兰州大尾羊心脏型脂肪酸结合蛋白（H-FABP） 基因克隆及其同源性比较

【目的】克隆兰州大尾羊心脏型肪酸结合蛋白（H-FABP）基因全长cDNA序列,为研究绵羊H-FABP生物学作用和生产应用提供理论依据。【方法】根据已知哺乳动物H-FABP基因 cDNA 序列,设计5''和3''特异引物,运用cDNA 末端快速扩增（RACE）技术获得兰州大尾羊H-FABP基因全长 cDNA 序列。【结果】 扩增获得兰州大尾羊5''端425 bp、3''端231 bp片段和 177 bp中间片段,拼接获得748 bp兰州大尾羊H-FABP基因全长cDNA 序列（GenBank登录号：JQ780322）。 兰州大尾羊H-FABP基因ORF长 402 bp,编码 133 个氨基酸。核苷酸序列分析显示兰州大尾羊H-FABP基因序列与大多数哺乳动物相似,但其第66位发生的碱基转换（T←→G）引起所编码的第22位天门冬氨酸（N）不同于其它所有物种的赖氨酸（K）。构建的基因进化树分析结果显示兰州大尾羊与山羊亲缘关系最近。预测兰州大尾羊H-FABP蛋白质的空间结构与山羊和牛H-FABP类似,由2个α螺旋和10个反向平行的β折叠组成,10 个折叠片围成一个桶状结构,疏水性残基位于桶内,用于结合脂肪酸。【结论】克隆了兰州大尾羊H-FABP基因,为进一步研究该基因的功能奠定了基础。     

A Parametric Transfer Function for Grain-Flow Within a Conventional Combine Harvester

Grain yield monitoring is an integral tool in the Precision Agriculture management system. When used in conjunction with a satellite-based navigation system, it provides spatial information on output variability, output response to managed inputs and is used to identify limiting resources in the crop production process. Accurately matching measured yield quantities with spatial units within a field is therefore important. At present, a simple linear time shift is employed by all commercial monitoring systems to account for the delay between GPS recorded positions and subsequent yield measurements. This study examines the internal process of grain transport to the sensor by monitoring the flow of strategically coloured grain. The flow is shown to be significantly influenced by mixing induced by threshing and auger transport processes. In contrast to the common assumption that grain moves as a spatially related cohort through to the sensor, the results suggest that a diffusion process is more realistic. A parametric model for the diffusion process is provided which suggests that from each individual yield measurement a maximum 20% of the mass could be assigned to a single spatial unit of the size that is typically allocated. The results imply that for further analyses, the inconclusive spatial origin and artificially smoothed quantities of instantaneous yield measurements should be considered.     

不同干豆豆浆机制备豆浆的主要品质评价

以大豆为原料选用我国2个主导品牌的3款豆浆机(九阳精磨豆浆机、九阳无网干豆豆浆机、美的无网干豆豆浆机)制作豆浆,从蛋白质含量、蛋白质回收率、豆浆颗粒度及感官特性等方面对豆浆品质进行评价.结果表明:豆浆的蛋白含量为1.72%～2.66%,蛋白回收率为47.13%～68.66%,带有精磨器的豆浆机制备的豆浆蛋白含量及蛋白回...     

Approaches to pathogen-mediated resistance breeding against plum pox potyvirus in stone-fruit trees1

In a programme for developing systems which allow the transfer of foreign genes into apricot cultivars, we have tested cotyledons of immature embryos, somatic embryos and leaf discs. Apricot plants have been transformed, and then regenerated, with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids: pBinGUSint, carrying the marker gene β-glucuronidase (GUS), and pBinPPVm, carrying the coat-protein gene of plum pox potyvirus (PPV). The marker gene GUS was used for visual evaluation of the efficiency of the transformation system. The coat-protein gene was used in the hope of introducing coat protein-mediated resistance to one of the most important stone-fruit pathogens in Europe and the Mediterranean area.     

In vitro analysis of antiangiogenic activity of fungi isolated from clinical cases of equine keratomycosis

OBJECTIVE: The goal of this project was to explore the possibility that fungal organisms produce metabolites that inhibit angiogenesis. Procedures Fungal cultures were obtained from cases of keratomycosis, grown in Sabouraud's dextrose broth, and sterile filtered for use in experiments. The Matrigel assay was used to screen the filtrate samples for antiangiogenic activity. Matrigel is a basement membrane matrix that supports the differentiation of human umbilical vein endothelial (HUVE) cells into a capillary-like network of tubules. HUVE cells were cultured using standard techniques and passaged at confluence, with all cells being used at passage 3-6. HUVE cells (40 000 cells) were pipetted into each well of a 24-well tissue-culture plate coated with Matrigel. An aliquot of fungal media filtrate was added to each well and the plates allowed to incubate for 18 h, at which time they were evaluated for tubule formation. RESULTS: Two fungal isolates showed inhibition of tubule formation. The addition of 100, 200 and 400 &mgr;L of the fungal media filtrate from the first isolate (Fusarium sp. 99A34574) produced a consistent and dose-dependent inhibition of tubule formation. The second isolate (Aspergillus sp. 271599) did not show inhibition of tubule formation with 100 or 200 &mgr;L added to the wells, however, it did show inhibition at 400 &mgr;L/well. The remaining three isolates did not cause inhibition at any concentration. CONCLUSIONS: Our findings suggest that certain fungal organisms produce metabolites that inhibit tubule formation in vitro, and that these metabolites may play a significant role in altering the host vascular response to fungal infections of the cornea.     

内蒙古阿拉善盟草原疯草危害调查

采用现场调查和资料收集相结合的方法，对内蒙古阿拉善盟的阿拉善左旗、阿拉善右旗和额济纳旗3个旗疯草的种类、分布、危害及防治对策进行了调查研究。结果表明：阿拉善盟有疯草7种，其中棘豆属有毒植物3种，黄芪属有毒植物4种，分布面积超过139．33万hm^2，危害最严重的是小花棘豆和变异黄芪，给当地畜牧业造成了极大的危害，并就如何综合利用疯草提出了几点措施。     

浙江天童国家森林公园常绿阔叶林主要组成树种材积与生物量相关关系探讨

以天童国家森林公园常绿阔叶林主要组成树种为研究对象,通过测定其各组分生物量,建立了实测生物量与材积之间的回归模型,结果表明:主要组成树种各组分生物量与其材积之间存在着密切的相关关系,各主要树种材积与生物量的回归模型存在差异;回归方程精度较高,用于根据地区常绿阔叶林主要树种材积推算其生物量是可行的。     

氧化塘处理原木蒸煮废水

介绍了原木蒸煮废水的处理方法,提出氧化塘法是小型木材加工厂处理该废水的最佳选择,并用实例简单介绍了氧化塘的设计选型问题.