安氏隐孢子虫ITS-1序列的PCR扩增、克隆及分析

通过对国内三株安氏隐孢子虫(Cryptosporidium andersoni)即GD株、HN株和AH株的rDNA的内转录间隔区Ⅰ(ITS_1)序列进行PCR扩增、克隆、测序和序列分析,旨在确定ITS_1是否可作为C.andersoni分子分类的遗传标记。结果表明:GD株、HN株和AH株的ITS_1序列基本一致,仅AH株有三个碱基的差异;但与GenBank注册的C.muris和C.parvum存在种间差异,而且差异显著。说明ITS_1可作为C.andersoni种的遗传标记,从而为隐孢子虫属的种间鉴定以及进一步的分子流行病学调查和分子诊断学研究奠定了基础。     

施水量变化对毛乌素沙地4种优势植物叶绿素荧光的影响

以毛乌素沙地4种优势植物沙柳、杨柴、油蒿和柠条为对象，人工控制4种施水量来模拟叶绿素荧光对全球降水量变化的响应。结果表明，不同施水量对这4种优质植物的初始荧光、最大荧光、可变荧光和PSII光化学效率均有显著影响，而且4种植物的这些参数对不同施水量的响应差异明显。在157.5mm施水量时沙柳、杨柴、油蒿和柠条以及630.0mm施水量时柠条都受到明显的水分胁迫，表现出明显的光抑制现象。     

Effect of recombinant interleukin-13 on 3T3 fibroblasts in vitro

AIM: To investigate the influence and mechanism of recombinant interleukin-13 (rIL-13) on fibroblasts. METHODS: 3T3 fibroblasts were divided into two groups: the treated group was treated with rIL-13 (80 μg/L, 24 h or 48 h) and the control was without rIL-13 treatment. Transmission electron microscope and Hoechst kit were used to observe morphology of 3T3 fibroblasts in both groups. The activity of proliferation in both groups was investigated and compared by MTT means. Western blot was used to analyze the level of collagen type I induced by rIL-13 in fibroblasts. The levels of IL-6 and IL-8 in the culture supernatants were determined by radioimmunoassay. RESULTS: The more ribosomes and mitochondrions, as well as bigger nuclei were found in the treated group. The production of IL-6 and IL-8, and proliferation ratio of fibroblasts treated with rIL-13 for 24 h or 48 h were increased obviously, compared with the control (P<0.01). The expression of collagen type I protein in treated groups was also significantly higher than that in control (P<0.01). CONCLUSION: rIL-13 upregulates the proliferation of fibroblasts. rIL-13 also has an effect on the production of proinflammatory factors and collagen type I. Taken together, these results suggest that IL-13 may play important roles in the pathogenesis of tissue fibrosis.     

The protective effect of cistanche deserticola Y.C Ma. on thymocytes in septic rats

AIM: To study the protective effect of cistanche deserticola Y. C Ma. on thymocytes in septic rats. METHODS: Cecum ligation perforation (CLP) was used to induce sepsis. Treatment group was treated with cistanche deserticola Y. C Ma. （1.25 g·kg-1·d-1， ig） for 14 days before CLP. Animals were killed 12 h or 24 h after CLP and thmocytes were collected. The ratio of thmocyte apoptosis and mitochondrial membrane potential were determined by the flow cytometry. The ATP activity was detected by spectrophotography. RESULTS: The rate of thmocyte apoptosis significantly increased 12 h after CLP. The ATP activity decreased 24 h after CLP was significant. The extract of desert living cistanche effectively repressed the apoptosis of thymocytes and maintained the mitochondrial membrane potential. CONCLUSIONS: The extract of cistanche deserticola Y. C Ma. protects thymocytes against apoptosis induced by sepsis. Maintaining of mitochondrial membrane potential may be the protective mechanism.     

基于改进YOLOv8模型的轻量化板栗果实识别方法

为实现自然环境下的板栗果实目标快速识别，该研究以湖北省种植板栗为研究对象，提出了一种基于改进YOLOv8模型的栗果识别方法YOLOv8-PBi。首先，将部分卷积（partial convolution，PConv）引入C2f模块中，缩减卷积过程中的浮点数和计算量；其次，引入加权双向特征金字塔网络（bidirectional feature pyramid network，BiFPN），增强多尺度特征融合性能，最后，更改边界框损失函数为动态非单调聚焦机制WIoU（wise intersection over union，WIoU），提高模型收敛速度，进一步提升模型检测性能。试验结果表明，改进YOLOv8-PBi模型准确率、召回率和平均精度分别为89.4%、74.9%、84.2%；相比原始基础网络YOLOv8s，模型权重减小46.22%，准确率、召回率和平均精度分别提升1.3、1.5、1.8个百分点。部署模型至边缘嵌入式设备上，经过TensorRT加速后，检测帧率达到43 帧/s。该方法可为板栗智能化收获过程中的栗果识别提供技术基础。     

南疆绒山羊的培育

南疆白绒山羊是通过对当地山羊的选育和杂交改良，经过近20年的努力育成的。通过本品种选育，横交固定，饲养管理等技术的实施，培育出了体型外貌一致，生产性能高，遗传性能稳定的新品种白绒山羊7．6万余只，并且建成年生产种羊达1000只的种羊生产基地，采取边培育边推广的方法，使阿克苏地区绒山羊平均个体产绒量达175．6g，取得了明显的经济，社会效益。     

抗虫棉群体NPt-Ⅱ标记基因纯度与其抗虫性强度的相关分析

研究了转基因抗虫棉标记基因纯度与生物学抗虫性的相关特性,结果显示:综合平均抗性指数与棉苗群体抗虫标记基因纯度两者呈极显著的正相关,即品种群体抗虫标记基因的纯度愈高,其生物学抗虫性愈强。通过提高转基因抗虫棉Npt Ⅱ标记基因纯度,可显著提高该品种的群体生物学抗虫性;进而通过苗床去杂保纯技术,达到跟踪保持抗虫棉群体抗虫性的目的。     

人类脂肪瘤高速泳动蛋白融合伴侣类基因LHFPL2的组织表达谱及亚细胞定位

应用RT-PCR扩增技术,建立人类脂肪瘤高速泳动蛋白融合伴侣类基因LHFPL2在人体内(肺、胎盘、前列腺、卵巢、肾、肝、心、膀胱、骨骼肌、脑、结肠、睾丸、子宫、胃)的组织表达谱,组织表达谱显示LHFPL2在前列腺、膀胱组织中均有表达。为进一步研究其生物学功能,构建pEGFP-C1/LHFPL2融合载体转染细胞,亚细胞定位显示,LHFPL2在整个细胞中均有分布,以细胞核中较多。LHFPL2基因组织表达谱的建立及亚细胞定位,为进一步深入研究LHFPL2的生物学功能奠定基础。     

农业技术信息传播的现状与未来

目前,农业信息的传播采用的是以政府为主导,以广播电视、报刊杂志为主体的方式。应坚持政府引导,充分发挥大众传播作用,努力提高农民的科学文化素质推动农业技术信息传播。