Therapeutic trial of granulocyte-colony stimulating factor for dilated cardiomyopathy in three dogs

Three dogs were presented to us for evaluation of cardiac problems. Electrocardiographic recordings revealed severe tachyarrhythmia and atrial fibrillation with ventricular tachycardia in 2 of the 3 dogs. The echocardiographic findings of the 3 dogs revealed markedly decreased fractional shortening and a marked increase in E-point septal separation. Based on the results of electrocardiographic and echocardiographic evaluation, the 3 dogs were diagnosed as dilated cardiomyopathy (DCM). The dogs were treated with conventional cardiac medication, but cardiac function did not improve and the clinical signs remained. We subsequently attempted treatment with granulocyte-colony stimulating factor (G-CSF; 10 microg/kg, subcutaneously). The specific purpose of G-CSF therapy for DCM was to improve cardiac function and a significant improvement in cardiac function was confirmed. The three dogs had no treatment side effects. This case report suggests that G-CSF might have therapeutic effects for medically refractory DCM in dogs.     

The effect of temperature on the energy budget of the Manila clam, <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>

In this study, the energy budget of the Manila clam, Ruditapes philippinarum, was evaluated after one-week acclimation periods at 5, 10, 15, 20, and 25°C. Small clams (151 ± 12 mg DW) and large clams (353 ± 16 mg DW) were fed with the microalgae, Isochrysis galbana. Filtration rate, ingestion rate, assimilation efficiency, oxygen-consumption rate, and ammonia excretion rate were measured. Both filtration rate and ingestion rate of small and large clams were found to be related to temperature. The highest Q ₁₀ values were measured in the range 15–20°C for both small and large clams. Assimilation efficiency of both small and large clams was not significantly influenced by temperature, although the maximum mean values were detected at 20°C. Oxygen consumption rate and ammonia excretion rate of small and large clams were found to be related directly to temperature over the entire range, with a maximum being detected at 25°C. The highest Q ₁₀ value was estimated in the range 10–15°C with regard to oxygen consumption rate, and in the range of 15–20°C with regard to ammonia excretion rate. Scope for growth (SFG) was positive at all temperatures, achieving a maximum value at 20°C in both small and large clams, primarily as a consequence of the enhanced ingestion rate which offset the concomitant elevation in the metabolic rate. In this study we have estimated the thermal optimum for this species at 20°C.     

禾谷镰刀菌产生的细胞壁降解酶的特性研究

禾谷镰刀菌(Fusarium gramimearum<\i>)是引起玉米茎腐病、穗腐病的主要病原菌之一。该病原菌在活体外能产生PG、PMG、PGTE和PMTE四种细胞壁降解酶,这些酶都具有酶的典型性动力学特性。细胞壁降解酶是F.gramimearum<\I>致病的一个重要因子。     

基于ETM+数据的新疆于田地区地表温度反演研究

地表温度是地球系统的热状况与水热平衡的关键因子.热红外遥感是获取地表热状况信息的一种非常重要的手段.利用ETM+数据和同时相气象数据获得两个地表温度反演的重要参数:大气透射率和地表比辐射率.运用覃志豪等的单窗算法反演出于田地区的地表温度,并用陆面实际地表温度进行了检验.结果表明,水体表面温度最低,绿洲次之,戈壁最高.地表温度以绿洲为中心从里到外呈环状分布.说明利用ETM+数据反演地表温度是可行的 .并对进一步理解我国西北干旱、半干旱区的土壤-植被-大气系统能量交换状况及区域热量分布差异有着重要意义.     

人HIF-1α基因重组减毒沙门氏菌载体的构建及稳定性检测

利用RT-PCR方法从低氧预处理的A549细胞中获得HIF-1α基因片段,将其定向插入pIRES-SEQ质粒NheⅠ、MLuⅠ2个酶切位点之间,得到真核表达载体pIRES-HIF-1α;采用电转化方法将其电转化入减毒沙门氏菌Ty21α中,获得重组减毒沙门氏菌菌株TPH;转染A549细胞,RT-PCR法检测HIF-1α基因表达,ELISA方法检测HIF-1α蛋白表达;TPH菌株在氨苄青霉素(+)和氨苄青霉素(-)LB培养板上分别传代40代,每10代提取质粒进行PCR及酶切鉴定.结果表明:试验成功构建了携带HIF-1α基因的重组减毒沙门氏菌TPH,其可在体外细胞中稳定表达HIF-1α基因及蛋白,TPH可稳定遗传.     

β-蒎烯合成诺蒎酸的反应条件研究

研究以松节油中的β-蒎烯为原料,通过KMnO4氧化合成诺蒎酸,并对氧化反应步骤中各因素对反应的影响进行了探讨。制备诺蒎酸的最佳条件为:β-蒎烯、KMnO4、NaOH的物质的量之比1∶2.5∶1,水为溶剂,温度25~30℃,反应时间4~5 h,得率达到35%。     

In vitro secretion of metabolic end-products by piscine haemoflagellates Cryptobia salmositica and C. bullocki (Kinetoplastida: Bodonidae) and the relationship of these products to the pH in the medium.

