to 20.62 kg for yield range 7.0-8.5 t ha^-1,then decreased slightly to 19.67 and19.17 kg for the yield range 8.5-10 and>10 t ha^-1,respectively.Phosphorus（P）and potassium（K）requirements showed increasing trends,from 3.51 and 19.87 kg per t grain for<4.0 t ha^-1yield range to 4.10 and 21.70 kg for>10.0t ha^-1range.In conclusion,nutrient requirement varied with increasement of grain yield,and N,P and K presented various response trends,increasing,declining or stagnating,which would be of great benefit for improving fertilizer strategies.     

涉农专业青年教师以竞赛为平台的专业成长有效途径研究

在信息技术飞速发展的当今社会,对教育工作者提出了越来越高的要求。在专业教师队伍中,青年教师已逐渐成为主体,而想要将这支年轻的队伍打造成一支中坚力量,成为师资队伍建设的重心,也是当今教育资源建设的一项重要任务。本文以涉农专业青年教师为研究的出发点和立足点,通过搭建各种竞赛平台,研究促进青年教师的专业成长的有效途径。     

普通玉米新品种试验初报

引进11个普通玉米进行试验,结果：登海661、郑单136、登海3622、济单7号比CK增产,登海661综合性状表现较好,可扩大示范种植。     

梨属野生种质资源SRAP-PCR反应体系优化研究

采用正交直观分析法和新复极差法对影响梨属野生种质资源SRAP-PCR反应的5种因素(Mg2+浓度、d NTPs浓度、引物浓度、Taq DNA聚合酶、模板DNA)4个水平进行优化筛选。结果表明,优化后的梨属SRAP-PCR反应体系为25μL,包括10×PCR buffer 2.5μL,2.0 mmol/L Mg2+,200μmol/L d NTPs,0.4μmol/L引物,1.5 U Taq酶,60 ng模板DNA。