Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

20世纪80年代以来世界除草剂新品种开发进展及特点(一)

综述了近20年来世界除草剂新品种的开发进展，并介绍了除草剂工业发展的一些新特点。     

BsC3-41杀蚊幼制剂对蚊幼虫毒杀效果研究

通过以BsC3- 41杀蚊幼制剂对 3种蚊幼虫进行生物活性测定和野外灭蚊试验 ,结果表明 :该制剂对致乏库蚊CulexfatigansWiedemann的毒杀效果最好、对中华按蚊AnophelessinensisWiedemann次之、对白纹伊蚊AedesalbopictusSkuse的效果较差 ,2 4小时LC50值分别为 0 2 0 2 5 μg/ml、 2 5 363μg/ml和 5 9 730 2 μg/ml。野外水体灭蚊使用 3ml/m2 的浓度防治淡色库蚊效果可达 98 88%～ 1 0 0 0 0 % ,使用 1 0ml/m2 的浓度防治中华按蚊效果可达96 81 %～ 1 0 0 0 0 % ,使用 2 0 0ml/m2 的浓度防治白纹伊蚊效果达到 90 64%以上。     

蛋鸡血浆VLDL浓度二世代双向选择对体脂沉积的影响

以雅发褐壳蛋鸡为素材,在零世代和一世代双向选择的基础上,应用血浆极低密度脂蛋白(VLDL)浓度对体腹脂性状继续进行双向选择,比较了高低脂系间体组成的差异,并研究了中后期血浆VLDL浓度与上述性状的相关程度,进一步探讨了血浆VLDL浓度的选择效应。结果表明:二世代母鸡中后期高脂系体重较高(P<0.05),血浆VLDL浓度与体重呈极显著的表型相关(P<0.01)和中等程度的正遗传相关。62周龄高脂系的腹脂率显著高于低脂系(P<0.05),说明运用血浆VLDL浓度经过二个世代的双向选择,使产蛋鸡中后期体重降低,且对腹脂沉积产生了一定的影响。     

饲喂柠条对肉兔生产性能及胴体性状影响的研究

选用45～48日龄平均体重1050g的伊普吕(父系)幼兔64只,分成4组,进行柠条饲喂肉兔试验.对照组饲粮中谷草比例为33.4%,试验Ⅰ、Ⅱ、Ⅲ组分别用10%、20%和33.4%柠条替代等比例谷草.48天试验结果为:对照组、试验Ⅰ、Ⅱ、Ⅲ组日均采食量、日增重、料肉比分别为89.84g、92.95g、90.77g、79.95g,22.60g、23.30g、19.24g、17.90g,3.98:1、3.99:1、4.72:1、4.47:1.试验Ⅰ、Ⅱ组与对照组日采食量、日增重、料肉比差异不显著(P＞0.05);屠宰试验结果为:试验Ⅰ、Ⅱ与对照组在胴体重、屠宰率、净肉率、肉骨比、心脏、肺脏、肝脏、肾脏等指标方面无显著差异(P＞0.05);而试验Ⅲ组日采食量、日增重均显著较对照组差(P＜0.05).试验结果表明:生长肉兔饲粮中添加10%～20%柠条粉是可行的,且饲粮成本较低.     

香精油抗蜂螨作用的研究进展

香精油及其组分具有显著的抗螨效果。萜类化合物(主要是单萜)是香精油的主要成分。占总量的90％左右。研究者通过筛选试验测定了多种香精油及其一些组分的抗螨能力。大部分表现出良好的抗螨效果。但只有少部分在蜂群试验中表现出强抗螨能力。因此，有必要对此作进一步的研究。以达到最优化目标。同时联合其它防治手段，建立一套全面的害虫防治策略，实现蜂螨的最终控制。     

Preliminary results of an anticircumsporozoite DNA vaccine trial for protection against avian malaria in captive African black-footed penguins (Spheniscus demersus).

Captive juvenile African black-footed penguins (Spheniscus demersus) housed in an outdoor enclosure at the Baltimore Zoo have an average 50% mortality from avian malarial (Plasmodium sp.) infection each year without intense monitoring for disease and chemotherapeutic intervention. During the 1996 malaria transmission season, the safety and efficacy of an anti-circumsporozoite (CSP) DNA vaccine encoding the Plasmodium gallinaceum CSP protein against P. relictum were studied. The goal was to reduce clinical disease and death without initiating sterile immunity after release into an area with stable, endemic avian malaria. The birds were monitored for adverse clinical signs associated with vaccination, the stimulation of an anti-CSP antibody response, and protection afforded by the vaccine. The presence of P. relictum in trapped culicine mosquitoes within the penguin enclosure was monitored to assess parasite pressure. Among the vaccinated penguins, the parasitemia rate dropped from approximately 50% to approximately 17% despite intense parasite pressure, as determined by mosquito infection rate. During the year of the vaccine trial, no mortalities due to malaria occurred and no undesirable vaccination side effects occurred. This is the first trial of an antimalarial vaccine in a captive penguin colony.     

pH及阳离子浓度对柞蚕蛹蛋白溶解性的影响

对不同pH及阳离子浓度下柞蚕蛹蛋白的溶解性进行了研究。结果表明,柞蚕蛹蛋白的等电点pI为4.2~4.5。测定不同的阳离子对其溶解度的影响顺序为Zn2+>Ca2+>Mg2+>Na+,随着阳离子浓度的增加,蛹蛋白的溶解性增加,当离子浓度超过1.0 mol/L时,其溶解性则降低。     

动物生物钟基因研究进展

昼夜节律生物钟是一种以近似24小时为周期的自主维持的振荡器,由输人通路、中央振荡器和输出通路三部分组成的。生物钟机制的研究已深入到分子水平。生物钟相关基因相继被分离鉴定,它们及其编码的蛋白质产物构成的自主调节的转录和翻译反馈环是生物钟运转的分子机制。本文介绍了果蝇和小鼠主要生物钟基因的发现以及其突变体对生物昼夜节律的影响,展望了揭示生物钟调节机制在遗传学上的重要意义。     

永失我爱--祭爱犬丁丁

2005年2月底3月初的那段日子是我最背霉运，最不顺利的。3月7日中午，我忽然看到一张贴子，说有一窝（5头）小喜乐蒂犬待售，从来没有养过犬的我忽然间对此产生了浓厚的兴趣。这时丁丁就跃入我的眼帘：憨态可拘的他有着一双清澈的眼睛，努力睁得大大的试图将一切事物尽收眼底；一身柔软的小绒毛看上去活象一个小玩具，更让我对他注意的应该算是他又宽又通的鼻线，再加上小脑门上有一个好似菱形的小胎记。好，就是他了，我对自己说。