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151.
E. F. Boys S. E. Roques A. M. Ashby N. Evans A. O. Latunde-Dada J. E. Thomas J. S. West B. D. L. Fitt 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(4):307-321
Light leaf spot (Pyrenopeziza brassicae) is an important disease on winter oilseed rape crops (Brassica napus) in northern Europe. In regions where economically damaging epidemics occur, resistance to P. brassicae in commercial cultivars is generally insufficient to control the disease without the use of fungicides. Two major genes for
resistance have been identified in seedling experiments, which may operate by decreasing colonisation of B. napus leaf tissues and P. brassicae sporulation. Much of the resistance present in current commercial cultivars is thought to be minor gene-mediated and, in
crops, disease escape and tolerance also operate. The subtle strategy of the pathogen means that early colonisation of host
tissues is asymptomatic, so a range of techniques and molecular tools is required to investigate mechanisms of resistance.
Whilst resistance of new cultivars needs to be assessed in field experiments where they are exposed to populations of P. brassicae under natural conditions, such experiments provide little insight into components of resistance. Genetic components are best
assessed in controlled environment experiments with single spore (genetically fixed) P. brassicae isolates. Data for cultivars used in the UK Recommended List trials over several seasons demonstrate how the efficacy of
cultivar resistance can be reduced when they are deployed on a widespread scale. There is a need to improve understanding
of the components of resistance to P. brassicae to guide the development of breeding and deployment strategies for sustainable management of resistance to P. brassicae in Europe. 相似文献
152.
This study investigated the physical state and metabolic status of lambs of different birth ranks soon after birth. The aim was to identify the factors that result in poorer survival rates in triplet than single or twin lambs. Six hundred and twenty one Romney ewes were mated over a 4-day period and 46 of them were observed continually from day 143 of pregnancy until they finished lambing. Blood samples, taken from all lambs within 5 min of birth, were placed on ice, centrifuged and the plasma harvested and subsequently analysed for glucose, fructose, lactate, thyroxine (T4) and gamma-glutamyl transferase (GGT). The rectal temperature of each lamb was recorded at birth and at 1, 2, 3 and 6 h of life. Lambs were weighed and measured at 3 h of age.At birth, triplet lambs were significantly lighter (P < 0.05) and had lower (P < 0.05) plasma fructose and thyroxine than twin and single lambs, higher (P < 0.05) lactate concentrations and a lower rectal temperature (P < 0.05) than twin lambs. These observations suggest that they are subject to placental insufficiency. The packed cell volume was significantly (P < 0.001) higher in twin than triplet lambs. The order at birth of twin or triplet lambs had no effect on birthweight or plasma metabolite concentrations. The smallest triplet was significantly lighter (P < 0.05) and had higher plasma lactate (P < 0.05) concentration than the largest triplet.These physiological limitations found in smaller triplet lambs at birth leave them prone to perinatal mortality. Management techniques, to increase triplet lamb birthweight and thereby improve their survival outcome need further investigation. 相似文献
153.
154.
Yong-Ju?HuangEmail author Bruce?D.?L.?Fitt Malgorzata?Jedryczka Sylwia?Dakowska Jonathan?S.?West Peter?Gladders Julie?M.?Steed Zi-Qin?Li 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(3):263-277
Experiments over five growing seasons at Rothamsted (1998/99–2002/03), four seasons at Boxworth (1998/99, 1999/2000, 2001/02, 2002/03) in England (Leptosphaeria maculans) and three seasons (1998/99–2000/01) at Poznan in Poland (Leptosphaeria biglobosa) suggest that differences in the development of phoma stem canker epidemics between England and Poland relate to differences in weather patterns between the two countries. The duration of ascospore release was longer in England, where winter weather is mild and wet, than in Poland, where winters are cold and often with snow cover, but there was little difference between two sites in England (Rothamsted and Boxworth). Wetness provided by rainfall was essential for release of ascospores of both L. maculans in England and L. biglobosa in Poland. Temperature did not affect release of ascospores over the range 5–20 °C. Diurnal periodicity in release of ascospores of L. maculans in England and L. biglobosa in Poland was similar. The timing (date) of first release of ascospores of L. maculans or L. biglobosa in autumn was related to rainfall in August and September; with increasing rainfall the date was earlier. The incubation periods from first release of ascospores to first appearance of phoma leaf spots for both L. maculans in England and L. biglobosa in Poland, and from first leaf spots to first stem base canker in England, were described using a thermal time (degree-day) approximation. 相似文献
155.
