Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

毕节特产珍稀药材圆珠半夏的HPLC指纹图谱分析

为毕节大方圆珠半夏质量标准的建立提供参考,采用HPLC法对10批不同产地及不同采收时间的圆珠半夏进行了指纹图谱研究。结果表明:测定的10批半夏样品有23个共有峰,共有峰面积为91.6%~95.8%,相对峰面积的RSD为0.12%~0.40%;共有峰的相对保留时间从0.182~1.810min,相对保留时间的RSD在0.02%~0.10%。结论,该方法具有良好的稳定性和重复性,可用于圆珠半夏化学成分分析。     

水稻半外卷叶突变体sol1的表型分析与基因定位

适度卷曲有利于提高水稻叶片的光合效率,增加植株光合产物的有效积累量。我们利用甲基磺酸乙酯(EMS)处理籼型水稻保持系西农1B,获得一个稳定遗传的水稻半外卷叶突变体。该突变体从十叶期开始各叶片逐渐向外卷曲直至半卷状,并伴随茎秆半矮化和叶片披垂,暂被命名为semi-outcurved leaf 1(sol1)。与野生型(WT)相比,sol1的叶片卷曲指数均达到30%以上(P<0.01);倒一、倒二、倒三、倒四节节间长度和穗长极显著缩短,倒一、倒二、倒三叶的叶夹角显著或极显著增加;有效穗数、千粒重、每穗实粒数、结实率显著或极显著下降,一次枝梗数则增加11.3%(P<0.05)。sol1的蒸腾速率、胞间CO2浓度、气孔导度显著高于野生型。石蜡切片显示,sol1倒一叶的泡状细胞体积变小,数量显著增多,表皮细胞体积略微增大。遗传分析表明,sol1的半外卷叶性状受1对隐性核基因调控,定位于6号染色体标记JY6-3和JY6-10之间165 kb的物理范围内,共含15个注释基因。qRT-PCR结果表明,与泡状细胞相关的内卷基因和外卷叶基因RL14、Roc5、REL1在突变体sol1中呈不同程度的上调,NRL、BRD1、OsHox32、ADL1、LC2则呈不同程度的下调。研究结果为SOL1基因的克隆和功能研究奠定了基础。     

保护地蔬菜灰霉病菌的菌系分布及致病力分化测定

以山东省寿光市保护地栽培蔬菜上分离的30个灰霉病菌菌株为试材,采用菌落培养性状观察和菌饼活体接种法,研究灰霉病菌的菌系划分及对黄瓜叶片的致病力差异。结果表明:供试菌株划分为菌核型、菌丝型和孢子型3个菌系,不同菌株对黄瓜叶片的致病力分化显著,致病力强弱与寄主植物无显著相关性;强致病力菌株以菌核型为主,菌丝型和孢子型菌株致病力相对较弱。     

河南新县野生油茶群体表型变异特征研究

为揭示河南新县野生油茶群体的变异特征,以新县7个典型野生油茶生长区的群体为研究对象,测定了油茶叶、花、果相关的15个性状,采用变异系数、相关性分析、聚类分析和方差分析等方法,分析油茶表型性状的多样性及群体间和群体内的变异特征。结果表明,新县野生油茶叶、花、果形态在群体内及群体间的表型多样性均较为丰富,7个群体间叶、花、果形态差异均达到极显著水平。7个群体内15个性状中,鲜籽质量变异程度最高(49.79%),其次是鲜籽数、单果质量和果皮厚,变异程度最低的是叶长(10.67%);聚类分析在欧式平均距离在阈值15处,将新县野生油茶群体分为了两大类,在不同程度上反映了各群体的特征。     

文山州出口烟叶质量特点及可用性综述

阐述了英美烟草公司(BAT)与云南省文山州烟草合作历程,从烟叶物理质量特征、化学成分、感官评吸质量、安全性等方面介绍了文山州出口烟叶的质量特点及其等级与可用性,以供参考。     

产甲壳素酶菌株的筛选与诱变

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。     

考虑地震载荷作用的水电机组转动部件强度分析

近年地震多发,震中地区的水电机组均受到不同程度的损坏。为研究地震后机组运行的可靠性,根据恢复运行后其运行条件和方式较灾前变化情况,把承受转动部件的上机架纳入分析对象,对应不同工况在上机架上采用应变片测试来验证计算分析的正确性。为了准确计算转动部件承受的载荷,采用CFD对水轮机内部流场进行三维数值模拟,将流场的计算结果作为结构场的初始条件。以地震力和地震加速度两种方式加载地震载荷,采用单向流固耦合方法对各部件进行地震载荷下的仿真计算与工程实测数据对比发现二者吻合较好。结果表明,文章采用的方法能正确评估水轮机结构强度,对机组灾后运行安全性分析有一定指导作用。     

1株解淀粉芽孢杆菌B7快速液态发酵优化工艺研究

本试验以1株饲用益生菌解淀粉芽孢杆菌B7（Bacillus amyloliquefaciens B7，B.amyloliquefaciens B7）为研究对象，为了增强其在液态条件下的发酵水平，提高其芽孢发酵工艺优化效率，拟采用测定芽孢吡啶二羧酸（dipicolonic acid，DPA）浓度的方法来代替传统的平板芽孢计数法，以探讨此测定方法在芽孢发酵优化工艺中的可行性。首先，通过单因素试验筛选出对菌株产孢有显著促进作用的因子，进一步通过正交优化试验确定蛋白胨、玉米粉、大米蛋白粉和Mn²⁺组合的最优水平。结果表明，发酵液中的芽孢浓度与测定的DPA荧光强度呈线性相关，在蛋白胨20 g/L、玉米粉10 g/L、大米蛋白粉20 g/L、Mn²⁺1.0 mmol/L时发酵48 h，检测到DPA荧光强度达到最大，其相应的芽孢浓度达到7.0×10⁹ CFU/mL。与实验室常用的LB培养基相比，芽孢浓度提高了3.3倍；与工业生产用培养基相比，芽孢浓度提高了2.2倍。本研究为芽孢杆菌发酵过程中芽孢浓度测定提供了一种快速方法，为工业菌株B.amyloliquefaciens B7的发酵提供了一种成分简单且高产芽孢的培养基。