辽西地区野兔危害情况调查

通过走访、问卷调查和样地调查,对辽西地区野兔危害情况进行了初步调查,并对调查数据进行了统计分析。结果表明：辽西地区野兔主要危害杏树,其次是刺槐、杨树和梨树等;野兔对不同林龄杏树危害差异显著。其中,对3林龄以下杏树危害严重,3林龄以上危害较轻;野兔对不同地区杏树危害程度差异显著,主要表现在对海拔较高降雨较少的地区危害程度较海拔低降雨相对多的地区高。     

浅谈规模猪场母猪子宫内膜炎的防治

近年来母猪子宫内膜炎在集约化养猪场的发病呈上升趋势,尤其是夏天高温季节,母猪产后发病率明显增加,有的养猪场发病率高达40％～60％。本文针对规模猪场的实际情况,提出一些切实可行的措施,为母猪子宫内膜内炎的治疗提供参考。     

Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis

Feng, Q., Wu, G. H., Liang, T. M., Ji, H. Y., Jiang, X. J., Gu, W., Wang, W. Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis. J. vet. Pharmacol. Therap. 34 , 51–57. The purpose of this study was to investigate the hemolymph kinetics and depuration time of oxytetracycline following intramuscular administration at doses of 2, 8 and 40 mg/kg body weight, respectively. The concentration of OTC in hemolymph was assayed using solid phase extraction and high performance liquid chromatography. The elimination half‐life of the terminal part of the elimination phase (t_1/2β) ranged from 87.9 to 114.3 h. The total body clearance (CL_b) was 0.0430 L/kg/h at the lower dose, 0.0123 L/kg/h at the medium dose and 0.0013 L/kg/h at the higher dose. The apparent volume of the central compartment (V_c) was found to be 1.383, 0.699 and 0.143 L/kg respectively. The depuration time for each dose was 13.6, 29.6 and 57.6 days, respectively. Results from the present study suggest that the 40 mg/kg dose might have the best therapeutic efficacy following intramuscular administration.     

甘蓝型油菜黄籽高含油量育种资源遗传多样性分析

以2份白菜型油菜为对照,用SRAP标记研究了57份甘蓝型油菜黄籽高油育种资源的遗传关系。在遗传相似系数0.682处,可将59份材料分为3类:两份白菜型油菜聚为一类;甘蓝型油菜除选系Y58单独聚为一类外,其他所有选系归为另一类,显示黄籽高油育种资源遗传基础较窄。在遗传相似性系数0.738处,又可将57份甘蓝型油菜分为6个亚类,其中53份材料归为两个大的亚类,系谱或亲缘关系较近的材料一般聚在同一亚类,且按系谱关系比地理来源划分明显,提示地理来源配制强优势黄籽高油杂交组合可靠性可能不如按系谱来源。     

Oral administration recombinant porcine epidermal growth factor enhances the jejunal digestive enzyme genes expression and activity of early-weaned piglets

This study attempted to determine ingested porcine epidermal growth factor (pEGF) on the gastrointestinal tract development of early-weaned piglets. Thirty-two piglets (14-day weaned) were randomly allotted to supplemented with 0 (control), 0.5, 1.0, or 1.5 mg pEGF/kg diet. Each treatment consisted of four replicates with two pigs per pen for a 14 days experimental period. Piglets were sacrificed and gastrointestinal tract samples were collected to measure mucosa morphology, mRNA expression and activities of digestive enzymes in the gastrointestinal tract of piglets at the end of the experiment. Diets supplemented with pEGF failed to influence growth performance but tended to increase jejunal mucosa weight (p < 0.09) and protein content (p < 0.07). Piglets supplemental pEGF induced incrementally the gastric pepsin activity (p < 0.05) and stimulated jejunal alkaline phosphatase (ALP) and lactase activities accompanied with the increase of jejunal ALP and maltase mRNA expression. No effect of pEGF on the activities of all enzymes in ileum except the stimulation of ileal aminopeptide N mRNA expression. These results reveal that dietary pEGF supplementation might enhance gene expression and activities of digestive enzymes in the stomach and jejunum of piglets.     

