全文获取类型
收费全文 | 657篇 |
免费 | 51篇 |
国内免费 | 3篇 |
专业分类
林业 | 28篇 |
农学 | 13篇 |
基础科学 | 2篇 |
159篇 | |
综合类 | 147篇 |
农作物 | 25篇 |
水产渔业 | 37篇 |
畜牧兽医 | 261篇 |
园艺 | 6篇 |
植物保护 | 33篇 |
出版年
2023年 | 4篇 |
2021年 | 10篇 |
2020年 | 13篇 |
2019年 | 15篇 |
2018年 | 13篇 |
2017年 | 13篇 |
2016年 | 21篇 |
2015年 | 22篇 |
2014年 | 18篇 |
2013年 | 29篇 |
2012年 | 30篇 |
2011年 | 32篇 |
2010年 | 28篇 |
2009年 | 16篇 |
2008年 | 26篇 |
2007年 | 24篇 |
2006年 | 23篇 |
2005年 | 35篇 |
2004年 | 27篇 |
2003年 | 19篇 |
2002年 | 19篇 |
2001年 | 22篇 |
2000年 | 23篇 |
1999年 | 21篇 |
1998年 | 18篇 |
1997年 | 12篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 6篇 |
1993年 | 10篇 |
1992年 | 12篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 13篇 |
1988年 | 9篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 12篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 3篇 |
排序方式: 共有711条查询结果,搜索用时 250 毫秒
101.
102.
TAN Wan-qin Phang Chiun Yee Sieo Chin Chin Yiap Beow Chin Clemente Michael Wong Vui Ling Norhani Abdullah Son Radu Ho Yin Wan 《农业科学学报》2015,14(9):1816-1826
The full length phytase gene of Mitsuokella jalaludinii was successfully cloned and was found to be 1 047 bp in length, with 348 amino acids, and was designated as PHY7 phytase gene. A comparison of the sequence of PHY7 phytase gene of M. jalaludinii with various microbial phytase gene sequences showed that it was not similar to those from other bacteria except Selenomonas ruminatium, thus suggesting that they may both express a new class of phytase. The PHY7 phytase gene was subsequently subcloned into bacterial expression vector, p ET32 a, for expression in Escherichia coli strain Rosetta-gami. Expression of the recombinant phytase gene was optimised and characterised. The recombinant phytase was estimated to be approximately 55 k Da by SDS-PAGE analysis. The recombinant phytase exhibited optimum activity at 55°C, p H 4.5 and showed good p H stability from p H 3.5 to 5.5(78% relative activity). Metal ions such as Ca2+, Mg2+, and K+ were found to exert significant stimulatory effect on the recombinant phytase activity while Cu2+, Fe3+, and Zn2+ greatly inhibited the enzyme activity. The recombinant phytase showed moderate resistance to trypsin proteolysis, but susceptible to pepsin proteolysis. The results of the study showed that several characteristics of recombinant phytase were slightly different from the native enzyme. Unfavourable characteristics such as reduced p H stability and metal ion effects should be taken into consideration during feed enzyme formulation. 相似文献
103.
Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures,long analysis time,large instruments and professional personnel to meet the increasing demands. In this review,we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents,toxic ions,pesticides,veterinary drugs and preservatives,as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid,accurate,easy-to-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system. 相似文献
104.
105.
Real-time ultrasound imaging was used in a clinical study to estimate the number of follicles of different sizes, ovulation and conception rates, and to study follicle dynamics following oestrus-induction of bitches.
Follicles were identified during late anoestrus (between 100 and 60 days prior to the pre-ovulatory LH surge) and there appeared to be a shift in the population from small follicles (1–3 mm in diameter) to large follicles (>4 mm diameter) approximately 2 days prior to the LH surge. Corpora lutea could be reliably identified although the majority were cavitated. High ovulation rates (97–100%) and pregnant rates (86–100%) were detected, and although the conception rate was approximately 70% it varied between 8 and 92%. Within the narrow range of the clinical population studied there were trends relating age to reproduction performance. Oestrus induction with a gonadotrophin regime appeared to result in large numbers of small follicles that did not ovulate, whilst when using cabergoline the number of small and large follicels and the number of copora lutea were similar to those of control cycles. 相似文献
Follicles were identified during late anoestrus (between 100 and 60 days prior to the pre-ovulatory LH surge) and there appeared to be a shift in the population from small follicles (1–3 mm in diameter) to large follicles (>4 mm diameter) approximately 2 days prior to the LH surge. Corpora lutea could be reliably identified although the majority were cavitated. High ovulation rates (97–100%) and pregnant rates (86–100%) were detected, and although the conception rate was approximately 70% it varied between 8 and 92%. Within the narrow range of the clinical population studied there were trends relating age to reproduction performance. Oestrus induction with a gonadotrophin regime appeared to result in large numbers of small follicles that did not ovulate, whilst when using cabergoline the number of small and large follicels and the number of copora lutea were similar to those of control cycles. 相似文献
106.
