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971.
Virus isolates GD6, GD7, GD8, GD9 and GD10 were obtained from Malvastrum coromandelianum showing leaf curl symptoms in Guangdong Province of China. A specific 500 bp product was consistently detected in total DNA extracts, amplified with universal primers specific for members of the genus Begomovirus. Analysis of their partial DNA sequences revealed that they are isolates of the same begomovirus species, sharing 92·8%–97·1% nucleotide sequence identity. The complete DNA sequences of both GD6 and GD9 were found to be 2767 nucleotides, with all the characteristic features of begomovirus genome organization. The two isolates have less than 85·2% nucleotide sequence identity with other reported begomoviruses. Consequently, GD6 and GD9 are considered to be isolates of a novel begomovirus species, for which the name Malvastrum leaf curl Guangdong virus (MLCuGdV) is proposed. Sequence analyses suggest that MLCuGdV may have arisen by recombination between viruses related to Papaya leaf curl China virus , Tomato leaf curl Philippines virus and other undiscovered virus ancestors. Neither the DNA-B component nor the DNAβ molecule associated with these begomovirus isolates was found. An infectious clone of GD6 was constructed. GD6 efficiently infected Nicotiana benthamiana , N. glutinosa and Petunia hybrida by agro-inoculation, and Malvastrum coromandelianum by whitefly transmission, inducing leaf curling, vein swelling and stunting symptoms. GD6 was also infectious in N. tabacum , but did not induce observable disease symptoms. 相似文献
972.
在实验室条件下,测定了甲霜灵和霜霉威复配剂对棉疫病菌(Phytophthora boehmeriae)的联合毒力。结果表明,复配剂MPA、MPB、MPC对棉疫病菌的DC5。分别为0.0555,0.1044μg/mL和0.0845μg/mL,对照单剂甲霜灵和霜霉威对棉疫病菌的EC50分别为0.0391μg/mL和10.6183μg/mL;联合毒力分析表明,甲霜灵和霜霉威按1:1.5复配有增效作用,按1:1和1.5:1复配有相减作用。 相似文献
973.
974.
犬类几种常见传染病诊断中基因芯片的应用 总被引:1,自引:0,他引:1
采用PCR技术扩增出犬腺病毒(CAV)ORF(P-VIII)基因、犬细小病毒(CPV)VP2基因、鸡血红蛋白的珠蛋白基因片断;采用RT-PCR扩增出犬冠病毒(CCV)纤突蛋白(S)基因、犬瘟热病毒(CDV)融合蛋白(F)基因、犬副流感病毒(CPIV)核衣壳结构蛋白(NP)基因、狂犬病病毒(RV)核蛋白(N)基因的各一段保守序列,纯化后点在硝酸纤维素膜上,制成基因芯片。通过RT-PCR将生物素标记到被检样品的核酸上,将已经标记的扩增产物与诊断基因芯片进行特异性的逆向点杂交,然后扫描仪对芯片进行扫描分析、判断。结果表明,此方法比病毒分离、HI、PCR方法灵敏度高、特异性强,平均检出率要高20%以上,并可同时对犬多种疫病进行快速诊断。 相似文献
975.
Aetiology of Rhizoctonia in sheath blight of maize in Sichuan 总被引:1,自引:0,他引:1
Rhizoctonia isolates obtained from maize grown in commercial fields in 33 representative counties (or cities) in Sichuan province in China were characterized according to colony morphology, hyphal anastomosis and pathogenicity. Of 141 isolates, 116 were identified as R. solani , 23 as R. zeae and two as binucleate Rhizoctonia . The isolates of R. solani were assigned to four anastomosis groups (AG): AG-1-IA (101 isolates, accounting for 71.6% of the total), AG-1-IB (2, 1.4%), AG-4 (9, 6.4%) and AG-5 (4, 2.8%). The two isolates of binucleate Rhizoctonia belonged to AG-K. On maize, isolates of AG-1-IA caused typical sheath blight symptoms. Lesions produced by isolates of AG-4, AG-5, AG-1-IB and AG-K were darker than those of AG-1-IA. Rhizoctonia zeae usually caused discontinuous lesions with a dark brown margin and a brown centre on the leaf sheaths, as well as ear rot. Isolates of AG-1-IA were the most virulent to maize, with an average lesion length of approximately 15 cm. Isolates of R. zeae produced lesions approximately 12 cm long, while those of AG-4, AG-5, AG-1-IB and AG-K were progressively shorter. On potato dextrose agar (PDA; pH 6.4), the minimum temperature for mycelial growth of R. zeae isolates was 14–18°C, the maximum 38–40°C and optimum 30°C. Isolates of R. zeae did not grow on PDA (28°C) at pH 2.0, the optimum for growth being pH 6.4. 相似文献
976.
977.
连续五年密植桃园的主干形修剪试验表明:主干形修剪技术用于密植栽培桃树早结果早丰产效果显著。‘春花’成苗定植后第二年产量达1300kg·666.7m-2,第三年为1500kg·666.7m-2,第四年为2250kg·666,7m-2,第五年为1750kg·666.7m-2;‘湖景蜜露’定植后第二年和第三年产量为1021kg·666.7m-2和1019kg·666.7m-2;‘锦绣’定植后第二年产量为120km·666.7m-2,第三年为1204km·666.7m-2,第四年为1876km·666.7m-2;‘沪463’定植后第三年产量为47.5kg·666.7m-2,第四年为2125kg·666.7m-2。主干形修剪夏季摘心可促进早期副梢的萌发,利用副梢坐果,均衡树势。主干形修剪对桃果实的品质有一定的影响,使果实相对变小,单果重减轻,要注意疏花疏果,加强肥水管理。 相似文献
978.
979.
喷克(mancozeb)80%可湿性粉剂600倍,800倍,1000倍室内对苹果轮纹(Physalosporapiricola)抑菌效果均达100%,600倍,00倍对苹果斑点落叶病菌(Alternariamali)炭疽病菌(Glomerellacingulata)亦具显著的抑制效果,人工接菌条件下,800倍液防治苹果斑点落叶病的实际保护期主10-12d,防治苹果轮纹病的有效保护期为8-10d。田 相似文献
980.