The kinetics-based enzyme-linked immunosorbent assay for coronavirus antibodies in cats: calibration to the indirect immunofluorescence assay and computerized standardization of results through normalization to control values.

The computer-assisted, kinetics-based enzyme-linked immunosorbent assay for coronavirus antibodies in cats was calibrated to the conventional indirect immunofluorescence assay by linear regression analysis and computerized interpolation (generation of "immunofluorescence assay-equivalent" titers). Procedures were developed for normalization and standardization of kinetics-based enzyme-linked immunosorbent assay results through incorporation of five different control sera of predetermined ("expected") titer in daily runs. When used with such sera and with computer assistance, the kinetics-based enzyme-linked immunosorbent assay minimized both within-run and between-run variability while allowing also for efficient data reduction and statistical analysis and reporting of results.     

小麦品种慢叶锈抗病性的研究

1982—1984年在温室和田间对3个慢叶锈品种:中引305、中引515、中引709,2个快叶锈品种:无沙八号、纳罗的研究表明,小麦慢叶锈抗病品种具有8个抗病特性:(1)田间发病迟、普遍率增长缓慢,严重率低且发展速度慢;(2)潜伏期较长;(3)侵染频率较低;(4)夏孢子堆较小;(5)菌丝和夏孢子堆面积扩展速度慢;(6)产孢能力较弱;(7)对叶锈菌生理小种特异性不明显;(8)对产量影响不显著。慢叶锈抗病性随植株生长而增强,应用病害发展曲线下面积(AUDPC)的A值比侵染速率r值和冈珀茨方程的k值能更好地表达小麦品种的慢叶锈抗病性。利用温室测定苗期潜伏期、侵染频率和田间发病盛期旗叶的严重率指数可以作为小麦慢叶锈抗病品种的选育指标。     

Evaluation of a guarded bronchoscopic method for microbial sampling of the lower airways in foals.

A novel method to reduce contamination of the bronchoscope during microbial sampling of the lower airways of foals was evaluated. Methylene blue (MB) was used as a nasopharyngeal dye marker to assess the relative contamination from the upper airways of bronchoalveolar lavage (BAL) specimens obtained by standard bronchoscopy (SB) and a "guarded" bronchoscopic method (GB). For GB, a clear sterile cellulose sheath was fitted over the bronchoscope in an effort to protect the endoscope tip and channel from contamination. Methylene blue was detected visually in seven of eight BAL samples from foals following SB, but in none of the samples recovered by GB (p less than 0.001). Significantly less MB was detected in BAL by spectrophotometry in the GB group as well (p less than 0.02). The GB was next employed to study the microbial flora in the lower airways of healthy weaned foals (n = 30). Bacteria were isolated from 29 of 30 (97%) BAL samples, and in moderate or large numbers from 26 of 30 (87%) of the foals. Potential pathogens, including Bordetella bronchiseptica, Streptococcus zooepidemicus, Staphylococcus aureus, Mycoplasma felis and Streptococcus pneumoniae, were cultured from the lower airways of foals. In conclusion, the bronchoscope and bronchoalveolar lavage specimens were readily contaminated by a dye marker placed in the nasopharynx of foals, and the degree of contamination was significantly reduced by sheathing the endoscope. This contamination during bronchoscopy may obscure the interpretation of isolates from BAL specimens from foals, which may possess a bacterial flora in the lower airways without cytological evidence of inflammation.     

Comments on the method to test side effects of pesticides on the predatory mitePhytoseiulus persimilis Ath.-Hen

A paper published byKniehase & Zoebelein (1990) describes a new laboratory method to test the side effects of pesticides on the predatory mitePhytoseiulus persimilis and criticizes the laboratory test developed by the International Working Group pesticides and beneficial organisms of the International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS). The present publication discusses the advantages and disadvantages of both methods, responds to the criticism and mention the overall concept of the IOBC/WPRS Working Group including semifield and field test methods.In einer Arbeit vonKniehase undZoebelein (1990) wird ein neues Laborverfahren zur Prüfung der Nebenwirkung von Pflanzenschutzmitteln auf die RaubmilbePhytoseiulus persimilis beschrieben. Die genannten Autoren äußern sich kritisch über das seither von der Arbeitsgruppe Pflanzenschutzmittel und Nutzorganismen der International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS) praktizierte Verfahren. In der vorliegenden Arbeit werden die Vor- und Nachteile der beiden Prüfmethoden diskutiert. Einer Reaktion auf die Kritik folgt eine kurze Beschreibung der gesamten Konzeption der genannten Arbeitsgruppe, die auf einer Kombination aus Labor-, Halbfreiland- und Feldprüfverfahren beruht.     

Somatic cell counts: associated factors and relationship to production.

