Different concentrations of intracellular calcium in uterine myometrial cells at term and non-term

AIM:To investigate different intracellular concentration of Ca²⁺ in uterine myometrial cells at term and non-term.METHODS:The living cells suspensions were made to measure intracellular Ca²⁺ concentrations after stainned by calcium fluorescent indicator Fluo-3 AM, then examined by laser scanning confocal microscope (LSCM).RESULTS:Intracelluar Ca²⁺ showed very stronger red positive signal in myometrial cells at term than that in non-term cells. [Ca²⁺]i were (35±8.1) nmol/L at non term and (75±7.3) nmol/L at term, which had significant difference compared with each other (P＜0.05).CONCLUSION:Increase in [Ca²⁺]i in myometrial cells might play a very important role labor.     

鸭肝炎自然病例与鸭肝炎病毒人工发病病例组织病理学观察

到目前为止，对鸭肝炎I型作组织病理学研究的工作可分为下列几种：鸡鸭肝炎I型标准毒株人工发病雏鸭肝、脾、脑等多种器官作组织病理学观察；对鸭肝炎I型野外病例肝、脾、脑等作组织病理学观察；对鸭肝炎I型人工感染鸡胚肝脏等几种脏器作组织病理学观察等。国外Sandhu等还对鸭肝炎I型变 异株感染雏鸭作组织病理学观察。本文报道了对珠江三角洲有变异倾向的鸭肝炎I型自然病例和6株野毒人工发病病例的组织病理学观察结果，观察包括肝、脾、胰、脑、心、肺、肾、、腺胃、小肠、法氏囊等10种脏器，并对各器官的病变进行具体描述与统计。为国内外研究DHVI型变异株感染的组织病理学研究提供了更丰富详实的资料。     

Effect of tissue factor on intravascular migration of tumor cells

AIM: To evaluate the effect of tissue factor on intravascular migration of tumor cells.METHODS: Expression of tissue factor in tumor cells (HT1080) was analyzed by flow cytometry and immunofluorescence staining. An in vitro model was used to observe intravascular migration of tumor cells. RESULTS: High expression of tissue factor was observed in tumor cells (HT1080). The antibody for tissue factor inhibited intravascular migration of tumor cells. CONCLUSIONS: Tissue factor stimulated tumor metastasis through promoting intravascular migration of tumor cells.     

The Optimized Design of The Fuzzy Controller(Ⅱ)——the disquisition of optimized triangle subjection function

1　Introduction　　 The arithmetic of the fuzzy consequence deals with the rules of the fuzzy control,itscomplexity relies on the definition of the subjection function within the words of the fuzzyrules.Choosing some simple subjection function which can reflect the results of fuzzy conse-quence can simplify the calculation of the fuzzy consequence.The subjection function of fuzzyquantity is bell- like(Xiao Wei- qu. 1 992 ;Wu Wan- duo and Wu Wan- zhao,1 988) ,such asFig.1 ;but in the practic…     

发展商品林——新世纪初江西林业建设的重点

根据国家林业局的区域划分和江西实际，提出江西新世纪初期林业建设的重点是发展商品林。通过调查提供了一批商品林建设取得成功的典型，以论证建设商品林的可行性。提出了确定经营类型，深化林业改革，实施林业产业化经营和规范政府行为等意见和建议。     

鸡传染性支气管炎病毒中国地方流行株的分离与鉴定

对海南省和广西省的几个发生呼吸罗音、鸡群全群发病、死亡率不高的疑是鸡传染性支气管炎病（IB）的鸡群，进行了病毒分离和鉴定。结果分离到了4株IBV分离株（HaN-1/95、HaN-2/95、GX－1／98、GX-2/98），并对分离病毒进行了病毒的致病性、病理组织切片、鸡胚矮小化、对NDV的干扰、电镜特征等生物特性鉴定及IBV基因组特异性3′末端非编码区的一段保守序列的RT－PCR和巢氏PCR鉴定。结果表明4株IBV分离株，均能产生呼吸困难、罗音等临床症状；病理组织切片，气管、肾脏等组织表现为上皮细胞受损、固有层充血、出血和淋巴细胞的浸润及组织坏死；对NDV－B1株有明显的干扰作用；其各自的传代物都有明显的致鸡胚矮小化作用；在透射电镜下观察到典型的冠状病毒粒子，多数近似为圆形，直径75-200nm，有囊膜，表面有一圈杆状纤突排列成花冠状，符合IBV的典型特征；基因组3′末端UTR的RT－PCR和巢氏PCR鉴定，4个分离株分别都扩增到293bp和172bp的特异目的片段，通过测序比较毒株间的核苷酸序列同源性在99％以上，与GeneBank中IBV H52等标准株的3′末端UTR的核苷酸序列高度同源。以上结果表明4株分离病毒都是IBV，为下一步的IBV分子流行病学的研究打下了基础。     

割前脱粒中梳脱滚筒和螺旋搅龙位置关系的试验研究

本文对梳脱滚筒和螺旋搅龙的水平及垂直位置进行了研究，分析了不同位置对损失的影响规律，并确定了二者的合理位置关系。     

四川部分种猪场乙型脑炎血清抗体调查

2001年我室从省内已免疫乙脑的8个种猪场采集了597份猪血清进行了乙型脑炎血清抗体检测，结果抗体阳性率仅达32．83％，37．50％的种猪场血清抗体阳性率低于10％，免疫水平低下，不能有效保护种猪免受乙型脑炎感染。应采取积极的预防措施，采用有效的疫苗进行免疫，有效的防止种猪场流行性乙型脑炎的发生。     

Role of ERKⅠ/Ⅱ in the induction and maintenance of the spinal LTP

AIM: The role of external-signal regulated kinaseⅠ/Ⅱ(ERKⅠ/Ⅱ) in the induction and maintenance of spinal long-term potentiation (LTP) is evaluated.METHODS: The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement.RESULTS: (1) PD98059 (100 μmol/L) or SL327 (200 μmol/L), a selective MEK inhibitor, completely blocked LTP induction when applied at 30 min prior to tetanic stimulation.(2) PD98059 or SL327 reversed spinal LTP in a time-dependent manner.At 15 min after LTP induction, PD98059 (100 μmol/L) or SL327 (200 μmol/L) reversed LTP completely, and at 30 min after LTP induction, the inhibitory rate of spinal LTP inhibited by PD98059 or SL327 was 62.5% and 75.0%， respectively.At 1 h after LTP induction, however, the same concentration of PD98059 or SL327 did not affect the spinal LTP.CONCLUSION: Activation of ERKⅠ/Ⅱ in spinal dorsal horn may be crucial for the induction and the early-phase maintenance of LTP of C-fiber evoked field potentials.     

构筑北京新型农业现代服务体系

分析了北京农业现代服务体系建设的现状,在对美国、日本、以色列、韩国的农业社会化服务体系进行归纳和总结的基础上,提出构建北京新型农业现代服务体系的思路和重点。