The effect of breed of sire and age at feeding on muscle tenderness in the beef chuck 总被引:1,自引:0,他引:1
K L Christensen D D Johnson R L West T T Marshall D D Hargrove 《Journal of animal science》1991,69(9):3673-3678
Steers (n = 59) produced from the mating of Braford, Simbrah, Senepol, and Simmental bulls to Brahman- and Romana Red-sired cows and Brahman bulls to Angus cows were used in this study. Effects of sire breed and age at feeding on muscle tenderness in the major muscles of the chuck when steers were fed to 1.0 cm 12th rib fat were determined. There were no muscle tenderness effects due to sire breed group, with the exception of the serratus ventralis muscle, which was more tender in Brahman- and Braford-sired steers than in Simmental-sired steers. Additionally, the supraspinatus muscle from the yearlings was lower in shear value than that from the calves. The Brahman-sired steers had serratus ventralis muscles with higher percentages (P less than .05) of intramuscular fat than those of Braford-, Simbrah-, and Simmental-sired steers. Fat deposited within the muscle or between muscles in the chuck was not related to muscle tenderness as measured by Warner-Bratzler shear values. Also, percentages of intramuscular fat of the triceps brachii, serratus ventralis, or supraspinatus muscles were not influenced (P greater than .05) by age at feeding. 相似文献
156.
157.
158.
159.
Ellis J West K Cortese V Konoby C Weigel D 《Journal of the American Veterinary Medical Association》2001,219(3):351-356
OBJECTIVE: To determine the effect of maternally derived antibodies on induction of protective immune responses against bovine viral diarrhea virus (BVDV) type II in young calves vaccinated with a modified-live bovine viral diarrhea virus (BVDV) type I vaccine. DESIGN: Blinded controlled challenge study. ANIMALS: 24 neonatal Holstein and Holstein-cross calves that were deprived of maternal colostrum and fed pooled colostrum that contained a high concentration of (n = 6) or no (18) antibodies to BVDV. PROCEDURE: At 10 to 14 days of age, 6 seropositive and 6 seronegative calves were given a combination vaccine containing modified-live BVDV type I. All calves were kept in isolation for 4.5 months. Six calves of the remaining 12 untreated calves were vaccinated with the same combination vaccine at approximately 4 months of age. Three weeks later, all calves were challenged intranasally with a virulent BVDV type II. RESULTS: Seronegative unvaccinated calves and seropositive calves that were vaccinated at 2 weeks of age developed severe disease, and 4 calves in each of these groups required euthanasia. Seronegative calves that were vaccinated at 2 weeks or 4 months of age developed only mild or no clinical signs of disease. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that a single dose of a modified-live BVDV type-I vaccine given at 10 to 14 days of age can protect susceptible young calves from virulent BVDV type II infection for at least 4 months, but high concentrations of BVDV-specific maternally derived antibodies can block the induction of the response. 相似文献
160.
AIM: To evaluate the efficacy of ivermectin oral, moxidectin oral and moxidectin injectable formulations against an ivermectin-resistant strain of Trichostrongylus colubriformis in sheep. METHODS: Twenty-four mixed breed lambs were infected with 15,000 infective third-stage larvae of an ivermectin-resistant strain of T. colubriformis which had originally been isolated from a goat farm in Northland in 1997. Twenty-six days post infection, the lambs were divided into 3 treatment groups and a control group (n=6 lambs/group). Treatment consisted of either ivermectin oral formulation (0.2 mg/kg), moxidectin oral formulation (0.2 mg/kg), or moxidectin injectable formulation (0.2 mg/kg). Faecal egg counts (FECs) were determined at 0, 3, 5, 7 and 10 days after treatment. All animals were necropsied 12 days after treatment and worm counts were performed. Larval development assays were conducted 24 days post infection. A further 3 lambs were infected with 15,000 infective third-stage larvae of a fully susceptible strain of T. colubriformis for comparative purposes in the larval development assay. The efficacy of the moxidectin injectable formulation was also confirmed in these 3 lambs. RESULTS: The FEC reduction test at day 10 after treatment revealed 62%, 100% and 0% reductions in arithmetic-mean FECs for ivermectin oral, moxidectin oral and moxidectin injectable groups, respectively. The ivermectin oral, moxidectin oral and moxidectin injectable formulations achieved 62%, 98% and 4% reductions in arithmetic-mean worm burdens, respectively. Larval development assays showed resistance ratios for ivermectin of 4:1, avermectin B2 of 2.7:1, ivermectin aglycone of 37:1, moxidectin of 1.4:1, thiabendazole of 14.6:1 and levamisole of 1.8:1. CONCLUSIONS: The moxidectin oral formulation provided a high degree of control against ivermectin-resistant T. colubriformis whereas the moxidectin injectable formulation had very low efficacy. Ivermectin aglycone was the analogue of choice for diagnosis of ivermectin resistance in T. colubriformis in the larval development assay. 相似文献