Changes in Bull Sperm Kinematics after Single Layer Centrifugation

The objective of this study was to evaluate bull sperm kinematics after centrifugation through a single layer of a colloid [Single Layer Centrifugation (SLC)]. Ejaculates from 20 bulls were extended and stored at 4–6°C for 24 h during transport to the laboratory for SLC through Androcoll‐B, followed by measurement of sperm kinematics in all samples. Total motility (86% and 88% for uncentrifuged and SLC samples, respectively) and progressive motility (84% for both the groups) were similar (p > 0.05). In contrast, straightness (STR) (0.65 vs 0.69), linearity (LIN) (0.32 vs 0.35) and beat cross frequency (BCF) (22.3 vs 23.6 Hz) were significantly higher in the SLC‐selected samples than in the uncentrifuged samples, whereas velocity of the average path (VAP) (95 vs 90 μm/s), curvilinear velocity (VCL) (192 vs 180 μm/s), amplitude of lateral head deviation (ALH) (7 μm vs 6.5 μm) and hypermotility (49% vs 38%) were significantly decreased. The kinematics of the samples with the poorest motility was improved most by SLC. In conclusion, even though SLC had no direct effect on total and progressive motility, it appeared to have a positive influence on several other kinematic parameters that may be important for fertilization after artificial insemination.     

Detection and Characterization of the Cumulene Carbenes H2C5 and H2C6

Two cumulene carbenes, H2C5 and H2C6, were detected in a supersonic molecular beam by Fourier transform microwave spectroscopy. Their rotational and leading centrifugal distortion constants were determined with high accuracy, such that the entire radio spectrum can now be calculated. Like the known carbenes H2C3 and H2C4, both molecules have singlet electronic ground states and linear carbon-chain backbones. They can be produced in sufficiently high concentrations in the laboratory that their electronic spectra, expected to lie in the visible, should be readily detectable by laser spectroscopy. The microwave spectra of other, more exotic isomers may be detectable as well.     

Laser Rapid Prototyping of Photonic Band-Gap Microstructures

Three-dimensional periodic microstructures of aluminum oxide, which are important for creating photonic band-gap structures (PBGs), were fabricated by laser rapid prototyping by means of laser-induced direct-write deposition from the gas phase. The structures consisted of layers of parallel rods forming a face-centered tetragonal lattice with lattice constants of 66 and 133 micrometers. These structures showed transmission minima centered around 4 terahertz (75 micrometers) and 2 terahertz (150 micrometers), respectively. PBGs will allow precise control of the optical properties of materials, including lasers without threshold.     

Long-term tillage system effects under moist cool conditions in Switzerland

How do different soil tillage systems influence soil quality over the years? Under moist cool conditions is it possible in the long term to reduce dramatically soil tillage intensity without experiencing reductions in yield or other problems? In 1987, the Swiss Federal Research Station for Agricultural Economics and Engineering in Tänikon initiated a long-term soil tillage trial to clarify these questions. The trial compared mouldboard plough, chisel, paraplow, shallow tillage and no-tillage systems on a well-drained Orthic Luvisol with 160 g kg⁻¹ clay, 310 g kg⁻¹ silt, and under a climate that has a mean annual precipitation of 1180 mm. The tillage treatment effects were evaluated by measuring several biological, chemical, and physical soil quality indicators. Reduced soil tillage increased earthworm populations, reduced Pseudocercosporella herpotrichoides infection in wheat (Triticum aestivum) and increased plant colonisation by arbuscular mycorrhizal fungi. Yields for no-tillage and other ploughless cultivation techniques were on par with those obtained by ploughing. An exception was direct-drilled maize (Zea mays), where no-tillage decreased yield by more than 10% over the course of 14 years. In the first 7 years of the trial, the level of soil organic carbon in all the tillage regimes was approximately 40% lower than natural grassland (initial situation 1987=75 Mg SOC ha⁻¹). The no-tillage method did not differ from the others in respect of bulk density, but it showed an increased preconsolidation stress and hence better trafficability. Under Switzerland’s moist cool climatic conditions, it is possible to reduce soil tillage intensity without substantial reductions in yield, and at the same time improve soil quality.     

Successful treatment of hypereosinophilic syndrome in a dog

An 11-year-old crossbred dog was presented with a history of episodic vomiting, diarrhoea, abdominal discomfort and coughing. These signs had been present for several years. Marked peripheral eosinophilia (10.3 x 10(9)/L) was found. No underlying cause of reactive eosinophilia was apparent. Bone marrow aspiration biopsy showed hyperplasia of the eosinophilic cell line, with some increase in immaturity, although all cells were morphologically normal. There were numerous eosinophils in bronchial wash fluid and eosinophilic infiltrates were evident in biopsies of pancreas, liver, small intestine and colon but not stomach. Hypereosinophilic syndrome was diagnosed. The dog responded well to twice daily treatment with hydroxyurea and prednisolone. Clinical signs are well-controlled 16 months later.     

Prevention strategies for field traffic-induced subsoil compaction: a review: Part 2. Equipment and field practices

The loads imposed by modern farm machinery have considerable potential to increase subsoil stress. Within the context of economically viable and environmentally sustainable systems, the practices associated with subsoil damage and methods for avoidance are identified. The greatest potential for damage is on fragile, wet or loosened subsoils combined with high wheel or track loads and contact pressures that create noticeable ruts in the topsoil. In-furrow ploughing increases this potential considerably by placing loads on the subsoil. Measures to avoid this potential involve a whole farm approach and an understanding of the many interactions between cropping systems and machinery. Alternatives to in-furrow ploughing that involve working from the surface and building a protective topsoil are discussed. Key measures to reduce the risk to subsoils involve a clear understanding of tyre load and inflation data and simple on-farm methods of achieving this are suggested. Although avoidance has the potential to reduce the risk, confinement of damage to specific strips in the field is seen as a realistic alternative. Controlled traffic operations, together with precision guidance, offer an economic means by which compaction on the cropped area can be avoided. The most effective route to improvement in soil care across the European Union (EU) is an appropriate management structure coupled with a best practice framework.     

Concurrent gastro-oesophageal intussusception,trichobezoar and hiatal hernia in a cat

CASE HISTORY: An adult male Birman cat was evaluated for recurrent, intermittent vomiting or regurgitation, occasionally associated with abdominal discomfort.

CLINICAL FINDINGS AND DIAGNOSIS: Radiographs, including an oesophogram, indicated an oesophageal obstruction. Prior to treatment, the cat's condition deteriorated and it was euthanised at the owner's request. Post-mortem examination revealed a gastro-oesophageal intussusception, a trichobezoar impacted into the intussusceptum, and a dilated oesophageal hiatus consistent with a chronic hiatal hernia.

CLINICAL RELEVANCE: Gastro-oesophageal intussusception is a rare condition in cats. Its aetiology in relation to a pre-existing hiatal hernia and a trichobezoar is discussed.