20.67%万兴乳油对香蕉叶斑病的毒力测定与防治试验

香蕉尾孢菌叶斑病是危害香蕉的重要病害之一,在华南香蕉产区夏秋生长季节大量发生,严重危害香蕉叶片.2001～2003 年进行的新型复配杀菌剂20.67%万兴乳油对香蕉尾孢菌的毒力测定及对香蕉叶斑病的防治试验研究结果表明,20.67%万兴乳油对香蕉尾孢菌的抑制效果明显,对香蕉叶斑病防治效果优良,且对香蕉安全,施药3次的防治效果达81.23%～83.33%,优于对比药剂25%敌力脱乳油1 500倍的防效,是一种具保护兼治疗作用的高效杀菌剂,值得在香蕉生产上推广使用.     

橄榄基因资源保存库营建技术与效果分析

文章介绍了在茂名市国营八一林场营建橄榄基因库的方法及管理措施,并于2006年对基因保存库的成活率、生长量、结果株率、单株产果量、单位树冠投影面积产果量、果实性状等作了调查测定分析,结果表明:(1)采用苗圃嫁接成活苗上山造林及上山定砧后嫁接相结合的方法可加快橄榄基因库的营建;(2)基因库收集了广东主要橄榄栽培区7个县(市)11个农家品种81个优良单株,移栽及嫁接株共保存1 210株,保存率达95.65%,无性系结果比例达79.01%;(3)2002~2004年嫁接成活率为50.46%~80.05%,接株年均生长量树高0.7~1.0 m,径粗2.2~2.9 cm,冠幅0.8~0.9 m。总体结果株率、平均单株产果量和平均单位树冠投影面积产果量在嫁接4 a后分别为89.23%、1.11 kg和0.111 kg/m2,嫁接3 a后分别为69.61%、0.37 kg和0.054 kg/m2,嫁接2 a后分别为51.74%、0.15 kg和0.032 kg/m2;(4)橄榄单果质量4.63~12.64 g,果纵径2.63~4.00 cm,果形指数1.25~1.94,可食率78.51%~86.82%;(5)综合分析结果评选出g14、g18、g20、j1、p10、p8、g15、g2、g24、g27、x3、x4、x5、x6、g21、g32、g16、g33、g19、d15、d16、d7等22个为较优良无性系,其中又以g14、g18最优。     

粉单竹丰产栽培技术研究

通过对惠州市龙门县的粉单竹进行了丰产栽培试验,结果表明:立地条件、造林方式对粉单竹产量影响显著,在沿岸河滩地或村前屋后,采用埋节育苗造林方式造林,施腐熟粪肥为主,辅以适量复合肥对粉单竹的产量有明显提高.     

东北虎γ-干扰素基因的克隆、生物信息学分析及其在毕赤酵母中的表达

本试验旨在通过克隆东北虎γ-干扰素(IFN-γ)基因,研究其分子特征并预测蛋白生物学功能,为后续研究干扰素抗病毒活性做前期准备。通过RT-PCR从ConA诱导过的东北虎血淋巴细胞中扩增东北虎IFN-γ基因并测序,应用生物信息学方法进行序列分析。结果表明:东北虎IFN-γ编码区由504个核苷酸组成,共编码167个氨基酸,蛋白相对分子质量为19.59 ku,等电点为9.03,所编码的蛋白为碱性亲水性蛋白,其中前23个氨基酸可能为信号肽,IFN-γ编码蛋白保守结构域为IFN-γ超家族,且存在跨膜结构,其中1-6位氨基酸为胞内区域,7-28位氨基酸为跨膜区域,29-167位氨基酸为胞外区域;IFN-γ编码蛋白二级结构主要以α-螺旋(58.08%)和无规则卷曲(33.53%)为主,存在5个潜在的B细胞抗原表位,3个潜在的N-糖基化位点;分子进化分析显示,东北虎IFN-γ与GenBank上发表的东北虎、非洲狮、金钱豹、美洲狮、猎豹、家猫、加拿大猞猁、野猪等的核苷酸相似性为80.4%~99.8%,氨基酸相似性为70.5%~100%,东北虎IFN-γ与非洲狮、金钱豹亲缘关系最近,美洲狮、猎豹、家猫、猞猁次之,野猪最远。通过合成改造后的东北虎IFN-γ基因,构建能表达IFN-γ蛋白的重组质粒pPIC9K-IFN-γ,将其导入高效表达系统-毕赤酵母中进行诱导表达,经SDS-PAGE分析,表达蛋白的分子量约17.8 ku,与预期大小相符,表明东北虎IFN-γ成功表达。     

