鸭脂肪型脂肪酸结合蛋白基因（A-FABP）内含子1序列多态性分析

根据鸭A-FABPmRNA序列和鸡A-FABP基因组序列设计1对引物扩增出鸭脂肪型脂肪酸结合蛋白基因(A-FABP)内含子1序列(GenBank登录号:FJ536257),并根据扩增产物设计4对引物,利用PCR-SSCP对鸭A-FABP基因部分序列进行SNP检测,且对不同鸭群体进行群体遗传学分析.结果表明:克隆序列包含鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与鸡A-FABP基因内含子1同源性为75.1%;经SSCP检测,发现引物P1扩增片段有3处碱基突变,引物P3扩增片段有6处碱基突变,这些突变分别产生了3种和8种基因型.在P1位点樱桃谷鸭和苏牧麻鸭偏离了Hardy-Weinberg平衡(P0.05),在P3位点3个群体均符合Hardy-Weinberg平衡(P＞0.05).同时,这些基因型在不同鸭群体中分布存在显著或极显著差异(P<0.05或P<0.01).群体遗传学分析结果显示A-FABP基因在不同鸭群体中具有丰富的单核苷酸多态性,可以进一步作为候选基因来分析其与脂肪性状相关性.     

Effects of Ice Structuring Proteins on Freeze‐Thaw Stability of Corn and Wheat Starch Gels

The effect of ice structuring proteins (ISP, 0.5%, starch weight basis) on the freeze‐thaw stability of corn and wheat starch gels (CS and WS, respectively) was studied. Syneresis measurement, hardness, thermal properties using differential scanning calorimetry (DSC), X‐ray diffractions, and scanning electron microscopy (SEM) were determined with starch gels (SG) subjected up to nine freeze‐thaw cycles (FTC). The gelatinized SG were stored at –20°C for 22 hr and thawed at 30°C for 2 hr. As the number of FTC increased, syneresis was increased; however, ISP significantly (P < 0.05) reduced the syneresis for all FTC except at FTC 1. Hardness was increased up to FTC 4, then decreased for both SG. ISP significantly lowered the hardness of both SG. The ice melting enthalpy (ΔH_{ice sample}) of SG increased as FTC increased but SG with ISP had significantly lower enthalpy. The retrogradation ratio (RR) from DSC was significantly increased as the number of FTC increased. ISP reduced RR but most of RR was not significantly different for the corresponding FTC. The X‐ray diffraction patterns showed less recrystallization of WS with addition of ISP. The microstructures from SEM showed that the addition of ISP reduced the size of ice cell cavities.     

乙醇脱氢酶（ADH）家族生物信息学分析

本研究用生物信息学的方法分析了玉米等7个物种ADH的保守功能域、蛋白二级结构和进化关系。分析证实,植物ADH通常含有3个保守功能域,它们具有GroES结构的ADHN结构域,Rossmann折叠NAD（P）（＋）结合蛋白和锌结合位点,这3个保守结构在物种中具有相当的保守性。二级结构的分析表明,在我们研究的物种中,每个物种的两个ADH同工酶折叠情况大体相同,物种间蛋白二级结构也趋向一致。通过构建系统进化树,分析了供试物种中ADH以及ADH两个同工酶间的进化关系。初步显示,在进化上,单、双子叶植物源白不同的ADH祖先。双子叶植物ADH在物种间具有差异;而在单子叶植物不同物种其ADH同工酶出现分化。     

Integrated control of citrus green and blue mold and sour rot by Bacillus amyloliquefaciens in combination with tea saponin

A strain of Bacillus amyloliquefaciens HF-01, isolated from citrus fruit surfaces, was screened for in vitro antagonism toward Penicillium digitatum and identified, based on Biolog identification and phylogenetic analysis of 16S rDNA sequences. The isolate was further evaluated alone, or in combination with tea saponin (TS) on artificially inoculated ‘Wuzishatangju’ mandarin fruit. The results showed that the isolate performed significantly better than the water control in reducing the incidence of green and blue mold and sour rot, but was not as effective as the fungicide treatment. Biocontrol activity of B. amyloliquefaciens HF-01 was significantly improved by addition of TS, which might influence the formation of a bacterial biofilm and stimulate the antagonist population in wounds. The treatment comprising HF-01 combined with 50 μg mL^?1 TS was as effective as the fungicide treatment, which gave more than 90% control of green and blue mold and sour rot. B. amyloliquefaciens HF-01 alone or in combination with a low dosage of TS significantly reduced postharvest decay without impairing any of the other fruit quality parameters. The combination of B. amyloliquefaciens HF-01 and TS could be an alternative to synthetic fungicides for the control of citrus postharvest diseases.     