Factors affecting somatic cell counts and the association between somatic cell counts and milk production were evaluated. Data were collected from 748 Ontario Dairy Herd Improvement Corporation supervised herds that were on production and somatic cell count programs between April 1981 and March 1983. Two data files were created; one, the lactation summary file, contained one record per cow on each of 9406 Holsteins and the other, the test day file, included results of all tests during the complete lactation on each of the above cows. The latter file contained 85,236 records. Multiple curvilinear least squares regression was used to create five separate models. The dependent variables used in the models were natural logarithms (Loge) of the geometric mean of the somatic cell count for the lactation, 305 day milk production and breed class average for milk from the lactation summary file, and loge of the 24 hour somatic cell count and 24 hour milk production from the test day file. The somatic cell count at both the lactation and test day level increased with age up to approximately ten years and thereafter slowly decreased. The variable "days in milk" was not significantly associated with the lactation average somatic cell count. A curvilinear relationship was found between days in lactation at the time of test and the somatic cell count of 24 hour milk production. The somatic cell count increased until approximately 250 days in lactation and thereafter slowly decreased. It was found that the highest cell counts occurred in summer and the lowest in winter.(ABSTRACT TRUNCATED AT 250 WORDS)     

A path model of factors influencing morbidity and mortality in Ontario feedlot calves.

The principles of path analysis and causal modelling are discussed. Path analysis was applied to three data sets to assess the relationship between group characteristics (number per group and "mixing" subgroups of cattle, feeding-management of the group and processing factors (vaccination and prophylactic antimicrobials) and subsequent morbidity and mortality in feedlot cattle. The major findings agree with previously reported results but the timing and pathways of the effects are elaborated. In general, morbidity in week 1 was correlated with morbidity in week 2, which was correlated with morbidity in weeks 3-5. The same was generally true for mortality. In general, morbidity was not strongly correlated with mortality. Lots (unmixed groups) did not arrive in better condition, but experienced fewer subsequent health problems than mixed groups. (Silage-fed lots appeared to do poorly, however this was apparently due to the positive association between lots and vaccination, the latter being detrimental to mortality rates.) The more cattle per group, the greater the health problems in weeks 3-5 postarrival. Prophylactic antimicrobials in the water supply on arrival lead to increased health problems in the three to five week postarrival period. Antibiotic containing starter rations had a beneficial effect on health status in this period. This effect appeared to be partly due to delaying making silage the major ration component in silage-fed cattle receiving antimicrobial containing starter rations. Vaccination against respiratory disease in either of the first two weeks postarrival had detrimental direct and indirect effects on subsequent health status. Vaccination during weeks 3-5 postarrival was not significantly related to health status in that period.     

Genomic variations and antigenic relationships among cytopathic rotavirus strains isolated in Quebec dairy herds.

Twelve isolates of bovine rotavirus, originating from eight dairy herds in Quebec known to have frequent epizootics of diarrhea in young calves in the last five years, were successfully propagated in cell cultures. The 12 isolates produced clear-cut plaques in BSC-1 cells and, except for one isolate, agglutinated human group "O" erythrocytes to an higher titer than bovine erythrocytes. Antisera to each isolate were produced in rabbits and used to study their antigenic relationships. All the isolates shared the group-specific immunofluorescent antigen and were antigenically related as demonstrated by the seroneutralization and hemagglutination-inhibition tests. However, the relationships to the Nebraska rotavirus was quite weak in cases of two Quebec isolates. When the genomes of the various isolates were compared by polyacrylamide gel electrophoresis, at least three different reproducible fractionation patterns could be identified.     

Microorganisms associated with pneumonia in slaughter weight swine.

The lungs of 334 pigs were obtained from two slaughter plants in Minnesota and examined in detail. Macroscopic and microscopic evaluation, direct fluorescence for Mycoplasma hyopneumoniae and bacterial culture were done on all of them and a subsample of 50 were selected for virus culture. Mycoplasma hyopneumoniae, Pasteurella multocida and Haemophilus spp. were detected in 24.0%, 34.1% and 27.0% of the lungs, commonly in conjunction with each other. One isolate of Haemophilus pleuropneumoniae serotype 2 was detected and this represents the first report of its presence in the United States. No virus was detected in any of the lungs. Lungs with both M. hyopneumoniae and Pasteurella multocida had the greatest amount of macroscopic pneumonia (9.8% of the lung). Lungs with M. hyopneumoniae or P. multocida alone had 4.9% and 5.2% of the lung involved with pneumonia respectively. Lungs with Haemophilus sp. Taxon "minor group" had 3.8% of the lung involved which was not significantly different from lungs with none of these organisms being detected (1.6%). There was a positive correlation between the extent of M. hyopneumoniae infection, as scored by FAT and the amount of macroscopic pneumonia present (r = 0.46; P less than 0.001). Likewise, there was a positive correlation between the estimated concentration of P. multocida present, as scored by the relative number of colonies on blood agar and the amount of macroscopic pneumonia present (r = 0.60; P less than 0.001). Microscopically, the amount of lymphoreticular proliferation, polymorphonuclear cells and alveolar macrophages were evaluated.(ABSTRACT TRUNCATED AT 250 WORDS)