New method for objectively assessing lameness in broiler chickens

A new method for assessing the severity of lameness in broiler chickens is described. The length of time that birds remained standing in shallow water was measured and the results were compared with the results of conventional gait scoring. There was a highly significant (P < 0.001) relationship between the birds' gait scores and their latency to lie down. Over 750 broilers aged between 32 and 45 days of age were tested in commercial houses and whereas nearly all the sound birds were able to stand for at least 15 minutes, the majority of lame birds lay down within five minutes.     

Internal roosting location is associated with differential use of the outdoor range by free-range laying hens

1. In commercial free-range systems for laying hens, popholes to the outdoor range are often installed on one side of the house only. In multi-tier systems, it is possible that some individuals fail to access the range due to internal barriers to movement.

2. Five commercial multi-tier flocks from different units were studied. For each flock, two different colour markers were used to distinguish 200 birds roosting near the popholes (NP-Roost) and 200 birds roosting far from the popholes (FP-Roost) at night. The following day, counts of marked birds on the range and inside the house were performed.

3. Significantly more NP-Roost birds were observed in all areas of the outdoor range than FP-Roost birds the next day. Distance of FP area from the popholes was very strongly positively correlated with effect size in the adjacent range area.

4. Additionally, in the indoor area far from the popholes (FP) more FP-Roost birds were observed the next day than NP-Roost birds. In the indoor area near to the popholes (NP) more NP-Roost birds were observed the next day than FP-Roost birds.

5. These results suggest that roosting location is associated with differential range use when popholes are only available on one side of the shed as birds that roosted far from the popholes used the range less.     

Partial tracheal obstruction due to chondromas in ball pythons (Python regius).

Over a 9-mo period, three adult ball pythons (Python regius) (one male, two females) were evaluated for severe dyspnea. Partial obstructions of the tracheal lumen were identified radiographically and/or visualized with a 3.0-mm rigid laparoscope inserted into the tracheal lumen in all three snakes. Administration of systemic antibiotics and nebulization resulted in partial improvement of the dyspnea. In two snakes, the tracheal lesions were removed with a rigid laparoscope and a flexible biopsy instrument inserted into the tracheal lumen. The other snake died and was necropsied. Histologically, the lesions from two snakes were determined to be benign chondromas. The chondromas were composed of a variably disorganized chondroid matrix populated by quiescent, normal-appearing chondrocytes within lacunae, although the chondrocytes were increased in density compared with normal hyaline cartilage and contained rare mitotic figures. The tracheal masses in one snake grew by expansion, not invasion, and were focally continuous with a mineralized cartilage tracheal ring, suggesting a benign nature. This is the second report of tracheal chondroma in ball pythons. Tracheal chondromas are exceedingly rare in humans and domesticated animals, suggesting a possible predisposition of ball pythons for this neoplasm.     

Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle

OBJECTIVE: To compare a recently developed recombinant MSP-5 competitive inhibition ELISA with a card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in Australian cattle. MATERIALS AND METHODS: The ELISA was compared with the card agglutination test using 208 sera from cattle in Anaplasma-free herds, 86 sera from cattle experimentally infected with A marginale or A centrale and 757 sera from cattle in areas endemic for A marginale. RESULTS: The specificity of the ELISA, based on testing 208 sera from cattle in Anaplasma-free areas, was 99.5%, and the sensitivities for detection of antibodies to A marginale and A centrale in sera from the experimentally infected cattle were 98.0% and 100%, respectively. For the same sets of sera, the specificity of the card agglutination test was 98.6% and the sensitivities for detection of antibodies to A marginale and A centrale were 98.0% and 100%, respectively. For the 757 sera collected from cattle in areas endemic for A marginale, the agreement between the ELISA and the card agglutination test depended on the positive threshold selected for the ELISA. The maximum achievable agreement was 91.5% (kappa = 0.73; 95% confidence interval 0.66, 0.79). CONCLUSION: We conclude that the competitive inhibition ELISA is a useful alternative to the card agglutination test for detection of A marginale or A centrale infection in cattle. The assay should be particularly useful for epidemiological applications such as prevalence studies and control programs.     

