南宁市黄曲条跳甲发生为害情况及防治对策

介绍了黄曲条甲的形态特征及其十字花科蔬菜的危害特征,提出了相应的几种防治措施与对策。     

苦瓜专用砧木丝瓜新品种蓉砧1号的选育

＂蓉砧1号＂是一个苦瓜嫁接专用砧木新品种,母本S1-7-2是从中蔬所引进的丝瓜材料S1-7经过2a单株定向选育而成的稳定自交系,父本S1-23-3是从四川省内收集的丝瓜资源经过3代连续自交选育的稳定自交系。该品种根系发达,与苦瓜嫁接栽培时,抗枯萎病能力强,嫁接亲和力高,共生亲和性好,耐涝、耐旱能力强,生长快,长势强,能改善接穗品质,增产达16%。     

羊口疮病毒PCR检测方法的建立与应用

根据羊口疮病毒(ORFV)H2L基因序列,设计合成1对引物,通过确定最佳的PCR反应条件,建立了检测羊口疮病毒DNA的PCR检测方法.结果显示,用这1对引物均能扩增出羊口疮病毒507 bp的特异性片段,同时检测口蹄疫病毒和山羊痘病毒,结果均为阴性,最小检出量为5 pg.该方法具有良好的特异性和敏感性,可对临床组织病料中的ORFV进行快速检测.     

蓟马危害苜蓿的产量损失及防治指标研究

采用人工接虫的方法,研究了蓟马的虫口密度与苜蓿产量损失之间的关系和防治指标。结果表明,苜蓿干草产量损失率(Y)与20枝条虫量(X)的关系为Y=0.1021X-2.2461,相关系数r为0.9874,两者相关性极显著。按照防治的直接收益与成本,确定了宁夏旱地苜蓿和水地苜蓿蓟马的防治指标,即旱地苜蓿蓟马第1茬的防治指标为560头/100枝条,水地苜蓿蓟马第2茬的防治指标为390头/100枝条,第3茬的防治指标为580头/100枝条。     

Study on the role of oxidative stress in the kidneys of diabetic rats

AIM: To investigate the changes of oxidative stress in the kidneys and their roles in nephropathy in diabetic rats. METHODS: Diabetic rats were induced by streptozotocin (STZ). 36 rats were divided into three groups randomly: (1) NC group, normal control rats; (2) DM group, diabetic rats received protamine zinc insulin (PZI) 2U-4U/2 d; (3) DT group, diabetic rats received PZI 9-12 U/kg body weigh/day. 12 weeks later, rats were killed, blood glucose, blood cholesterol, serum creatinine, blood urea nitrogen, HbA1c, urinary creatinine, and urinary protein for 24 h were measured. The activities of antioxidant enzymes in renal cortex, including total superoxide dismutase (TSOD), Cu-Zn superoxide dismutase (Cu-Zn SOD), Mn superoxide dismutase (Mn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and maleic dialdehyde (MDA) were measured by chromatometry. RT-PCR was performed to detect the expression of different antioxidant enzymes mRNA. RESULTS: For all the targets we measured, there was no significant difference between NC and DT groups. Compared with the other two groups, the levels of blood glucose, cholesterol, trigalloyl glycerol, HbA1c in DM group increased significantly. The activities of TSOD, Cu-Zn SOD and CAT decreased significantly. The activity of GSH-Px increased significantly. There was no significant difference among the activities of Mn SOD in all three groups. The level of MDA in DM group was much higher than that in NC or DT group. The relative expression levels of GSH-Px and Cu-Zn SOD mRNA in DM group were higher than those in other two groups, while the relative expression level of CAT decreased. Mn SOD mRNA was expressed without significant difference in all groups. Compared with NC or DT group, urinary protein in DM group increased significantly, while creatinine clearance rate decreased. CONCLUSIONS: Hyperglycemia affected the expression of antioxidant enzymes. Oxidative stress was caused by hyperglycemia in diabetic rats and may be an important factor in the etiology of diabetic nephropathy.     

铁皮石斛与钩状石斛杂交及种子无菌播种快繁研究

以铁皮石斛和钩状石斛为研究对象,通过人工辅助杂交获得杂交种子,诱导种子离体萌发,开展无菌播种技术研究.结果表明,铁皮石斛与钩状石斛杂交容易成功,杂交坐果率可达到80％;适宜的种子萌发培养基为MS+ 6-BA 1.0 mg/L+NAA 0.1 mg/L,培养30 d时的萌发率为89.7％;培养基MS+6-BA 1.5 mg/L+ NAA0.1 mg/L+香蕉100 g/L+ AC 1.0 mg/L对原球茎的增殖最好;培养基MS+ 6-BA 1.5 mg/L+ NAA 0.1mg/L+马铃薯200 g/L+ AC 1.0 g/L对原球茎的分化最好;壮苗生根培养基配方为1/2 MS+ NAA 0.6 mg/L+香蕉100 g/L+AC 1.0 g/L,植株生长健壮,生根率100％,移栽成活率达95％以上.     

70％丙森锌WP对黄瓜霜霉病的防效试验

摘要：针对不通浓度70％丙森锌WP进行黄瓜霜霉病的田间防效试验研究。结果表明：在黄瓜霜霉病发病初期,1hm2用70％丙森锌WP（有限成分）1911～2247g兑水900Kg,对黄瓜霜霉病有较好的防治效果。喷药间隔期为7d,共喷3次。药后7d各处理防效为81．94％一84．72％,对黄瓜生长安全。     

少花龙葵果总黄酮提取及抗氧化性研究

采用乙醇浸提法研究少花龙葵果中黄酮类物质的提取及其抗氧化性能。探讨溶剂浓度、温度、时间、料液比对黄酮提取的影响,并用正交试验优化了提取总黄酮的最佳提取条件。结果表明,最佳条件是:乙醇浓度为60%,提取温度为70℃,时间为2.5 h,料液比为1:12(g:mL)。此条件下测得总黄酮含量为3.68 mg·g-1,提取物对羟自由基有较好的清除效果。     

大白菜游离小孢子培养技术研究

游离小孢子培养技术是获得大白菜单倍体植株的有效途径之一。通过对影响大白菜游离小孢子胚胎发生的主要因素一材料基因型、供体母株生长状态、小孢子发育时期、培养方法、培养条件和影响植株再生频率的因素以及小孢子植株的加倍方法等进行了综述。     

荔枝上垫囊绿绵蜡蚧自然种群动态的研究

通过室内饲养观察和田间系统调查的方法，组建了垫囊绿绵蜡蚧Chloropuicinaria psidii Mask．自然种群生命表，研究了其在荔枝上自然种群动态发生趋势，结果表明，在自然条件下，垫囊绿绵蜡蚧的种群趋势指数为3．40；捕食性天敌对该蚧壳虫的种群具有一定的控制作用，其排除作用控制指数（EIPC）为2．414，“其他”类因子的EIPC为7.668，说明“其他”类因子是影响种群数量发展趋势的重要因子．