冰雪灾害后南岭常绿阔叶林受损优势种萌条特性

在南岭冰雪灾害受损常绿阔叶林建立4块样地,分析样地内受损树木的受损类型、径级、树种组成等因子与灾后萌条关系,结果表明:1)灾害后受损优势木72.00%以上均有萌条出现,乔木层优势木萌条率高于灌木层,为南岭地区冰雪灾害后受损森林生态系统快速恢复与重建提供保障;2)不同受损优势木萌条率存在差异,萌条率随着受损程度的增加呈增加的趋势,其中倒伏类型萌条率最高(100%),其他受损类型中灌木层金缕梅科萌条率最低,乔木层樟科萌条率最低;3)冰雪灾害后萌条特性表现出与采伐、刀耕火种后不同的规律,应做为1个特例进行研究;4)建议在我国亚热带地区森林自然干扰频发区,在人工促进恢复时多选用格药柃、小红栲和交让木等,有利于各层次结构与组成的保持.     

Driving forces of soil organic matter change in Jiangsu Province of China

Soil organic matter (SOM) is a key property determining soil functions and a major form of carbon stored in soil. Understanding the spatial and temporal variability of SOM and the driving forces responsible for spatial and temporal changes is important to assess regional soil quality and carbon sequestration potential and, particularly, to establish better practices for land use and management. We evaluated the spatio‐temporal change in SOM content from 1979–1982 to 2006 and its driving forces in Jiangsu Province, East China, using geostatistics. The results showed that mean SOM content increased from 16.60 ± 8.50 to 18.31 ± 8.32 g/kg over a 26‐yr period. The maps of SOM generated by ordinary kriging represented the increasing trend from north to south across the province in the two periods. The level of SOM in 1979–1982 affected the pattern of change: the SOM increasing in areas initially with a small content while decreasing in areas having a large content. The map of SOM change showed that the rate of increase decreased from north to south within the province. Increased fertilizer application promoted crop production with more residual biomass being retained in the soil, which resulted in increased SOM content. Land use changes to paddy, upland or forest improved SOM content, whereas abandoning land reduced SOM content.     

农业高校图书馆文献信息资源建设浅议——以湖南农业大学为例

分析了湖南农业大学图书馆馆藏文献信息资源的现状及增长情况,指出了存在的问题,提出了文献资信息源建设中应遵循科学性、实用性、系统性、特色性和效益性等基本原则,以确保馆藏文献信息资源的结构合理。     

微波消解和石墨炉原子吸收法测定水产品中的镉

采用微波消解系统处理样品,石墨炉原子吸收光谱法测定水产品中的镉,可以大大减少消化试剂的用量及样品的处理时间,干扰小,准确度高。镉的测定结果相对标准偏差为1.0%;检出限为0.002mg/kg。     

Effects of Neutral Phytase Supplementation on Biochemical Parameters in Grass Carp,Ctenopharyngodon idellus,and Gibel Carp,Carassius auratus gibelio,Fed Different Levels of Monocalcium Phosphate

Two 8‐wk studies were conducted to evaluate the effects of neutral phytase supplementation on hemato‐biochemical status, liver biochemical parameter, and intestinal digestive enzyme activity of grass carp, Ctenopharyngodon idellus, and gibel carp, Carassius auratus gibelio, fed with different levels of monocalcium phosphate (MCP). The control diet was prepared with 2% MCP but without phytase (P2.0). The other three experimental diets were prepared with the addition of 1.5, 1.0, and 0.5% MCP, respectively, when supplemented with 500 U/kg neutral phytase in each diet and designated as PP1.5, PP1.0, and PP0.5, respectively. The results indicated that the serum alkaline phosphatase (ALP), alanine transaminase (ALT), and aspartate transaminase (AST) activities, as well as the albumin (ALB) content were increased in grass carp (P < 0.05) and gibel carp (P > 0.05) fed with phytase‐supplemented diets. Meanwhile, the serum cholesterol, high‐density lipoprotein, and total protein contents of the two species of fish were increased in comparison to the control. In addition, dietary phytase inclusion did not significantly affect hepatic ALP, ALT, and AST activities in the two species of carp fed with different levels of MCP. Amylase activity increased in foregut and hindgut of both species when fed with the phytase‐supplemented diets while lipase activity was reduced in the foregut and hindgut in both fish. This study suggests that neutral phytase supplementation increases serum ALP, ALT, and AST activities but does not notably affect these enzyme activities in the liver of the two species of carp when fed different levels of MCP. On the other hand, amylase activity increased while lipase activity was reduced in the intestine of the species of carp fed with phytase‐supplemented diets.