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排序方式: 共有684条查询结果,搜索用时 15 毫秒
41.
Prinn RG Huang J Weiss RF Cunnold DM Fraser PJ Simmonds PG McCulloch A Harth C Salameh P O'Doherty S Wang RH Porter L Miller BR 《Science (New York, N.Y.)》2001,292(5523):1882-1888
The hydroxyl radical (OH) is the dominant oxidizing chemical in the atmosphere. It destroys most air pollutants and many gases involved in ozone depletion and the greenhouse effect. Global measurements of 1,1,1-trichloroethane (CH3CCl3, methyl chloroform) provide an accurate method for determining the global and hemispheric behavior of OH. Measurements show that CH3CCl3 levels rose steadily from 1978 to reach a maximum in 1992 and then decreased rapidly to levels in 2000 that were lower than the levels when measurements began in 1978. Analysis of these observations shows that global OH levels were growing between 1978 and 1988, but the growth rate was decreasing at a rate of 0.23 +/- 0.18% year(-2), so that OH levels began declining after 1988. Overall, the global average OH trend between 1978 and 2000 was -0.64 +/- 0.60% year(-1). These variations imply important and unexpected gaps in current understanding of the capability of the atmosphere to cleanse itself. 相似文献
42.
R Weiss 《Science (New York, N.Y.)》1991,252(5010):1059-1060
43.
Khaitovich P Hellmann I Enard W Nowick K Leinweber M Franz H Weiss G Lachmann M Pääbo S 《Science (New York, N.Y.)》2005,309(5742):1850-1854
The determination of the chimpanzee genome sequence provides a means to study both structural and functional aspects of the evolution of the human genome. Here we compare humans and chimpanzees with respect to differences in expression levels and protein-coding sequences for genes active in brain, heart, liver, kidney, and testis. We find that the patterns of differences in gene expression and gene sequences are markedly similar. In particular, there is a gradation of selective constraints among the tissues so that the brain shows the least differences between the species whereas liver shows the most. Furthermore, expression levels as well as amino acid sequences of genes active in more tissues have diverged less between the species than have genes active in fewer tissues. In general, these patterns are consistent with a model of neutral evolution with negative selection. However, for X-chromosomal genes expressed in testis, patterns suggestive of positive selection on sequence changes as well as expression changes are seen. Furthermore, although genes expressed in the brain have changed less than have genes expressed in other tissues, in agreement with previous work we find that genes active in brain have accumulated more changes on the human than on the chimpanzee lineage. 相似文献
44.
Kim M Hohman JN Cao Y Houk KN Ma H Jen AK Weiss PS 《Science (New York, N.Y.)》2011,331(6022):1312-1315
The products of photoreactions of conjugated organic molecules may be allowed by selection rules but not observed in solution reactions because of unfavorable reaction geometries. We have used defect sites in self-assembled alkanethiolate monolayers on gold surfaces to direct geometrically unfavorable photochemical reactions between individual organic molecules. High conductivity and stochastic switching of anthracene-terminated phenylethynylthiolates within alkanethiolate monolayers, as well as in situ photochemical transformations, have been observed and distinguished with the scanning tunneling microscope (STM). Ultraviolet light absorbed during imaging increases the apparent heights of excited molecules in STM images, a direct manifestation of probing electronically excited states. 相似文献
45.
F-actin and microtubule suspensions as indeterminate fluids 总被引:12,自引:0,他引:12
The viscosity of F-actin and microtubule suspensions has been measured as a function of shear rate with a Weissenberg rheogoniometer. At shear rates of less than 1.0 per second the viscosity of suspensions of these two structural proteins is inversely proportional to shear rate. These results are consistent with previous in vivo measurements of the viscosity of cytoplasm. This power law implies that shear stress is independent of shear rate; that is, shear stress is a constant at all shear rates less than 1.0 per second. Thus the flow profile of these fluids is indeterminate, or nearly so. This flow property may explain several aspects of intracellular motility in living cells. Possible explanations for this flow property are based on a recent model for semidilute suspensions of rigid rods or a classical friction model for liquid crystals. 相似文献
46.