Quantum statistical corrections to dynamic nuclear magnetic resonance

A quantum statistical treatment of the chemical exchange between molecular eigenstates or conformations revealed previously unsuspected dynamic terms in the spin Hamiltonian operator that describes fast exchange. These terms resulted from the effect of nuclear spin on rotational and vibrational relaxation. With the traditional theory, an interpretation of new carbon-13 nuclear magnetic resonance measurements of the chemical shift of methylcyclohexane in solution showed fast-exchange equilibrium constants that were inconsistent with the slow-exchange free-energy difference and were spread over a range of 30 percent for the various carbon-13 positions. Modeling of the new terms indicated that they have the correct magnitude and temperature dependence to reconcile these inconsistencies.     

A neoproterozoic snowball earth

Negative carbon isotope anomalies in carbonate rocks bracketing Neoproterozoic glacial deposits in Namibia, combined with estimates of thermal subsidence history, suggest that biological productivity in the surface ocean collapsed for millions of years. This collapse can be explained by a global glaciation (that is, a snowball Earth), which ended abruptly when subaerial volcanic outgassing raised atmospheric carbon dioxide to about 350 times the modern level. The rapid termination would have resulted in a warming of the snowball Earth to extreme greenhouse conditions. The transfer of atmospheric carbon dioxide to the ocean would result in the rapid precipitation of calcium carbonate in warm surface waters, producing the cap carbonate rocks observed globally.     

Fine-needle aspirate cytology suggesting hepatic lipidosis in four cats with infiltrative hepatic disease

Four cats are reported in which cytology smears obtained by ultrasound-guided fine needle aspiration of the liver were interpreted as indicative of hepatic lipidosis. However, histopathology of hepatic tissue samples obtained with Tru-Cut-like needles or wedge biopsy revealed that the cats had inflammatory or neoplastic hepatic disease causing their clinical signs. Fine needle aspiration and cytology may not detect infiltrative lesions, particularly those that are nodular, multifocal, or localised around the portal regions. Fine needle aspirate cytology is a useful diagnostic procedure with many advantages, but care must be taken to avoid diagnosing hepatic lipidosis as the cause of illness when an infiltrative lesion is responsible.     

A new look at an old practice: Benefits from soil water accumulation in long fallows under Mediterranean conditions

The practice of long fallowing, by omitting a year of cropping, is gaining renewed focus in the low rainfall zone of the northern agriculture region of Western Australia. The impetus behind this practice change has been a reduced use of pasture breaks in cereal crop rotations, and the belief that a fallow can improve soil water accumulation and thus buffer the negative effects of dry seasons on crop yields. We evaluated the benefits of long fallowing (full stubble retention, no weed growth allowed) in a continuous wheat sequence via simulation modelling with APSIM at two rainfall locations and five soil types. The simulated benefits to long fallowing were attributable to soil water accumulation only, as the effects on soil nitrogen, diseases or weeds were not evaluated.The long-term (100 years) mean wheat yield benefit to fallowing was 0.36-0.43 t/ha in clay, 0.20-0.23 t/ha in sand and loam, and 0-0.03 t/ha in shallow sand and shallow loams. Over the range of seasons simulated the response varied from −0.20 to 3.87 t/ha in the clay and −0.48 to 2.0 t/ha for the other soils. The accumulation of soil water and associated yield benefits occurred in 30-40% of years on better soils and only 10-20% on poorer soils. For the loam soil, the majority of the yield increases occurred when the growing-season (May-September) rainfall following the fallow was low (<210 mm) and the difference in plant available soil water at sowing between fallowed and continuously cropped soil was high (>30 mm), although yield increase did occur with other combinations of growing-season rainfall and soil water. Over several years of a crop sequence involving fallow and wheat, the benefits from long fallowing due to greater soil water accumulation did not offset yield lost from omitting years from crop production, although the coefficient of variation for inter-annual farm grain production was reduced, particularly on clay soils during the 1998-2007 decade of below-average rainfall. We conclude that under future drying climates in Western Australia, fallowing may have a role to play in buffering the effects of enhanced inter-annual variability in rainfall. Investigations are required on the management of fallows, and management of subsequent crops (i.e. sowing earlier and crop density) so as to maximise yield benefits to subsequent crops while maintaining groundcover to prevent soil erosion.