Estradiol stimulated proliferation and differentiation of prostatic stromal cells through regulation of BPH-1 paracrine

AIM: To characterize the effect of estradiol on proliferation, differentiation and extracellular matrix (ECM) accumulation in stromal cells through regulation of BPH-1 paracrine. METHODS: BPH-1 cells were stimulated with different concentrations of estradiol. Conditioned media (CM) were harvested and their effects on stromal cell cultures were tested. Cell proliferation was determined by MTT assay. mRNA of smoothelin, fibronectin, collagen Ⅳ and transforming growth factor β₁(TGF-β₁) were analyzed by real-time RT-PCR. Western blotting was used to determine smooth muscle myosin heavy chain (SMMHC). ELISA and radioimmunoassay were respectively used to measure fibronectin, TGF-β₁ and collagen Ⅳ protein expressions.RESULTS: Estrodiol stimulated the expression and secretion of TGF-β₁ in BPH-1 cells. The proliferation of stromal cells increased when they were cultured with CM harvested from estrogen treated BPH-1 cells. The mRNA levels of collagen Ⅳ and smoothelin increased in stromal cells treated with CM from BPH-1 cells. The results of radioimmunoassay also showed that the collagen Ⅳ protein level up-regulated in the supernatants and cell extracts of CM-treated stromal cells. A neutralizing antibody to TGF-β₁ inhibited the stimulation of collagen Ⅳ and SMMHC by BPH-1 CM. The expression of fibronectin was only marginally changed in stromal cells cultured in the presence of BPH-1 CM. CONCLUSION: The BPH-1 cells increase ECM accumulation and differentiation of stromal cells through TGF-β₁. Estradiol stimulate differentiation of stromal cells by induction of TGF-β₁ expression. Estradiol stimulate proliferation by influencing the factors secreted from prostatic epithelial cells.     

Effect of different PRA serum levels from patients with β-thalassemia on the proliferation and differentiation potential of the hematopoietic stem cells /progenitor cells of cord blood

AIM: To probe the effect of different panel reactive antibody(PRA) serum levels from patients with β-thalassemia on the proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood.METHODS: 1×105 mononuclear cells (MNCs) isolated from umbilical cord blood were incubated with different PRA serum levels (0 μL,50 μL,100 μL) respectively and complement,inoculated into the methylcellulose cultural system.The proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood by the colony formation assay were detected on day 7 and day 14,respectively.RESULTS: After culture of 7 days,the total colonies and CFU-GM were 88.20±9.41,79.00±11.39 in group A and 88.60±9.12,79.20±10.44 in group B,which were significantly higher than those of 20.60±7.39,15.20±4.66 in group C and those of 4.00±2.05,1.40±0.51 in group D (P<0.01).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).After culture for 14 days,the total colonies and CFU-GM were 216.00±31.10,117.40±24.80 in group A and 213.20±31.06,116.00±19.75 in group B,which were significantly higher than those of 97.80±14.43,32.80±8.10 in group C and those of 31.40±13.41,8.40±4.30 in group D (P<0.01).The CFU-GEMMs were 45.60±8.51 in group A and 42.60±7.03 in group B,which were significantly higher than those of 20.80±6.96 in group C and those of 7.80±6.06 in group D (P<0.05).The BFU-MK was 12.80±4.42 in group A and 11.00±2.74 in group B respectively,which were significantly higher than that of 1.00±0.55 in group D (P<0.05).The CFU-E in group B 17.20±4.03 was significantly higher than that of 5.60±2.87 in group D (P<0.05).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).By the Kendall test,there were negative correlations between the level of PRA serum and the total colonies,CFU-GM on day 7,the total colonies,CFU-GM,CFU-GEMM,BFU-E,BFU-MK on day 14 (tau-b=-0.793,-0.849,-0.808,-0.804,-0.645,-0.674,-0.624,P<0.01).There was a negative correlation between the level of PRA serum and CFU-MK on day 14 (tau-b=-0.466，P<0.05).CONCLUSION: PRA sera inhibit the colony in the colony cultures of the hematopoietic stem cells/progenitor cells in cord blood.The inhibition depends on the level of PRA sera.The higher the level of PRA sera,the stronger the inhibition is observed in our study.     

哈密地区绿洲植被遥感监测及其变化原因分析

植被作为生态环境的重要组成部分,是人类生存环境变化的一个敏感指示变量.选取2002-2005年连续4年7月份的MODIS 250 m×250 m的遥感影像,在EOS/MODIS资料接收处理系统、卫星遥感应用系统平台以及 MAPGIS6.5的支持下,通过预处理、几何校正、归一化植被指数(NDVI)提取以及最大值合成,对东疆哈密地区的植被盖度进行了计算,并按 NDVI 值的大小将地表植被分为3类,计算出了不同植被覆盖的面积,比较了逐年不同盖度植被的面积变化,结合哈密地区的资源分布图进行比较,研究表明:哈密地区2002-2005年低覆盖的植被盖度减少较快,且该区域大多为牧草地,这也说明在那些原本就是低植被覆盖的牧草地区域,脆弱的生态环境正遭受到不断的破坏,正在走向沙化、荒漠化,这主要是由于降水量、人为因素以及载畜量所造成的.     

