Population declines of a long-lived salamander: a 20+-year study of hellbenders, Cryptobranchus alleganiensis

Accurate assessment of whether long-lived species are stable or declining is challenging. Life history characteristics such as delayed maturity result in relatively slow population responses to perturbations, so data should be collected across a relatively long time span. Because differential effects on age classes can be important, studies should also examine potential changes in the population's age structure. Moreover, multiple populations should be studied to indicate whether changes are regional or are restricted to local populations. We incorporated all three factors (long duration, multiple populations, age structure data) into our study of the conservation status of a long-lived aquatic salamander, the hellbender, Cryptobranchus alleganiensis. Over the 20+ years of this study, populations of hellbenders declined by an average of about 77%. This decline was characterized by a shift in size (age) structure, with a disproportionate decrease in numbers of young individuals. The change in density and age structure was consistent for populations in five rivers and for two subspecies (C. a. alleganiensis and C. a. bishopi), indicating that the decline is not restricted to one or two local populations. For the population with the most extensive data, the decline had clearly begun by the 1980s and there was a significant decrease in body condition over the period of the study. It is not known whether population declines for hellbenders have a single cause or whether each population has experienced independent declines.     

GRAZING BEHAVIOUR AND WEIGHT CHANGES IN CALVES TURNED OUT TO PASTURE

Two studies are described in which the behaviour of 24 seven-month-old Friesian steer calves was observed. The first study (G1) was undertaken at the time of turnout to pasture, and the second (G2) 5 days later. The calves appeared to adapt rapidly to the change in environment, though in study G1 grazing was frequently interrupted. In study G2 the calves showed normal behaviour patterns and spent a total of 4.25 hours grazing. There was a positive correlation between age and weight at turnout and time spent grazing in study G1. However, this relationship was not apparent in G2, and there was no relationship between time spent grazing in G1 and G2. Mean weight loss upon turnout was 7.44% of live weight, in spite of the feeding of hay and barley at grass. There was no relationship between weight at turnout and subsequent weight loss. The results confirmed other experimental findings which suggest that weight loss at turnout is largely a reflection of changes in the weight of alimentary tract contents, rather than an inability to adapt to the change in environment. The calves were grazed at two intensities (‘low’ and ‘standard’) during the grazing season. Growth rate in winter and weight at turnout were negatively correlated with growth rate in the first month of the grazing season. This relationship was more apparent in the ‘low’ intensity group which received a liberal daily herbage allowance at this stage of the season. Partial correlation analysis, in which pre-grazing growth rate was held constant, indicated that weight and age had little independent influence on growth rate at grass, though there was a positive relationship between weight for age at turnout and gain during the grazing season. The importance of a target weight at turnout is discussed in relation to the 18-month beef production system. It is concluded that younger, lighter, cattle at turnout may not be at a disadvantage when adequate amounts of herbage are on offer early in the grazing season.     

CDAA inhibition of Kaurene oxidation in etiolated sorghum coleoptiles

N,N-Diallyl-2-chloroacetamide (CDAA) (0.25 ppmw; I μM) inhibited growth of 14-day-old sorghum (Sorghum bicolor (L) Moench. cv Funks G 522DR) when the herbicide was incorporated into sand. Kaurene oxidation was inhibited in a cell-free enzyme preparation from 4-day-old unruptured, etiolated coleoptiles. CDAA (1 μM) inhibited incorporation of [¹⁴C]mevalonic acid into kauren-19-oic acid (50%), with resultant increases in concentration of precursors. Thus, inhibition of gibberellin precursor biosynthesis was demonstrated, and this activity would explain many of the morphogenic and biochemical responses of grasses to CDAA.     

Cutaneous metastasis of a colonic carcinoma in a dog

An entire seven-year-old Afghan bitch was referred with a two month history of pruritic, erythematous and ulcerated lesions involving the skin of the ventral abdomen, caudal mammae, inguinal region and the inner thighs. Other clinical signs included pyrexia, mild dyspnoea, mammary hypertrophy and lactation. A skin biopsy confirmed an anaplastic carcinoma. At necropsy, the neoplasm was located in the colon, urinary bladder, liver, peritoneal wall, diaphragm, parietal pleura and mediastinal membranes, including the pericardial sac attachment. The colon was considered to be the primary site.     

