SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库

为了分离和克隆辣椒中疫霉菌诱导基因,以接种辣椒疫霉菌的叶片为材料,利用SMART技术构建辣椒疫霉菌与辣椒互作的双杂交c DNA文库。结果表明:该文库容量为3.6×106cfu,重组率88%左右,插入片段集中在300~2000bp之间,平均长度约为800bp,表明获得的文库质量较高,该文库将为分子育种提供重要的基因资源。     

一种新颖的阳离子非病毒基因载体 Chitosan-g-PEI-g-PEG-OH 的性能研究

研究了新型阳离子聚合物Chitosan-g-PEI-g-PEG-OH的性能，重点考察其粒度，基因转染效率与细胞毒性，探讨了其作为基因载体的可能性.通过动态光散射仪（DSL）、透射电镜（TEM）观察了Chitosan-g-PEI-g-PEG-OH与DNA自组装形成的颗粒形态及粒径，Chitosan-g-PEI-g-PEG-OH可复合DNA形成粒径160～210 nm的纳米复合物，适合进入细胞.使用MTT比色法分析Chitosan-g-PEI-g-PEG-OH的毒性并与PEI，PEI-g-PEG-OH比较.选用增强型绿色荧光蛋白(EGFP) 转染Hela细胞，应用流式细胞术检测转染效率.新型阳离子多聚物Chitosan-g-PEI-g-PEG-OH在提高基因转染效率的同时降低了其细胞毒性，有望成为基因转移的有效载体.     

Biodegradation of Linear Alkyl Benzene Sulphonate

The paper researches on the degradation of LAS (Linear Alkyl benzene Sulphonate) by combining AS1.860 immobilized with low intensity ultrasonic irradiation. The paper also discusses the influence of pH, rotary velocity, LAS concentration and the condition of low intensity ultrasonic irradiation on the degradation of LAS, the degradation rate of LAS is the main index of our experiment, the results of orthogonal test shows that low ultrasonic irradiation can increase the metabolizing of microorganism cells and facilitate the biodegradation of immobilized cells to LAS, here we research the degradation condition of 50mg/L LAS simulation wastewater with low ultrasonic irradiation, and the results show that the influence is obvious, the optimization degradation rate is about 83%, nine point five percent higher than that of the immobilized cells without ultrasonic irradiation.     

The Bankruptcy System and the Auction Model of Management Buyout

This paper studied how the auction processis influenced by bankruptcy probability, bankruptcy cost, and the corporatetax rate. The study found thatthe bidding price of the management is negatively associated with bankruptcy cost and probability, and positivelyassociated with the corporate tax. However, the expected profit of the outside investor is positivelyassociated with bankruptcy cost and probability, and negatively associated with the corporate tax. When the bankruptcy cost and probabilityis high enough, the acquisition market would be totally inefficient.     

Deciding Method of Capability Vs. Price of Real Estate

The authors determine the index system and the index weight of capability of real estate by applying Delphi method and AHP method, and found the fuzzy comprehensive evaluation model of capability of real estate. They creates the model of capability Vs. price based on value engineering theory. The authors analysis detailedly deciding method of capability Vs. price of real estate combined with a practice example and application. The method adapts to a house as well as the type of the house. The method resolves the problem which capability Vs. price of real estate was only described by qualitatively for ages, which supplies theory basis to developer and consumer.     

Real Estate Brand Evaluation

At present, in the evaluation process of the real estate value the material form is mainly concentrated on, while the value brought by the real estate brand are usually neglected. In this paper, considering the particularity of the real estate brand evaluation, the method of network analysis is adopted. In the evaluation process, the evaluation method of excess income law are developed by leading the thoughts and methods of the fuzzy set theory, and a systematic assess model is found. Then, an example is given as illustration. At last, the appraise about the application of the model is made.     

Soft-switching Analysis Phase-Shifted-FB-ZVS-PWM DC-DC Converter With Saturable Inductor

Compared with the traditional full-bridge(FB)zero-voltage-switched(ZVS) pulsewidth-modulated(PWM) converter, the FB-ZVS-PWM converter with a saturable inductor has a wider load range with ZVS and smaller circulating energy as well as smaller duty cycle loss. By analyzing the operation principle of the converter, this paper studies design considerations of the parameter bringing about ZVS and dead time of the switch, and gives out the design method, finally simulation testing has been carried out.     

丰两优1号的种植表现及其高产栽培

本文报道了两系杂交稻丰两优1号的特征特性和在温州的种植表现,并提出了与该组合相适应的适时播种、合理密植、水调群体、精量施肥、科学防治病虫害为主要内容的高产配套技术。     

水稻覆膜栽培技术研究进展

水稻是我国主要粮食作物之一,随着社会经济的发展,绿色、优质、高效、生态和安全成为水稻栽培的重要目标。水稻覆膜能有效控制杂草生长,减少除草剂施用,提高肥料利用率,是一项具有增产增效、资源高效利用、绿色生态环保效果的优良技术。综述了覆膜对稻田环境和水稻生产的影响,探讨了水稻覆膜技术在发展过程中覆膜方式、膜的材质以及需求等方面的变化,进行了水稻覆膜机插的效益比较,展望了水稻覆膜栽培技术的发展方向,以期为水稻绿色高效栽培技术的发展提供新方法。     

一种利用气相色谱快速检测瘤胃体外发酵液中三甲胺浓度的方法

本试验旨在建立一种利用气相色谱快速检测瘤胃体外发酵液中三甲胺浓度的方法.瘤胃体外发酵试验设置2个组,对照组(n=4)和试验组(n=4),试验组每个发酵瓶中添加0.0414 g三甲胺盐酸盐,发酵24 h.采用10 mol/L氢氧化钾溶液对发酵液样品进行预处理,使用WEL-PEG20M色谱柱和氢火焰离子检测器对三甲胺进行分...