鲁梅克斯K-1杂交酸模和干玉米秸混合青贮料喂牛试验

８头４～５岁的役用秦川母牛按配对原则分为对照组和试验组。两组牛的精料配方基本相同，但试验组牛饲粮用７．７５％的鲁梅克斯Ｋ－１杂交酸模粉（以下简称鲁梅克斯Ｋ－１）代替对照组中同等重量的小麦麸；对照组牛的粗料全部用干玉米秸，试验组半用６０％干玉米秸和４０％的玉米秸＋鲁梅克斯叶混合青贮料。正试期２０ｄ。结果表明：试验组牛的平均日增重为 ０．７８７ｋｇ，对照组０．６９０ｋｇ，试验组较对照组牛日增重提高了 １４．０６％；每增重 １ｋｇ活重试验组牛消耗精、粗料分别为２．５４ｋｇ、５．２５ｋｇ，对照组半分别为２．９０ｋｇ、５．５０ｋｇ，试验组较对照组个节约精料０．３６ｋｇ、粗料０．２５ｋｇ；试验组每头牛毛盈利为７３．３４元，对照组５９．４０元，前者比后者提高经济效益２３．４７％。说明在役用母牛饲粮中，用鲁梅克斯粉代替精料中的小麦麸并用玉米秸＋鲁梅克斯混合青贮料能获得良好的生产性能和经济效益。     

发酵生产蛹虫草饲料添加剂的培养基优化

以多糖含量为发酵指标,通过单因素试验和正交试验对蛹虫草菌丝体发酵生产生物饲料添加剂的培养基组成进行优化。结果表明:最佳培养基组成为玉米粉4%,蚕蛹粉2%,MgSO40.04%,KH2PO40.15%,胞外多糖含量为10.14 g/L。     

8种豆科灌木栽培种丛枝菌根真菌种类及分布

采集人工栽培决明(Cassia tora)、假木豆(Dendrolobium triangulare)、紫穗槐(Amorpha fruticosa)、白刺花(Sophora davidii)、猪屎豆(Crotalaria pallida)、多花木蓝(Indigofera amblyantha)、木豆(Cajanus cajan)和银合欢(Leucaena leucocephala)8种豆科灌木植物根际5-10、10-20和20-30 cm 3个土层的土样,研究其根际丛枝菌根(AM)真菌的侵染率、孢子密度、种类及多样性。结果表明,8种豆科灌木植物AM真菌侵染率普遍较高,最高的为猪屎豆,80.8%;土壤速效磷含量与侵染率呈显著负相关(r=-0.733,P=0.039);根际土壤中的AM真菌孢子主要分布于5-20 cm土层,5-10 cm土层猪屎豆孢子密度最大,为115.33个·10 g-1土壤;宿主植物种类与土壤层位对孢子密度交互效应影响显著(F=100.497,P0.001);AM真菌优势种枫香球囊霉(Glomus liquidambaris)、脆无梗囊霉(Acaulospora delicate)在8种豆科灌木植物的土壤中均有分布。本研究表明,喀斯特地区适生豆科灌木植物具有丰富的AM真菌资源,今后可以在喀斯特地区开展豆科灌木植物接种优势AM真菌和根瘤菌等相关研究,这将对农牧业生产和退化生态系统修复效应的影响具有重要意义。     

季节性放牧对草地植物多样性与功能群特征的影响

针对放牧对高寒草地生态系统的影响,通过比较暖季放牧(6月-10月)和冷季放牧(11月-翌年5月)下典型高寒草地物种多样性和功能群特征的变化,分析了高寒草地植物群落对季节性放牧的响应机制。结果表明,暖季放牧草地中物种多样性指数为1.92,均匀度指数为0.78,明显高于冷季放牧草地的1.81和0.74,而冷季放牧草地的丰富度指数为17.45,高于暖季放牧草地的16.45。冷季放牧草地的地上生物量为246.61 g·m~(-2),莎草类和豆科类功能群的生物量比例分别为66.85%和15.86%,其值均高于暖季放牧草地。暖季放牧草地植物总密度为2 064株·m~(-2),明显高于冷季放牧草地的1 394株·m~(-2)。合理的放牧强度下,暖季放牧相比冷季放牧有利于草地群落物种多样性和均匀度的维持,但冷季放牧草地有利于草地群落地上生物量的积累。