苏云金芽孢杆菌cry基因在大肠杆菌中表达产物和生物活性

研究了几种Bt cry基因于大肠杆菌(Escherichia coli)中表达产物在pH 10.0的50mmol/L碳酸钠和20mmol/L乙醇胺溶解液中的溶解性 ,发现同样的Cry蛋白在碳酸钠中的溶解度大于乙醇胺。通过胰蛋白酶消化 ,明确Cry1Ca7、Cry1Ia8酶解产物为 38kD多肽 ;Cry1Ie1、Cry1Cb2、Cry2Ab4酶解产物为 41kD多肽 ;Cry1Ac酶解产物为60kD多肽。采用FPLC层析方法对 6种原毒素及其酶解后得到的毒素多肽进行了分离纯化 ,比较了原毒素和毒素的杀虫活性的差异。其结果表明 ,Cry1Ac的原毒素和毒素对棉铃虫初孵幼虫的校正死亡率均为 100% ,Cry2Ab4的原毒素的毒力高于其酶解毒素。     

Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts

AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK.     

Effects of Taoren Honghua Jian on the hyperlipidemia without symptom

AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level.     

达县分葱田甜菜夜蛾发生规律初步研究

采用系统调查方法研究了达县地区分葱田甜菜夜蛾的生活史、发生与环境的关系。甜菜夜蛾在达县分葱田1a发生6代,以三至五代为害最重,世代重叠。其数量消长与虫源、夏秋季气候、天敌、栽培管理等因素密切相关。通过调查分析提出本县初始虫源非本地虫源。     

利用RT-PCR与PVP-ELISA检测水稻瘤矮病单头叶蝉

 目前国内外对水稻瘤矮病毒(Rice gall dwarf virus,RGDV)的研究主要集中在其核衣壳蛋白和外衣壳蛋白的性质、结构及其与介体的亲和性、与介体传毒能力的关系等方面,本室在已有室内检测研究的基础上,利用RT-PCR和PVP-ELISA法对田间带毒叶蝉进行检测,并对2种方法的检测结果进行了比较。     

大白菜细胞核雄性不育基因向自交系97A407的转育

根据复等位基因遗传假说 ,利用大白菜核不育系 9810 9和已知基因型的甲型两用系可育株 99135 2作为不育源 ,以大白菜自交系 97A40 7为目标亲本 ,经过 5个世代的转育 ,获得了具有 97A40 7遗传基因型的新甲型两用系、临时保持系和雄性不育系。     

Dose response of weeds to methyl iodide and methyl bromide

Labonuory bioassay and field experiments were conducted to characterize the dose response of weeds to methyl iodide and methyl bromide as soil fumigants. The patterns in potency of both fumigants and in sensitivity of diffcretit weed species to the fumigants were distinguished with the use of logistic dose-response models. Similar to its response to methyl hromide fumigation. Amaranthus retrofleus L. was the most sensitive to methyl iodide fumigation. Cyperus rotundtis L. was the least sensitive to methyl iodide fumigation, whereas Portuloca oleracea L. was the least sensitive to methyl hromide. Lolium multiflorum Lam. Abutilon theophrasti Medik. Chenopodium album L. P. ateracea . Brassica kaber (D.C.) L.C. Wheeler and Cyperus escuden-tus L. were similar in sensitivity to methyl iodide. Methyl iodide was as potent as methyl bromide for A. retroflexus but more potent than methyl bromide for L. multiflorum , A. theophrasti. C. album. P. oleracea. B. kaber, C. esculentus and C. rotundus. The dose response for weeds in the field was similar to that obtained in laboratory bioassays. Under fieid conditions. 280 kg ha^-1 methyl iodide killed all species tested except Solanum nigrum L Methyl iodide appears to be a suitable replacement for meihyl bromide because it can be used in situations simitar to methyl bromide fumigation, has superior efficacy against a broad spectrum of pests and has a low potential for degrading the earth's ozone lavers.     

棉铃虫对拟除虫菊酯类杀虫剂抗性遗传力的分析

采用域性状分析法,估算了棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力,并对3种菊酯的抗性风险进行了评估。结果表明,棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力分别为0.2027、0.1554和0.1084。假设田间种群的抗性现实遗传力为估算值的一半,杀死率为80％,预计抗性增长到10倍时,氰戊菊酯可使用14次,三氟氯氰菊酯可使用19次,溴氟菊酯可使用31次。三氟氯氰菊酯和溴氟菊酯的抗性风险明显低于氰戊菊酯;棉铃虫对含氟菊酯的抗性发展速度明显低于不含氟的菊酯。试验结果为实际应用提供了理论依据。     

蜗牛液对不同果蔬病原菌的抑制及其细胞的溶解

蜗牛(Bradybaena kiangsinensis Martens)是我国的特有种群,它的腺体中含有多种生物活性物质。由于蜗牛在农业生产中具有一定的危害性,而忽视了其在生防中的作用。为探索蜗牛用于生防的可能性,作者对蜗牛提取液的抑菌和溶菌效果进行了研究。 1 材料与方法蜗牛液采用活体提取法,以无菌水作提取介质,用量为蜗牛量的30％,常温下浸提1h,然后过滤备用,以无菌水作提取液对照。抑菌试验采用含毒介质法,即取9ml PDA培     

奶牛"两病"的监测与思考

奶牛“两病”是指奶牛的结核病和布鲁氏菌病，是世界动物卫生组织(OIE)规定强制报告的两种疫病，二病都属人畜共患病。人的牛型结核感染主要是通过饮用生奶引起，尤其是儿童常因喝生牛奶被感染而患肠结核杆菌病；饮用生奶或制品也易感染布鲁氏菌病。奶牛“两病”严重威胁着人民的身体健