Acute toxicity in five dogs after ingestion of a commercial snail and slug bait containing iron EDTA 总被引:1,自引:1,他引:0
This case series of five dogs describes the effects of ingesting large amounts of an iron EDTA snail-bait product. In all cases signs of toxicity occurred between 6 and 24 h after ingestion and included abdominal pain and haemorrhagic gastroenteritis. Two of the dogs had pretreatment serum iron levels measured and in both cases the levels were above normal limits. All of the dogs were treated with iron chelation therapy and supportive care including intravenous fluids, analgesics, gastric protectants and antibiotics. Chelation therapy with desferrioxamine mesylate did not cause adverse effects in any of the dogs and all survived to discharge. The effects of iron EDTA snail bait in dogs requires further study and minimum toxic doses need to be established. 相似文献
107.
Clemens M. Altaner Elena N. Tokareva Janet C. T. Wong Adrian I. Hapca J. Paul McLean Michael C. Jarvis 《Wood Science and Technology》2009,43(3-4):279-290
As the severity of compression wood influences the mechanical and chemical properties of wood it is desirable to be able to measure compression wood severity. However, so far no satisfactory method has been reported in the literature. Here we describe how scanning FTIR micro-spectroscopy can be employed to achieve CW severity measurements on increment cores of Norway spruce (Picea abies (L. Karst.) and Sitka spruce (Picea sitchensis (Bong.) Carrière). Radial wood strips were converted into sawdust by a process that maintained their spatial orientation. Samples prepared in this way were scanned with an FTIR-microscope in reflective mode and from the spectra obtained a CW-indicator was calculated representing aromatic and carboxyl signals. This FTIR CW-indicator correlated well with alternative CW identification techniques (namely microfibril angle, transmitted light and immunolabelling of beta 1–4 galactan), which have been used to validate the method. Repeatability of the measurements was good and no systematic difference between spruce species was found. The achievable resolution of the measurements was of sub-mm order. The CW indicator described offers the opportunity to correlate CW severity with mechanical wood properties in spruce. 相似文献
108.
Lawrence W. C. Lai K. W. Chau S. K. Wong N. Matsuda Frank T. Lorne 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):289-316
ABSTRACT From a transaction cost perspective, this paper shows how the tradition of consuming marine fish by the majority of Hong Kong's Chinese citizens has shaped the means and modes of marketing seafood in Hong Kong. It is argued that consumer preferences stimulate aquaculture as a non-open access measure to bypass state fish marketing regulations. This has resulted in two outcomes. First, the transaction cost savings on metering output quantity and quality under private property have led not only to the collapse of the state monopoly on marine fish marketing, but also to the emergence of the (then new but) currently popular kind of Chinese seafood restaurant in Hong Kong. Second, consumer preferences for variety have shaped the form of coordination between the producer and the consumer. The advantage of market coordination has led to the emergence of a system of wholesaling and retailing rather than vertical integration. Issues concerning the emergence of Hong Kong as a regional live marine fish production and trading centre are discussed in terms of the contribution of local mariculture to sustainable development. 相似文献
109.
K. L. Hepworth‐Warren B. L. Hay Kraus D. M. Wong A. C. Krull G. L. Metcalf 《Equine Veterinary Education》2017,29(11):603-608
This report describes an 11‐year‐old Percheron mare that presented with signs of colic and was ultimately diagnosed with bacterial peritonitis of unknown origin. Bacterial culture of a peritoneal fluid sample isolated 2 Clostridial species, one of which was strongly suspected to be Clostridium haemolyticum. The horse was markedly hypoalbuminaemic at presentation, leading to the development of low oncotic pressure and ventral oedema. The mare was administered a low molecular weight/low molar substitution hydroxyethyl starch solution in conjunction with other therapies that resulted in marked improvement of clinical signs. The purpose of this report is to describe the clinical findings associated with equine peritonitis associated with C. haemolyticum, a rarely identified pathogen in the horse. Secondly, this report serves to describe the beneficial effects of tetrastarch administration in a clinical case with severe hypoalbuminaemia and ventral oedema. 相似文献
110.
This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. (UPMP3) and Burkholderia sp. (UPMB3) to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 (FocR4). Increased accumulation of resistance-related enzymes such as peroxidase (PO), phenylalanine ammonia lyase (PAL), lignithioglycolic acid (LTGA), and pathogenesis-related (PR) proteins (ehitinase and β-1,3-glucanase) has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana. 相似文献