塔落岩黄芪、细枝岩黄芪及其变异型的生理特性研究

本文研究了毛乌素沙漠地区塔落岩黄芪和细枝岩黄芪及其变异型的生理学特性。结果表明,塔落岩黄芪和细枝岩黄芪各与其变异型之间在生理特性方面存在着极其显著的差异。     

科尔沁草地的畜草平衡与草业发展途径的研究

根据实地调查和历年的统计资料，系统分析研究了科尔沁草地多年来草业发展速度落后于牲畜增长速度、畜草供需严重失衡的现状。结果表明，草地退化４５．９％，超载率达３６．１％。该地区发展草业的自然和社会经济条件优越，但必须采取以下措施才能达到畜草平衡的目的：加强合理利用和改良天然草地；发展人工饲草料基地，迅速增加种植畜牧业比例；大力发展秸秆及农副产品饲草料加工业     

畜禽遗传资源保存的计算机模拟研究

运用计算机模拟方法产生畜禽品种的模拟保种群体,进行畜禽群体的保种研究。笔者考虑了选择、群体有效含量、性状的遗传力水平、控制性状的基因效应以及基因位点间的连锁强度等共24个参数组合,每种参数决定一个模拟群并且重复5次,共产生120个模拟群体,实验进行50世代。由模拟群体所得结果,讨论群体以及不同基因效应模型下群体生产性能均值、近交系数、基因丢失和群体基因频率等的变化,为实际畜禽保种工作提供理论依据。     

Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed

One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.     

桑蚕卵电晕人工孵化技术研究：V.电晕处理蚕种的农村饲育

通过１９９４～１９９６年电晕处理蚕种的农村饲养，得到以下几点认识，在保证卵面消毒和排除孵化不齐因素情况下，电晕蚕种与浸酸蚕种的产量质量无显著差异，专供农用的大板电晕仪存在放电不匀，孵经不齐和孵化率欠高的问题，有待改进，电晕处理蚕种还须进行卵消毒。     

Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.     

鸡的钙磷营养与科学利用

在现代养鸡生产中,钙磷饲料的科学利用是保证养鸡生产成功的关键问题之一,也是饲料生产企业非常关注的问题。如何根据鸡对钙磷营养需要的原理：在生产中科学地使用钙磷饲料应该引起重视。     

Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis

Feng, Q., Wu, G. H., Liang, T. M., Ji, H. Y., Jiang, X. J., Gu, W., Wang, W. Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis. J. vet. Pharmacol. Therap. 34 , 51–57. The purpose of this study was to investigate the hemolymph kinetics and depuration time of oxytetracycline following intramuscular administration at doses of 2, 8 and 40 mg/kg body weight, respectively. The concentration of OTC in hemolymph was assayed using solid phase extraction and high performance liquid chromatography. The elimination half‐life of the terminal part of the elimination phase (t_1/2β) ranged from 87.9 to 114.3 h. The total body clearance (CL_b) was 0.0430 L/kg/h at the lower dose, 0.0123 L/kg/h at the medium dose and 0.0013 L/kg/h at the higher dose. The apparent volume of the central compartment (V_c) was found to be 1.383, 0.699 and 0.143 L/kg respectively. The depuration time for each dose was 13.6, 29.6 and 57.6 days, respectively. Results from the present study suggest that the 40 mg/kg dose might have the best therapeutic efficacy following intramuscular administration.     

牛乳脂共轭亚油酸的合成及其营养调控

共轭亚油酸(CLA)是存在于动物脂肪和乳脂中的天然活性成分。本文综述了牛乳脂CLA的来源、合成机制及其营养调控。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.