Coronavirus infection induces H-2 antigen expression on oligodendrocytes and astrocytes 总被引:8,自引:0,他引:8
Infection of the central nervous system by mouse hepatitis virus strain A59, a murine neurotropic coronavirus, induces class I major histocompatibility complex antigens on mouse oligodendrocytes and astrocytes, cells that do not normally express these antigens on their surfaces. This induction, which occurs through soluble factors elaborated by infected glial cells, potentially allows immunocytes to interact with the glial cells and may play a critical role in the pathogenesis of virus-induced, immune-mediated demyelination in the central nervous system. 相似文献
47.
A persistent untranslated sequence within bacteriophage T4 DNA topoisomerase gene 60 总被引:30,自引:0,他引:30
W M Huang S Z Ao S Casjens R Orlandi R Zeikus R Weiss D Winge M Fang 《Science (New York, N.Y.)》1988,239(4843):1005-1012
A 50-nucleotide untranslated region is shown to be present within the coding sequence of Escherichia coli bacteriophage T4 gene 60, which encodes one of the subunits for its type II DNA topoisomerase. This interruption is part of the transcribed messenger RNA and appears not to be removed before translation. Thus, the usual colinearity between messenger RNA and the encoded protein sequence apparently does not exist in this case. The interruption is bracketed by a direct repeat of five base pairs. A mechanism is proposed in which folding of the untranslated region brings together codons separated by the interruption so that the elongating ribosome may skip the 50 nucleotides during translation. The alternative possibility, that the protein is efficiently translated from a very minor and undetectable form of processed messenger RNA, seems unlikely, but has not been completely ruled out. 相似文献
48.
Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line 总被引:34,自引:0,他引:34
P Clapham K Nagy R Cheingsong-Popov M Exley R A Weiss 《Science (New York, N.Y.)》1983,222(4628):1125-1127
Human T-cell leukemia virus (HTLV), American PL isolate, was transmitted by cocultivation and by cell-free filtrates to a nonlymphoid human osteogenic sarcoma (HOS) cell line, designated HOS/PL, but not to nine other lines bearing receptors for HTLV. HOS and HOS/PL cells are not dependent on interleukin-2 and do not express interleukin-2 receptors that are recognized by anti-Tac monoclonal antibody. HTLV released by the Japanese MT2 cell line was also transmitted to HOS cells. The infected HOS cells release substantial titers of progeny HTLV which is antigenically indistinguishable from parental virus and is able to transform T cells. 相似文献
49.
Fluorescence spectroscopy of single biomolecules 总被引:1,自引:0,他引:1
Weiss S 《Science (New York, N.Y.)》1999,283(5408):1676-1683
Recent advances in single-molecule detection and single-molecule spectroscopy at room temperature by laser-induced fluorescence offer new tools for the study of individual macromolecules under physiological conditions. These tools relay conformational states, conformational dynamics, and activity of single biological molecules to physical observables, unmasked by ensemble averaging. Distributions and time trajectories of these observables can therefore be measured during a reaction without the impossible need to synchronize all the molecules in the ensemble. The progress in applying these tools to biological studies with the use of fluorophores that are site-specifically attached to macromolecules is reviewed. 相似文献
50.
Since the formation of COMSAT 20 years ago, the number of international telephone circuits made through satellites has grown 400 times and the cost of a telephone call has decreased by 12,000 percent. Worldwide communications linking 109 nations are achieved with 14 satellites in earth-synchronous orbit. Advances in microwave technology have expanded the transmission bandwidth per satellite from 50 to more than 3000 megahertz. Improvements in solar cell, battery, stationkeeping, and microwave amplification technology have increased satellite lifetime from 1.5 years to 10. The sixth generation of INTELSAT satellites, now being manufactured, will be introduced into the system in 1986, and each satellite will carry more than 40,000 telephone channels plus two television programs. The next generation of satellites, now under design for expanded capacity, will be needed by the middle 1990's to meet the traffic demand. These satellites are likely to add new frequencies (20 and 30 gigahertz), onboard signal processing and switching, and more bandwidth-efficient modulation, to achieve larger communication capacity at each orbital location. 相似文献