Pathogenic and nonpathogenic strains of Cryptobia salmositica Katz, 1951 and C. bullocki Strout, 1965 produced hydrogen peroxide, pyruvate and lactate under in vitro conditions in Minimum Essential Medium (MEM). As parasite number increased, the phenol red in the medium changed from red to yellow. This change was not associated with a decrease in pH, or an increase in pyruvate or lactate, but was correlated with an increased secretion of hydrogen peroxide. Parasites incubated at 10 degrees C in medium at pH 6.0, 6.5, 7.0 and 7.3 were active for about I week with decreasing activity in the absence of serum. Parasites in saline (pH 6.0, 6.5, 7.0 and 7.3) were nonmotile within 24 h and were dead in about 1 week. This suggests that these Cryptobia spp. are sensitive to changes in pH and require medium which is buffered, either with serum or Hepes.     

草地早熟禾的选育研究与利用

内蒙古畜牧科学院草原所自 1985年以来 ,对从荷兰、美国、瑞典等国家引进的草地早熟禾 11个品种和国内采集的野生种进行了引种驯化 ,筛选出了瓦巴斯、凯达布鲁克茅 2个品种适应内蒙古地区的气候特点 ,是适宜推广种植的优良品种。利用国内野生种采用单株选择方法培育出了大青山草地早熟禾品种 ,它较原始群体抗性增强 ,叶量增加。目前已有较大的推广利用面积     

Characterization of a feline homologue of the alphaE integrin subunit (CD103) reveals high specificity for intra-epithelial lymphocytes

The characteristics of a feline homologue of the alphaE integrin (CD103), defined by two murine monoclonal antibodies, Fe7.1B8 (IgG1) and Fe7.2D8 (IgG1), are described. These antibodies recognized 75% of intra-epithelial (range 59-88%) and 40% of lamina proprial (range 28-46%) T cells of the intestinal mucosal tissue of the small intestine in contrast with approximately 2% of peripheral blood lymphocytes. Both antibodies immunoprecipitated a 180 kDa protein from biotinylated feline intra-epithelial mucosal leukocytes consistent with the alphaE integrin subunit in conjunction with a 120 kDa protein consistent with the beta7 subunit. The nucleotide sequence of feline alphaE integrin, generated from molecular cloning of the feline alphaE encoding cDNA, is also reported. This feline molecule shares 72% sequence homology with human and 69% homology with murine and rat counterparts. Homology includes the presence of an X (extra) domain, that appears unique to alphaE molecules as described for human, rat and mouse, as well as areas of homology common to other alpha integrins. Of note is a typical I (inserted) domain, the presence of seven repeat regions, and highly conserved sequences in the cytoplasmic tail. Transfection studies demonstrated that both antibodies recognized an extracellular component which encompassed the X and I domains of the cloned alphaE integrin subunit. These studies demonstrate that the pattern of tissue distribution, biochemical characteristics, and cDNA sequence of the feline alphaE integrin subunit are largely similar to that described for other species.     

Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine

Characterization of the feline intestinal mucosal associated lymphoid tissue (MALT) will facilitate investigation of intestinal disease in the cat and promote the cat as an animal model for a range of human diseases which involve the intestinal lymphoid tissue. This includes inflammatory bowel disease, viral and non-viral associated intestinal lymphomas and immunodeficiency associated syndromes. Morphologic and phenotypic characterization of the normal small intestinal diffuse MALT in 22 SPF cats was performed using flow cytometry and cytology on isolated intestinal leukocytes from the intra-epithelial and lamina proprial compartments, as well as immunohistology on tissues from the feline duodenum, jejunum and ileum. The intra-epithelial compartment (IEC) was dominated by lymphocytes (>85%) which frequently contained intracytoplasmic granules. The most striking findings in the IEC were the elevated percentages of CD8 alpha+ lymphocytes (40%), presumed to express CD8 alpha alpha chains, and CD4-/CD8- (double negative) lymphocytes (44%), and the consistent presence of a minor subpopulation of CD3+/CD11d+ IELs (6%). Small percentages of CD4+ lymphocytes (10%) were observed such that the IEL CD4:CD8 ratio (0.25) was low. The LPC also contained a majority of T cells and few plasma cells. However, this compartment had reduced percentages of CD8 alpha+ lymphocytes (28%) and increased percentages of CD4+ lymphocytes (27%) relative to the IEC. However, the LPL CD4:CD8 ratio (1.0) remained low compared with the ratio in peripheral blood. In feline MALT, MHC class II expression was lower than in other peripheral lymphoid compartments. The results of this study provide important reference values for future investigations involving feline intestinal lymphocytes and demonstrates that the leukocyte distribution and phenotypic characteristics of the feline diffuse MALT appear largely similar to the murine, rat and human counterparts.