Evaluation of the morantel sustained release bolus in cows and calves

The efficacy of the morantel sustained release bolus (MSRB) in controlling gastrointestinal parasitism in beef cattle was assessed during the 1982 spring-autumn grazing season. Forty-eight cows and their calves were allotted to three equal groups. One group (T-1) served as a nonmedicated control group. One MSRB was administered to each calf of the T-2 group, and to each cow and calf of the T-3 group at the beginning of the study. The efficacy of the bolus was assessed by comparison of weight gain performance and parasitological data (fecal worm egg counts, herbage larval counts, worm counts from tracer and principal trial calves, and plasma pepsinogen level determinations). Though not statistically significant, treated calves from Group T-2 had a numerical mean weight gain advantage of 2.6 kg, and those from Group T-3 of 4.7 kg, over control calves. Average daily gains (ADG) for the three groups of calves were 0.69, 0.72, and 0.73 kg, respectively. Untreated cows from Group T-2 and treated cows from Group T-3 outperformed the control cows by 12.3 and 7.5 kg, respectively. Fecal worm egg counts from both groups of treated calves were significantly (P less than 0.01) lower than counts from control calves during the entire 169-day trial; notably, egg counts were reduced by 99% 28 days after MSRB administration to both groups of calves. There were no significant differences in the number of eggs counted from the three groups of cows, probably because of the very low numbers of eggs encountered. Mean total worm burdens of principal calves (six per group) necropsied at trial termination indicated a 91% (P less than 0.01) reduction in Group T-2 and an 87% reduction (P less than 0.01) in Group T-3. Worm-free tracer calves were introduced onto pastures every 28 days to monitor availability of infective larvae. The mean number of worms recovered at necropsy from tracer calves that grazed with control cattle increased as the season progressed. However, the numbers of parasites recovered each month from mid-August through mid-October from tracers that grazed pastures with treated cattle were lower (P less than 0.05) than those levels displayed at trial initiation. In addition, the mean numbers of worms from treated group tracers were lower than from the controls for each necropsy period.(ABSTRACT TRUNCATED AT 400 WORDS)     

Metabolism of [C]vernolate in soybeans

Penetration and metabolism of [¹⁴C]vernolate in soybean [Glycine max (L.) Merr. var Ransom] pods and seeds were measured 0, 1, 4, 24, 48, or 72 hr after treatment which occurred at 40 days after flowering. Total ¹⁴C recovery decreased ca. 50% within 4 hr and the loss of ¹⁴C was considered to be a measure of volatility. Total nonpolar extractants decreased in a logarithmic pattern which approached 10% of total ¹⁴C recovered within 24–48 hr. Total polar extractants increased in a logarithmic pattern to a maximum of 90% of total ¹⁴C recovered within 24 hr. Seed nonpolar extractants never exceeded 2% of the total ¹⁴C recovered while pod nonpolar extractants consisted of vernolate plus an unidentified component that did not thin-layer chromatograph (TLC) as the sulfone or sulfoxide. Pod polar extractants increased with time to ca. 75% of the total ¹⁴C recovered (24–48 hr) and decreased to ca. 58% at 72 hr after treatment. Seed polar extractants averaged ca. 10% of total ¹⁴C recovered for the first 48 hr after treatment and then increased to 30% of total ¹⁴C recovered. Thus, [¹⁴C]vernolate per se concentration decreased to <1% of applied material within 72 hr through volatilization and degradation of nonpolar extractants to polar products. Polar metabolites showed two major patterns of vernolate detoxification. One detoxification system produced ¹⁴C-metabolites whose R_f's were equivalent to that reported in corn (Zea mays L.) [J. P. Hubbell and J. E. Casida, [J. Agric. Food Chem. 25, 404 (1977)] and accounted for <30% of the pod polar extractants. A second detoxification system was most prevalent in soybean pod and seed tissues and resulted in very rapid modification of vernolate with an unidentified product that was 85% of the extracted ¹⁴C within 4 hr after treatment and which decreased in concentration with time. Therefore, unexplained vernolate detoxification system(s) exist in soybean pod and seed.