一类具有广义次临界条件的Kirchhoff方程解的存在性与多重性

研究了一类Kirchhoff型方程-(a+b∫RN|▽u|2dx)Δu+V（x）u=f（x,u）x∈RN利用变分方法获得方程解的存在性和多解性定理,改进和统一了相关结论.     

吹扫捕集/气相色谱-质谱法测定水中氯苯类化合物

建立了吹扫捕集/气相色谱-质谱(GC-MS)法测定水中氯苯类化合物的方法.通过对吹扫温度和吹扫时间、解吸温度和解吸时间进行优化,分析吹扫捕集条件对吹扫捕集效率的影响,并确定最佳吹扫捕集条件.结果表明,在最佳条件下,5种氯苯类化合物的相关系数为0.999 1~0.999 9,平均加标回收率为94.0%~107.2%,相对标准偏差为4.4%~6.1%,检出限为0.05~0.13μg/L.该方法与国家标准方法相比,操作简便、检出限低、准确度和精密度高,适用于地表水和生活饮用水中氯苯类化合物的分析测定.     

The Study of Engineering Drawings Scan Input and Vectiorization Svstem

We atternpt to describe an Engineering drawings scan and vectorization inputsystem,which can send to drawings into a computer and transfer the resulting data to commerciallyavailable CAD/CAM systems by image processing and pattern recognization; Some results processedby the system are given in the paper.     

An Estimating Method of Ultimate Gain and Ultimate Period

At tuning PID controller,ultimate gain Ku and ultimate period Tu of the proceseare usually the desired parameters.To lessen disturbance of tuning action and av oid to drive thesvstem to the verge of instability,a new estimating methed of Ku and Tu is developed,which based onthe charactristic areas of the u nit step response of the procase,By this method,it is easy forauto-tuning PID controller with Ziegler-Nichols like tuning formula. Results of digital simulationshow that this method is effective.     

中国尖尾叶蝉属一新种记述（半翅目：叶蝉科：广头叶蝉亚科）

该文记述了采自中国云南省的尖尾叶蝉属1新种,箭茎尖尾叶蝉Pedionis(Pedionis)sagittatasp.nov.,并提供了该种的整体图及雄性外生殖器的鉴别特征图.新种与李氏尖尾叶蝉P.(P.)lii Zhang et Viraktamath近似,区别特征为雄性尾节侧瓣腹缘具短刺毛;阳茎干突起基部较大,占据大半阳茎干;阳茎干强烈弯曲,相对较粗壮.     

连续UV-B胁迫对绿色型豌豆蚜生物学特性的影响

为了明确连续UV-B胁迫对绿色型豌豆蚜生长发育及种群参数的影响,将12 h内所产绿色型豌豆蚜若蚜经40 W UV-B辐射连续处理8代,每代6次,每天1次,每次分别处理20 min、30 min、40 min、50 min和60 min,每次处理后单头饲养,最终采用实验种群生命表方法进行评价,获得多代种群内禀增长率(rm)、净增殖率(R0)、平均世代周期(T)和周限增长率(λ)等生命参数。结果显示,随着辐射时间延长,世代数增加,各处理下若蚜历期最终延长,最长为8.11 d,成蚜寿命先延长后缩短,F8代为对照的6.71%~21.27%;净增殖率先升高后降低,F8代为对照的0.20%~22.32%;内禀增长率在F8代时显著降低,最高为对照的11.05%;周限增长率先升高后缩短,F8代为对照的0.90%~22.14%;F8代平均世代周期显著缩短,相对于对照减短8.54%~21.11%。连续UV-B胁迫试验中,处理时间越长,随着世代数增多,UV-B对绿色型豌豆蚜生长发育和繁殖抑制作用越强,种群数量增长越慢。