车辆行驶速度对机动车污染排放影响的实证研究

机动车污染排放主要是由车辆在道路中的行驶造成的,机动车在拥堵情况下所造成的污染排放会明显高于正常行驶状况。文中运用社会学调查抽样的方法,通过搜集大量的出租车票据对北京市目前道路车辆的行驶速度进行了调查,结果表明北京市道路车辆行驶速度普遍偏低。同时,通过对北京市5个交通污染监控点的污染物排放实时数据的分析,得出行驶速度和交通污染排放的相关关系,运用MOVEs模型核算了提高车辆行驶速度所带来的减排效果,可以借鉴国际发达国家和城市的经验,通过采取缓解交通拥堵的方式来有效控制路上移动源的排放。     

杂交马褂木幼林施肥试验

以杂交马褂木幼林为供试对象，进行不同肥料种类和施肥量试验。结果表明：追肥1a后，各施肥处理间树高生长量有极显著差异，以施洞氮复合肥0.25kg／株和马褂木无机专用肥0.25k∥株的树高生长量最大；追肥3a后，各施肥处理间树高生长量有极显著差异，胸径生长量没有显著差异，以施尿素0.25kg／株的树高生长量最大，胸径和树高生长量分别为3.09em和1.85m，比对照分别提高72％和111％。综合成本效益考虑，适合推广的肥料种类和施肥量依次为马褂木无机专用肥0.1ks／株、马褂木无机专用肥0.25k∥株和尿素0.25kg／株。     

青海省北川河流域拟鲶高原鳅的生物学特性研究

以2011年8月至2012年9月间采集的113尾拟鲶高原鳅为试材,运用生物学测定和渔获物统计法对青海省北川河流域拟鲶高原鳅进行了生物学研究.结果显示:青海省北川河流域拟鲶高原鳅种群资源衰竭趋势加剧,形态学特征在不同河段有所变化;体长与全长、体长与肠长的相关性较为合理;体质量与体长的相关性关系式W=0.284 5 L1.3556(r2=0.871 5),体质量与肠长的相关性关系式W=0.128 4 LT1.660(r2=0.820 6),体质量与体高的相关性关系式W=2.243 0 H0.680(r2=0.841 8);摄食率较大,充塞度级别较高,大部分为3级以上,丰满度系数为2.428.试验表明,青海大通北川河流域支流半脑山地区的溪流中发现的拟鲶高原鳅,雌雄比例不合理,性成熟个体较少,个体生长比不合理,亟待保护和增殖.     

试析体育流行和参与体育的关系——一个经验研究的视野

当今社会，流行（时尚）这一社会现象普遍存在于人们社会生活的各个领域，追求流行（时尚）已成为现代人特别是中产阶层女性的一个重要特征。以广州市企业女性白领阶层为研究对象，以文献、问卷、主成分分析和结构方程路径模型分析为主要研究方法，从流行和体育流行的概念入手，通过经验研究描述分析了被调查者接受体育流行的一般概况、接受体育流行的类型和途径、接受体育流行和参与体育的关系、影响被调查者参与体育的因素等，最后提出了本文的研究结论与思考。     

不同颜色防虫网覆盖栽培对豇豆生长发育的影响

研究了不同颜色（白色、黑色、红色、蓝色、绿色、银灰色）防虫网覆盖对栽培豇豆生长发育的影响。从豇豆的生育期、株高、茎粗、叶面积及叶绿素含量指数、产量和病虫害等方面综合考虑,在三亚地区绿色防虫网是较适合豇豆栽培的覆盖材料。     

花生种子大小对产量及品质的影响

在恒温箱和田间条件下,研究2个花生品种不同种子大小对发芽、产量以及品质的影响。结果表明,大粒种子具有较高的活力指数和出苗率,但其发芽率和发芽指数略低于小粒种子和中粒种子。荚果产量以大粒种子最高,其次为中粒种子,最低的为小粒种子。大粒种子较高的荚果产量是与其花量较多,前期有效花率高,根系活力较强以及较多的干物质积累等特性密切相关,这些特性为大粒种子较多的荚果数和高的生产力的形成奠定了良好的基础。花生蛋白质和脂肪含量主要受品种遗传特性和环境条件的作用而表现一定的差异,种子大小对两者影响较小。