中辽1号杨在辽宁地区引种研究

对20世纪末新引进到辽宁并经初选的12个杨树无性系进行了引种试验研究,通过无性系多点生长对比试验、室内外物候观测、对杨干象抗性评价和材性测试等,筛选出生长快、适应强的杨树新品种中辽1号杨,其材积生长量分别超过当地对照种辽宁杨、沙兰杨、荷兰3930杨和I-214杨14.8%~68.1%。其生长进程中高生长最快期是在5~6年生,连年生长量最大数值达到3.6 m;胸径生长最快期是在3~5年生,连年生长量最大数值达到6.34 cm。而且干型好、材质优良,对杨干象有相对较强的抗性,可作为新品种在其适生区大面积推广。     

黔育1号菊苣与紫花苜蓿、鸭茅间作栽培效果研究

依据贵州省松桃县的气候特点和牧草种植需要,用黔育1号菊苣与北林202紫花苜蓿、黔草4号鸭茅分别进行间作试验,从产量、营养品质、生产效益等方面对间作复合群体与3种牧草单播进行比较分析,探讨黔育1号菊苣与不同牧草间作种植模式的增产效益。结果表明,在贵州松桃地区,黔育1号菊苣+北林202紫花苜蓿间作,年均鲜草产量可达87.27 t/hm²,比紫花苜蓿单播产量增加36.17%,生产效益提高20.77%,粗蛋白含量达26.83%。该研究结果可为松桃县种草养畜产业的发展提供技术支撑。     

便携式XRF仪现场快速测定土壤中Pb的可行性研究

便携式XRF分析仪具有轻便、快速和可测对象较多与测量元素较广等特点而广泛应用于矿石、土壤等方面快速检测,但土壤的现场快速测定结果的准确性有待进一步研究与探讨。以安宁河谷平原土壤中Pb为研究对象,通过对便携式XRF仪现场快速检测结果与实验室分析结果的对比和影响因素的分析,建立基于安宁河谷平原表层土壤快速测定结果的线性函数为y=1.170 9x-17.326 0(R~2=0.992 9,x为现场检测结果),探讨其影响因素,为该区域表层土壤中Pb快速检测结果提供依据。     

不同培养基成分对薄荷试管苗生根的影响

[目的]筛选薄荷试管苗生根的最佳培养基组合。[方法]以薄荷试管苗茎段为试验材料,探讨不同培养基成分、凝固剂种类及添加量对其生根的影响。[结果]在薄荷试管苗培养过程中,使用琼脂为凝固剂,且添加量为7 g/L的1/2MS培养基时,其生根时间最短,生根数和生根长度较高。[结论]为薄荷试管苗的大规模、优质、高效生产提供理论依据。     

基于PLC的碟式太阳自动跟踪控制系统研究

由于太阳辐射方向的时刻改变和太阳辐射能的不稳定性,导致碟式太阳能发电系统利用率始终较低。为了解决这个问题,本文提出了一种基于PLC控制的全天候自动跟踪方式,利用光照强度与光照强度阈值的差值作为跟踪方式切换的判据,不仅消除了程序跟踪造成的累积误差,而且避免了光电传感器在阴天或多云时无法工作的问题。为保证系统在任何天气情况下均可稳定运行,信息反馈元件增加了风速传感器、温度传感器及倾角传感器。另外,在设计系统硬软件时,采取了一系列措施来进一步降低系统运行成本。     

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.     

Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.     

中国不同品种谷子抗性淀粉分布规律

抗性淀粉是谷子中的重要功能成分。对来自中国4个生态区10个省份216个谷子品种抗性淀粉含量进行分析。结果表明,中国谷子地方品种抗性淀粉平均值为2.43%,含量变幅为0.00%~6.74%,变异系数为50.26%,呈偏正态分布;不同生态区谷子抗性淀粉含量从高到低依次为内蒙古高原、华北平原、东北平原、黄土高原,且内蒙古高原和黄土高原之间差异显著(P0.05);不同省份抗性淀粉含量存在一定差异,其中黑龙江谷子抗性淀粉含量显著高于内蒙古和甘肃(P0.05),其他省份间差异不大;不同省份谷子(黑龙江除外)和不同生态区品种谷子抗性淀粉含量以2.00%~4.00%为主。